本文關(guān)鍵詞：ITGA3在卵巢上皮細(xì)胞癌中的表達(dá)及意義　出處：《江蘇大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 上皮性卵巢癌 ITGA3 惡性程度

【摘要】：目的:通過(guò)檢測(cè)整合素亞單位α3(integrin subunitα3,ITGA3)在卵巢不同細(xì)胞系及組織中的表達(dá)水平,探討ITGA3與卵巢上皮細(xì)胞癌間的關(guān)系,并分析ITGA3的表達(dá)與患者臨床參數(shù)的相關(guān)性,為接下來(lái)研究其在卵巢癌發(fā)生、發(fā)展中的分子機(jī)制及靶向治療提供實(shí)驗(yàn)依據(jù)。方法:(1)利用實(shí)時(shí)熒光定量PCR技術(shù)(q RT-PCR)檢測(cè)ITGA3在正常卵巢上皮細(xì)胞系、各種卵巢癌細(xì)胞系、卵巢良性組織與卵巢上皮細(xì)胞癌組織中的m RNA表達(dá)水平。(2)免疫組化方法檢測(cè)ITGA3在良性卵巢組織與上皮性卵巢癌組織中的蛋白表達(dá)情況。本研究用病理切片來(lái)自江蘇大學(xué)附屬醫(yī)院、江蘇大學(xué)附屬人民醫(yī)院及連云港市第二人民醫(yī)院2013年至2016年術(shù)后病理存檔的卵巢組織,共65例,其中上皮性卵巢癌組織38例,卵巢良性組織27例。收集患者臨床資料,分析ITGA3的蛋白表達(dá)與卵巢癌患者臨床參數(shù)的相關(guān)性。結(jié)果:(1)ITGA3在SKOV3和OVCAR-3兩種卵巢癌細(xì)胞系中的m RNA表達(dá)水平高于正常卵巢上皮細(xì)胞HOSEPIC(P0.001)。(2)ITGA3在上皮性卵巢癌組織中的m RNA表達(dá)水平明顯高于卵巢良性組織(P0.001)。(3)免疫組化結(jié)果顯示:卵巢良性組織和上皮性卵巢癌組織中均有ITGA3蛋白的表達(dá),但卵巢良性組中僅有4例呈陽(yáng)性,而上皮性卵巢癌組中有26例陽(yáng)性表達(dá),兩組間差異具有統(tǒng)計(jì)學(xué)意義(P=0.000)。在病理分期越高、分化越差的卵巢癌組織中ITGA3蛋白的高表達(dá)越明顯(P=0.016,P=0.003)。結(jié)論:ITGA3有可能作為判斷上皮性卵巢癌惡性發(fā)展程度的標(biāo)志,為制定臨床治療方案提供參考依據(jù),并有可能對(duì)患者的預(yù)后作出評(píng)估和成為卵巢癌治療的靶點(diǎn)。
[Abstract]:Objective: to detect the expression of integrin subunit 偽 3ITGA3 in different ovarian cell lines and tissues. To investigate the relationship between ITGA3 and ovarian epithelial cell carcinoma, and to analyze the correlation between the expression of ITGA3 and the clinical parameters of the patients. Methods: using real-time fluorescence quantitative PCR technique to detect ITGA3 in normal ovarian epithelial cell line. Various ovarian cancer cell lines. Expression level of m RNA in benign ovarian tissue and ovarian epithelial cell carcinoma. Immunohistochemical method was used to detect the expression of ITGA3 in benign ovarian tissues and epithelial ovarian cancer tissues. From 2013 to 2016, 65 cases of ovarian tissues were recorded in the affiliated people's Hospital of Jiangsu University and the second people's Hospital of Lianyungang City, including 38 cases of epithelial ovarian cancer. The clinical data of 27 patients with benign ovarian tissue were collected. To analyze the correlation between the expression of ITGA3 protein and the clinical parameters of ovarian cancer patients. The expression of ITGA3 in SKOV3 and OVCAR-3 ovarian cancer cell lines was higher than that in normal ovarian epithelial cells. The expression of m RNA in epithelial ovarian carcinoma was significantly higher than that in benign ovarian carcinoma (P0.001. Immunohistochemical results showed that ITGA3 protein was expressed in benign ovarian tissues and epithelial ovarian cancer tissues. However, only 4 cases were positive in benign ovarian group and 26 cases in epithelial ovarian carcinoma group. The difference between the two groups was statistically significant. The higher the pathological stage was, the higher the pathological stage was. The higher the expression of ITGA3 protein in poorly differentiated ovarian cancer tissues, the more obvious the expression of ITGA3 protein was 0.016. Conclusion: TGA _ 3 may be used as a marker to judge the malignant development of epithelial ovarian cancer and provide reference for clinical treatment. It is possible to evaluate the prognosis of patients and become a target for ovarian cancer treatment.
【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R737.31

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本文編號(hào)：1395898