本文關(guān)鍵詞：山海螺化學(xué)成分與質(zhì)量控制研究　出處：《北京中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：本論文包括兩部分實(shí)踐部分和實(shí)驗(yàn)部分。論文第一部分為中日友好醫(yī)院藥學(xué)部實(shí)踐部分。分別是門診中藥房、住院中藥房、中藥庫、中藥制劑室、藥檢室。在這5個(gè)部門實(shí)踐過程中,學(xué)習(xí)和了解了各部門的工作流程及注意事項(xiàng)。論文第二部分為是山海螺的化學(xué)成分與質(zhì)量控制研究。主要分為三個(gè)部分,一、山海螺藥材的研究進(jìn)展;二、山海螺的化學(xué)成分研究;三、山海螺的質(zhì)量控制研究。第一部分對山海螺的化學(xué)成分、藥理作用、臨床應(yīng)用等方面進(jìn)行論述。闡述了山海螺的化學(xué)成分主要為三萜皂苷類化合物,此外,還含有糖類、甾醇、生物堿類、黃酮類及揮發(fā)油等化合物。具有抗癌、抗突變、抗氧化、降血糖、降血脂、鎮(zhèn)痛等作用。第二部分為山海螺的化學(xué)成分研究,采用HP-20大孔樹脂、Sephadex LH-20柱色譜等色譜分離方法對山海螺50%乙醇洗脫部位進(jìn)行化合物分離純化,通過1 H-NMR和13 C-NMR等波譜技術(shù)和化學(xué)方法鑒定所分離純化得到的化合物結(jié)構(gòu)。通過反復(fù)上SephadexLH-20柱色譜共分離得到6個(gè)化合物,鑒定了 5個(gè)化合物,分別是5-羥甲基-2-呋喃甲醛(1)、CodonopilodiynosideA(2)、lobetyol(3)、(+)-isolariciresinol(4)、黨參炔苷(5)。其中化合物(1)、(2)、(3)、(4)均為首次從該植物中分得。第三部分為山海螺的質(zhì)量控制研究,運(yùn)用HPLC建立黨參炔苷含量測定的方法學(xué),同時(shí)共收集了 13批山海螺藥材進(jìn)行HPLC測定,測得色譜圖,運(yùn)用中國藥典委員會"中藥色譜指紋圖譜相似度評價(jià)軟件A"進(jìn)行評價(jià),生成對照圖譜。該方法操作簡便快捷,具有專屬性強(qiáng)、穩(wěn)定性、重復(fù)性良好等特點(diǎn),可用于山海螺藥材的質(zhì)量控制與評價(jià)。
[Abstract]:This paper includes two parts of practice and experiment. The first part of the thesis is the practical part of the pharmacy department of China-Japan Friendship Hospital. They are the pharmacy in the outpatient department, the pharmacy in the hospital, the Chinese medicine library, the Chinese medicine preparation room and the medicine inspection room. During the practice of these 5 departments, we learn and understand the work flow and attention of various departments. The second part of the thesis is to study the chemical constituents and quality control of Codonopsis lanceolata. Mainly divided into three parts. The research progress of medicinal materials, a study on the chemical constituents of Codonopsis lanceolata; two, the research on the quality control of Codonopsis lanceolata; three, Codonopsis lanceolata. The first part of Codonopsis lanceolata chemical composition, pharmacological effects and clinical application were discussed. Describes the three main chemical composition of Codonopsis lanceolata saponins compounds, in addition, also contains carbohydrates, sterols, alkaloids, flavonoids and volatile oil compounds. It has the effect of anticancer, anti - mutation, antioxidation, hypoglycemic, antilipidemia and analgesic effect. The second part is the study on the chemical constituents of Codonopsis lanceolata, using the separation method of HP-20 macroporous resin column chromatography, Sephadex LH-20 chromatography for separation and purification of compounds of 50% ethanol elution fraction of Codonopsis lanceolata, structure of pure compounds obtained by the separation of 1 H-NMR and 13 C-NMR spectra and chemical identification methods. 6 compounds were isolated by repeated SephadexLH-20 column chromatography. 5 compounds were identified, namely, 5- hydroxymethyl -2- furan formaldehyde (1), CodonopilodiynosideA (2), lobetyol (3), (+) -isolariciresinol (4), and Codonopsis glucoside (5). The compounds (1), (2), (3) and (4) were all derived from the plant for the first time. The third part is the research on the quality control of Codonopsis lanceolata, using HPLC to establish a method for determination of Lobetyolin content, and collected a total of 13 batches of medicinal materials of Codonopsis lanceolata HPLC were measured using Chinese chromatogram, Pharmacopoeia Commission "chromatographic fingerprint similarity evaluation software A to evaluate the generated control map. This method is simple and quick, has the characteristics of strong specificity, stability, good repeatability, and can be used for quality control and evaluation of medicinal materials of Codonopsis lanceolata.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R284.1

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