【摘要】：當(dāng)今社會,食品安全問題受到廣泛的關(guān)注,而農(nóng)藥殘留問題是影響食品安全的一個重要因素,對人類健康和自然環(huán)境造成嚴(yán)重的影響。在各種農(nóng)藥殘留檢測技術(shù)中,經(jīng)典技術(shù)如實驗室用的大型氣相、液相色譜儀,雖然檢測精度高,但操作復(fù)雜,成本高昂,且難以實現(xiàn)現(xiàn)場快速檢測;而現(xiàn)有的快速檢測產(chǎn)品往往存在精度不高、配件眾多、操作繁瑣等問題。因此,發(fā)展快速、靈敏、便攜的有機磷農(nóng)藥檢測設(shè)備十分迫切。近年來,電化學(xué)生物傳感技術(shù)因其快速、靈敏、特異性強且具有微型化集成化的潛力而成為了生化檢測領(lǐng)域的重要發(fā)展方向。本論文以常用的有機磷農(nóng)藥為目標(biāo)物,設(shè)計和研究了便攜式有機磷農(nóng)藥檢測儀以及快速檢測酶生物傳感器。針對當(dāng)前農(nóng)藥殘留快速檢測的需求,論文首先提出了便攜有機磷農(nóng)藥殘留檢測的整體設(shè)計方案,包含酶生物傳感器、便攜式檢測儀的硬件與軟件的設(shè)計與調(diào)試,最終實現(xiàn)了對有機磷農(nóng)藥的高靈敏特異性檢測。在酶生物傳感器的設(shè)計及制作中,論文討論了絲印電極的制作、微通道的制作以及酶的固定;在硬件方面,本文依次討論了處理器控制電路、恒電位電路、微弱電流檢測電路、電壓掃描電路、采樣電路、濾波電路的設(shè)計;在軟件方面,本文介紹了微處理器程序和安卓端App的設(shè)計開發(fā)。最后利用精密電流源對設(shè)計的檢測儀進行了恒電位精度檢測和電流精度檢測,并對有機磷農(nóng)藥檢測儀的使用效果進行了驗證。結(jié)果表明恒電位電路可以控制電位的穩(wěn)定,誤差小于0.1 mV,電壓輸出范圍在-2.5 V到2.5 V之間。微電流檢測電路電流測量下限達(dá)到0.1 μA,酶生物傳感器對有機磷農(nóng)藥的檢測范圍在1 ng/mL-1000 ng/mL之間,檢測限達(dá)到有1 ng/mL。
[Abstract]:Nowadays, food safety has been paid more and more attention, and pesticide residue is an important factor affecting food safety, which has a serious impact on human health and natural environment. Among all kinds of pesticide residue detection techniques, classical techniques, such as large scale gas and liquid chromatography used in laboratory, have high precision, complex operation, high cost, and are difficult to realize rapid detection in the field. The existing rapid detection products often have problems such as low precision, numerous accessories, cumbersome operation and so on. Therefore, the development of rapid, sensitive, portable organophosphorus pesticide detection equipment is very urgent. In recent years, electrochemical biosensor technology has become an important development direction in the field of biochemical detection because of its rapid, sensitive, specific and miniaturization potential. In this paper, a portable organophosphorus pesticide detector and a rapid enzyme biosensor were designed and studied. In order to meet the need of rapid detection of pesticide residues, the whole design scheme of portable organophosphorus pesticide residue detection is proposed, including the design and debugging of the hardware and software of enzyme biosensor and portable detector. Finally, the high sensitive and specific detection of organophosphorus pesticides was realized. In the design and manufacture of enzyme biosensor, the fabrication of screen printing electrode, the fabrication of microchannel and the fixation of enzyme are discussed in this paper. In the aspect of hardware, the design of processor control circuit, constant potential circuit, weak current detection circuit, voltage scanning circuit, sampling circuit and filter circuit are discussed in turn. In the aspect of software, this paper introduces the design and development of microprocessor program and Android App. At last, the potentiostatic precision and current precision of the designed instrument are tested by using the precision current source, and the application effect of the organophosphorus pesticide detector is verified. The results show that the constant potential circuit can control the stability of the potential and the output error is less than 0. 1 mV, in the range of-2. 5 V to 2. 5 V. The detection limit of microcurrent detection circuit is 0. 1 渭 A, the detection range of enzyme biosensor for organophosphorus pesticide is between 1 ng/mL-1000 ng/mL and the detection limit is 1 ng/mL..
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TS207.53

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本文編號：2322381