本文選題：白芨多糖 + 超多孔水凝膠��； 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：白芨多糖(Bletilla striata polysaccharide,BSP)又稱為白芨膠、白芨甘露聚糖。其成分與魔芋葡甘聚糖相似,葡萄糖和甘露糖兩種糖基的摩爾比約為1:4,以甘露糖居多,由β-糖苷鍵聚合而成。作為一種水溶性植物β-葡聚糖,具有良好的親水性,分子中含有大量羥基可進(jìn)行化學(xué)改性。這些特性提示BSP可以應(yīng)用于水凝膠的制備。BSP具有抗氧化等多種生物學(xué)活性,作為止血藥、輔助抗癌藥物用于臨床。其食用安全,也作為添加劑或配料在食品工業(yè)領(lǐng)域使用。為改善BSP性能,拓寬其在醫(yī)藥領(lǐng)域的應(yīng)用,本研究以丙烯酸(AA)和丙烯酰胺(AM)為單體,在交聯(lián)劑N,N′-亞甲基雙丙烯酰胺(Bis)、引發(fā)劑過硫酸銨/N,N,N′,N′-四甲基乙二胺(APS/TEMED)、起泡劑NaHCO3、泡沫穩(wěn)定劑泊洛沙姆407/泊洛沙姆188[PF127:PF68=3:1(W/W)]等的作用下,采用泡沫聚合法制備半互穿網(wǎng)絡(luò)聚合物BSP超多孔水凝膠(BSP-SPH)。以溶脹比為指標(biāo),通過單因素實(shí)驗(yàn)優(yōu)化BSP-SPH的制備條件。利用傅里葉紅外光譜和核磁共振光譜考察其結(jié)構(gòu)變化,掃描電鏡觀察微觀形態(tài),并研究了不同介質(zhì)溶液對(duì)凝膠的溶脹性能及溶蝕性能的影響。實(shí)驗(yàn)結(jié)果:(1)BSP-SPH呈多孔狀,含有大量相互連通的孔隙,BSP分布在孔隙邊緣。(2)BSP的加入不僅可提高超多孔水凝膠的溶脹性能,溶脹比達(dá)到200倍,且改善了超多孔水凝膠體系的機(jī)械強(qiáng)度,密度較小,吸水充分溶脹后可懸浮,平均休止角為31.9±1.6°,流動(dòng)性較好,但極具引濕性,保存時(shí)要密閉保存;BSPSPH和SPH在不同介質(zhì)中的溶脹比從大到小依次為:蒸餾水pH 7.4 PBS0.1mol/L HCl。與SPH相比,BSP-SPH在蒸餾水及不同濃度NaCl和葡萄糖(Glucose)溶液中溶蝕率較低,BSP-SPH在不同pH值溶液中溶蝕率順序?yàn)?pH7.4 PBS0.1 mol/L HClpH 6.8 PBS。(3)以葛根素為模型藥物,MCC、淀粉、糊精及聚維酮K30等為輔料,采用濕法制粒的方法制備了葛根素分散片,并以分散均勻性為考察指標(biāo)對(duì)處方進(jìn)行篩選,單因素實(shí)驗(yàn)優(yōu)化出處方。以紫外分光光度法對(duì)分散片中葛根素含量及制劑體外累積溶出百分率進(jìn)行測(cè)定。所制得的分散片均能在3 min內(nèi)崩解,分散均勻性好。10 min體外累積溶出百分率為97.2%,初步評(píng)價(jià)了BSP改性材料對(duì)片劑崩解和溶出性能的影響。(4)采用MTT法初步測(cè)定了BSP-SPH的生物安全性。BSP-SPH濃度為10mg/mL時(shí),對(duì)細(xì)胞存活率無顯著影響(p0.05),初步表明BSP-SPH生物安全性較好。結(jié)論:本研究確定了BSP-SPH的制備工藝,制備的BSP-SPH溶脹、溶蝕性能得到改善,可作為崩解劑及親水性凝膠骨架材料應(yīng)用;BSP-SPH作為崩解劑制備的葛根素分散片,分散均勻性及體外溶出度均符合要求;體外研究初步表明BSP-SPH無明顯細(xì)胞毒性,生物相容性好。
[Abstract]:Bletilla striata polysaccharide (Bletilla striata polysaccharide) is also called bletilla striata gum. Its composition is similar to that of konjac glucomannan. The molar ratio of glucose to mannose is about 1: 4, which is mainly composed of 尾 -glucoside bond polymerization. As a water-soluble plant 尾 -glucan, it has good hydrophilicity and can be chemically modified with a large number of hydroxyl groups. These characteristics suggest that BSP can be used in the preparation of hydrogels. BSP has many biological activities such as antioxidation and so on. BSP can be used as a hemostatic and a supplementary anticancer drug for clinical use. Its edible safety, also used as additives or ingredients in the field of food industry. In order to improve the properties of (AA) and broaden its application in medicine, acrylic acid (AA) and acrylamide (AM) were used as monomers in this study. Under the action of the crosslinking agent N, N, N, N, N, N, N, N, N, N, N, N, N, N, P, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, N, The semi-interpenetrating polymer BSP superporous hydrogel (BSP-SPH) was prepared by foam polymerization. The preparation conditions of BSP-SPH were optimized by single factor experiment with swelling ratio as index. Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) were used to investigate the structural changes of the gel. The microstructure of the gel was observed by scanning electron microscope. The effects of different solutions on the swelling and corrosion properties of the gel were studied. The experimental results are as follows: (1) BSP-SPH is porous, with a large number of interconnected pores BSP distributed on the pore edge. (2) the addition of BSP can not only improve the swelling performance of the superporous hydrogel, the swelling ratio is 200 times, but also improve the mechanical strength of the superporous hydrogel system. The density is small, the average angle of repose is 31.9 鹵1.6 擄, the fluidity is good, but it is very hygroscopical. The swelling ratio of BSPSPH and SPH in different media should be closed. The order of swelling ratio of BSPSPH and SPH in different media is: distilled water pH 7.4 PBS0.1mol / L HCl. Compared with SPH, the dissolution rate of BSP-SPH in distilled water and different concentrations of NaCl and glucose (Glucose) solution was lower. The order of dissolution rate of BSP-SPH in different pH value solution was: 1: pH7.4 PBS0.1 mol / L HCl pH 6.8 PBS.The (3) puerarin was used as model drug MCC, starch, dextrin and polyvinylethone K30 as auxiliary materials. Puerarin dispersible tablets were prepared by wet granulation method. The content of puerarin in dispersible tablets and the cumulative dissolution percentage in vitro were determined by UV spectrophotometry. The dispersible tablets could be disintegrated in 3 min. The cumulative dissolution percentage of BSP-SPH was 97.2in vitro. The effects of BSP-modified materials on the disintegrating and dissolving properties of BSP-SPH were preliminarily evaluated. (4) when the concentration of BSP-SPH was 10 mg / mL, the biosafety of BSP-SPH was determined by MTT method, and the concentration of BSP-SPH was 10 mg / mL. There was no significant effect on cell survival rate (p0.05), which indicated that BSP-SPH was more safe than BSP-SPH. Conclusion: the preparation process of BSP-SPH was determined. The prepared BSP-SPH could be used as disintegrating agent and hydrophilic gel skeleton material to prepare puerarin dispersible tablets. The results of in vitro study showed that BSP-SPH had no obvious cytotoxicity and good biocompatibility.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TQ427.26

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