本文選題：羅丹明B + 拉曼散射��； 參考：《湖北工業(yè)大學》2017年碩士論文

【摘要】：隨著人民生活水平的提高,人們越來越關心食品的安全,食品安全始終是社會關注的重大問題,而食品中各種違法添加物是健康食品的重大威脅之一。拉曼散射檢測技術大多不接觸試樣,無需對試樣進行預處理,具有快速、準確的優(yōu)點。本文以違法添加物羅丹明B為研究對象,運用拉曼散射檢測技術實現(xiàn)了對羅丹明B拉曼圖譜的獲取和實驗數(shù)據處理,論文的主要內容如下:1、基于表面加強拉曼信號技術,對羅丹明B拉曼信號加強方法進行了實驗研究。對比了納米Ag增強劑和Au增強劑對羅丹明B拉曼信號的增強效果,以及Ag增強劑中匹配劑的作用。在此基礎上,探討了Ag增強劑,羅丹明B水溶液,匹配劑添加體積比例對拉曼信號增強效果的影響。結果表明,在785nm激光激發(fā)下,Ag增強劑對羅丹明B拉曼信號增強效果更佳,匹配劑的加入可顯著提高增強效果;當羅丹明B溶液濃度為0.7ppm時,當Ag增強劑:羅丹明B水溶液:匹配劑添加體積比例為1:4:1時,羅丹明B拉曼信號強度最大。2、在拉曼信號平滑去噪中,利用小波變換和窗口滑動平均法對羅丹明B拉曼信號進行了去噪,對比RSN和RMSE,小波變換硬閾值化法平滑去噪作用更好。在拉曼光譜基線消除中,在分段多項式擬合基線的基礎上,創(chuàng)新利用了分段三次樣條擬合基線的有效方法。三次樣條函數(shù)其特點是一、二階導數(shù)連續(xù)可導,則其刻畫出的曲線連接過渡平滑,用其擬合光譜基線,能改善信號的信噪比。通過對比最小二乘法擬合基線方法,其擬合出的基線不僅平滑而且準確,對羅丹明B的光譜基線消除效果更好。3、利用峰面積來計算拉曼特征峰光譜強度,并基于Wasson峰面積計算公式提出了累加峰強值法快速計算拉曼特征峰強度。針對拉曼光譜定量分析方法,結合外標法研究了多變量線性回歸方法,通過主成分線性回歸和偏最小二乘法線性回歸完成了對羅丹明B拉曼數(shù)據的簡單定量分析。
[Abstract]:With the improvement of people's living standards, people are more and more concerned about the safety of food. Food safety has always been a major concern of the society, and various illegal additives in food is one of the major threats to healthy food. Most Raman scattering techniques do not contact the sample and do not need to be pretreated. It has the advantages of rapidity and accuracy. In this paper, the illegal additive Rhodamine B is used as the research object, and the Raman spectrum of Rhodamine B is obtained and the experimental data are processed by Raman scattering technique. The main contents of this paper are as follows: 1, based on surface-enhanced Raman signal technology. The enhancement method of rodamine B Raman signal was studied experimentally. The effects of nano-Ag reinforcements and au reinforcements on the Raman signal enhancement of Rhodamine B and the matching agents in Ag reinforcements were compared. On this basis, the effects of Ag reinforcer, Rhodamine B aqueous solution and the proportion of matching agent on the enhancement of Raman signal were discussed. The results show that the enhancement effect of Ag on the Raman signal of Rhodamine B is better under the excitation of 785nm laser, and the enhancement effect can be improved by adding the matching agent, and when the concentration of Rhodamine B solution is 0.7ppm, the enhancement effect is better when the concentration of Rhodamine B solution is 0.7ppm. When Ag reinforcer: Rhodamine B aqueous solution: matching agent added volume ratio is 1:4:1, the intensity of Rhodamine B Raman signal is maximum. The rodamine B Raman signal is de-noised by wavelet transform and window moving average method. Compared with RSN and RMSE, wavelet transform hard threshold method is better for smooth denoising. On the basis of piecewise polynomial fitting baselines, the effective method of piecewise cubic spline fitting baselines is innovated in the elimination of Raman spectral baselines. The cubic spline function is characterized by continuous derivation of first and second derivative, then the curve is smooth and the signal to noise ratio can be improved by fitting the spectral baseline with the cubic spline function. By comparing the least square method to fit the baseline, the fitted baseline is not only smooth and accurate, but also can eliminate the spectral baseline of Rhodamine B. the spectral intensity of Raman characteristic peak is calculated by using peak area. Based on the formula of Wasson's peak area, the accumulative peak intensity method is proposed to calculate the Raman peak intensity quickly. In this paper, the multivariate linear regression method is studied based on the quantitative analysis of Raman spectroscopy and the external standard method. The simple quantitative analysis of rhodamine B Raman data is accomplished by principal component linear regression and partial least square linear regression.
【學位授予單位】：湖北工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TS207.3;O657.37

【參考文獻】

相關期刊論文 前10條

1 高嘉敏;張卓e，

本文編號：2028712