本文選題：載藥纖維 + 靜電紡絲技術(shù)�。� 參考：《天津工業(yè)大學(xué)》2017年碩士論文

【摘要】：載藥納米纖維是一種被廣泛利用于靶細(xì)胞運(yùn)輸和釋放的藥物載體,在給藥過(guò)程中使藥物緩慢釋放,因此備受關(guān)注。本文通過(guò)靜電紡絲技術(shù)制備了膠原蛋白納米纖維膜,分別用EDC/NHS和檸檬酸交聯(lián)體系對(duì)其進(jìn)行交聯(lián)改性,并對(duì)交聯(lián)前后的納米纖維膜進(jìn)行了結(jié)構(gòu)和性能研究。通過(guò)靜電紡絲技術(shù),在電壓20 kv、接收距離15 cm、擠出速率0.8 m1/h、紡絲溶液濃度9%的紡絲條件下,制備出的納米纖維光滑,并且直徑分布均勻。EDC/NHS和檸檬酸交聯(lián)體系交聯(lián)后的納米纖維,通過(guò)FSEM觀察到了明顯的交聯(lián)網(wǎng)絡(luò)存在。通過(guò)FT-IR對(duì)比發(fā)現(xiàn),交聯(lián)處理并未改變膠原蛋白原有的三螺旋結(jié)構(gòu),因此不會(huì)改變膠原蛋白的優(yōu)異性能。通過(guò)對(duì)比交聯(lián)前后的水接觸角,發(fā)現(xiàn)交聯(lián)后的納米纖維的親水性并未發(fā)生改變。用考馬斯亮藍(lán)法結(jié)合紫外分光光度計(jì)對(duì)交聯(lián)前后納米纖維的降解行為和載藥前后的藥物釋放行為進(jìn)行了表征和對(duì)比。交聯(lián)前的納米纖維降解時(shí)間為10天,交聯(lián)后的納米纖維降解時(shí)間提高到了 21天,耐水性也有顯著的提高。在最佳紡絲條件下,制備了載藥膠原蛋白納米纖維膜,采用FSEM和TEM對(duì)纖維形貌及內(nèi)部結(jié)構(gòu)進(jìn)行了表征。結(jié)果表明,在納米纖維表面和內(nèi)部都觀察不到5-FU藥物顆粒的存在,說(shuō)明5-FU與膠原蛋白的相容性極好,分子級(jí)均勻分布于膠原蛋白纖維中。通過(guò)研究交聯(lián)前后的載藥納米纖維藥物釋放,發(fā)現(xiàn)交聯(lián)后的載藥纖維的藥物突釋現(xiàn)象得到了明顯的降低,藥物利用率提高了 10%-30%。采用這種交聯(lián)后的納米纖維進(jìn)行給藥,能夠減輕患者的痛苦和不便,是一種很有前景的給藥方式。
[Abstract]:Drug-loaded nanofibers are widely used as drug carriers for the transport and release of target cells. In this paper, collagen nanofilament membranes were prepared by electrospinning technique and were crosslinked by EDC / NHS and citric acid crosslinking system respectively. The structure and properties of the nanofibers before and after crosslinking were studied. Under the conditions of 20 kv voltage, 15 cm receiving distance, 0.8 ml / h extrusion rate and 9% concentration of spinning solution, the nanofibers prepared by electrospinning technology are smooth, and the diameter distribution is even. EDC / NHS is crosslinked with citric acid crosslinking system. The existence of cross-linking network was observed by FSEM. By FT-IR comparison, it was found that the crosslinking treatment did not change the original triple helix structure of collagen, so it would not change the excellent performance of collagen. By comparing the water contact angle before and after crosslinking, it is found that the hydrophilicity of the crosslinked nanofibers has not changed. Coomassie brilliant blue method and UV spectrophotometer were used to characterize and compare the degradation behavior and drug release behavior of nanofibers before and after crosslinking. The degradation time of the nanofibers before crosslinking was 10 days, and the degradation time of the cross-linked nanofibers increased to 21 days, and the water resistance was also significantly improved. Under the optimum spinning conditions, the drug-loaded collagen nanofibers were prepared, and the morphology and internal structure of the fibers were characterized by FSEM and TEM. The results showed that the presence of 5-FU drug particles was not observed on the surface and inside of nanofibers, indicating that 5-FU had excellent compatibility with collagen, and was evenly distributed in collagen fibers at molecular level. By studying the drug release of drug-loaded nanofibers before and after crosslinking, it was found that the drug sudden release of the cross-linked fibers was obviously reduced, and the drug utilization rate was increased by 10-30%. This cross-linked nanofiber can alleviate the pain and inconvenience of patients and is a promising drug delivery method.
【學(xué)位授予單位】：天津工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TQ340.64;TQ460.4

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