發(fā)布時(shí)間：2018-05-15 21:31

  本文選題：碳點(diǎn) + 熒光探針�。� 參考：《天津工業(yè)大學(xué)》2017年碩士論文

【摘要】：熒光碳點(diǎn)是一類(lèi)粒徑小于10 nm的新型納米材料。由于具有小尺寸、卓越的水溶性、好的生物相容性、低細(xì)胞毒性、高的光穩(wěn)定性、可調(diào)的激發(fā)和發(fā)射行為及表面易于功能化,使其廣泛應(yīng)用在眾多領(lǐng)域,如分析檢測(cè)、生物成像、光催化等。本文分別采用水熱法、煅燒法及回流法合成了一系列碳點(diǎn),主要研究了其發(fā)光性能,并將其應(yīng)用于生物成像、分析檢測(cè)和光催化劑的設(shè)計(jì)。論文的主要內(nèi)容包括以下幾部分:1.本實(shí)驗(yàn)首先用水熱法制備了熒光碳點(diǎn):以已二酸和檸檬酸銨制備的CD1能夠作為Hg2+的熒光探針,通過(guò)實(shí)驗(yàn)發(fā)現(xiàn)CD1在4-18 μ的線性范圍內(nèi)可以實(shí)現(xiàn)定量檢測(cè),在細(xì)胞中通過(guò)觀察熒光的變化可以識(shí)別對(duì)其Hg2+進(jìn)行分析;以卡波姆934與二乙烯三胺為原料制得CD2,Co2+與碳點(diǎn)通過(guò)金屬配鍵作用使其溶液發(fā)生肉眼可見(jiàn)的顏色變化并且熒光猝滅,在0-40 μ線性范圍內(nèi)可以定量檢測(cè),并且可以應(yīng)用于實(shí)際水樣中Co2+的分析檢測(cè);通過(guò)無(wú)水檸檬酸和4,7,10-三氧-1,13-十三烷二胺制備的CD3與多巴胺通過(guò)靜電作用螯合熒光猝滅,而隨著Al3+或者谷胱甘肽(GSH)的加入,可以從螯合物中奪取多巴胺釋放碳點(diǎn)使其熒光恢復(fù),另外該探針還可以在生物體液中檢測(cè)多巴胺,在食物樣品中檢測(cè)Al3+和人體血清中檢測(cè)到GSH。2.然后用煅燒法合成了疏水性碳點(diǎn)CD4,通過(guò)實(shí)驗(yàn)證明得到的碳點(diǎn)能夠在有機(jī)溶劑中很好的溶解,并且在乙醇中能夠選擇性地快捷靈敏的檢測(cè)Hg2+;進(jìn)一步通過(guò)CTAB修飾制得兩親性CD5,構(gòu)建了基于熒光共振能量轉(zhuǎn)移(FRET)機(jī)理的熒光檢測(cè)體系,實(shí)現(xiàn)表明CD5能夠作為有效的能量供體,將其能量轉(zhuǎn)移給受體桑色素-Al3+,實(shí)現(xiàn)對(duì)Al3+的定性及定量分析,另外該體系由于細(xì)胞無(wú)毒性可應(yīng)用于細(xì)胞中Al3+的識(shí)別與成像。3.設(shè)計(jì)了一步回流法制備CD6,實(shí)驗(yàn)發(fā)現(xiàn)該碳點(diǎn)不僅具有下轉(zhuǎn)換熒光性質(zhì),而且還具有優(yōu)秀的上轉(zhuǎn)換熒光性質(zhì),建立了一種操作簡(jiǎn)單的多功能熒光檢測(cè)體系。當(dāng)以下轉(zhuǎn)換為激發(fā)源時(shí),CD6能夠高選擇性地檢測(cè)三硝基苯酚(TNP),在0-50 μM線性范圍內(nèi)定量檢測(cè);當(dāng)以上轉(zhuǎn)換為激發(fā)源時(shí),TNP不能使其熒光發(fā)生猝滅,而Fe3+使其熒光增強(qiáng),在8-40 μM濃度范圍內(nèi),熒光變化與Fe3+呈現(xiàn)很好的線性關(guān)系可以實(shí)現(xiàn)定量檢測(cè)。4.合成了基于碳點(diǎn)的光催化劑,碳點(diǎn)優(yōu)異的熒光性質(zhì)能夠拓寬催化劑可利用光的范圍,提高催化效率。通過(guò)在WO3表面修飾碳點(diǎn),得到的CDs/WO3在可見(jiàn)光照射下具有很強(qiáng)的光的吸收。相比于純WO3和CDs,該復(fù)合物具有較高的光催化活性,經(jīng)0.5小時(shí)光照后,亞甲基藍(lán)的降解率可達(dá)到100%;另外用CD6修飾氮化碳(C3N4),得到的復(fù)合物CD6/C3N4在可見(jiàn)光照射下其催化效果高于純C3N4和CD6。最后解釋了這兩種光催化劑具有高效催化活性的機(jī)理。
[Abstract]:Fluorescent carbon dots are a new kind of nanomaterials with particle size less than 10 nm. Due to its small size, excellent water solubility, good biocompatibility, low cytotoxicity, high photostability, adjustable excitation and emission behavior and easy surface functionalization, it is widely used in many fields, such as analytical detection, Biological imaging, photocatalysis, etc. In this paper, a series of carbon spots were synthesized by hydrothermal method, calcination method and reflux method, respectively. The luminescent properties of carbon spots were studied and applied to biometric imaging, analysis and detection, and the design of photocatalyst. The main contents of the thesis include the following parts: 1. In this experiment, the fluorescent carbon points were prepared by hydrothermal method. CD1 prepared by adipic acid and ammonium citrate could be used as a fluorescence probe for Hg2. It was found that CD1 could be quantitatively detected in a linear range of 4-18 渭. The Hg2 can be identified and analyzed by observing the changes of fluorescence in the cells, and the color change of the solution can be seen by metal bond and quenching of fluorescence, which is made from Carbomer 934 and diethylenetriamine. It can be quantitatively detected in the range of 0-40 渭, and can be applied to the analysis and detection of Co2 in real water samples. CD3 prepared from anhydrous citric acid and 4o 710- trioxy-1-tridecanediamine and dopamine can be chelated to fluorescence quenching by electrostatic interaction. With the addition of Al3 or glutathione (GSH), dopamine releasing carbon can be captured from the chelate to recover its fluorescence. In addition, the probe can detect dopamine in biological fluid, Al3 in food samples and GSH.2in human serum. Then the hydrophobic carbon spot CD4 was synthesized by calcination method. The experimental results show that the carbon spots can be dissolved in organic solvents and can be used to detect Hg2 quickly and selectively in ethanol. Furthermore, the amphiphilic CD5 was prepared by CTAB modification, and a fluorescence detection system based on fluorescence resonance energy transfer (fret) mechanism was constructed. The results show that CD5 can act as an effective energy donor and transfer its energy to the receptor Morin Al3. The qualitative and quantitative analysis of Al3 can be realized. In addition, the system can be applied to the recognition and imaging of Al3 in cells because of its nontoxicity. A one-step reflux method was designed to prepare CD6. It was found that the carbon point had not only downconversion fluorescence properties but also excellent up-conversion fluorescence properties. A multifunctional fluorescence detection system with simple operation was established. When the following conversion is an excitation source, TNP6 can be detected with high selectivity and quantificationally in the linear range of 0-50 渭 M. when the above conversion is an excitation source, it can not quench its fluorescence, while Fe3 enhances its fluorescence. In the range of 8-40 渭 M, the linear relationship between fluorescence change and Fe3 can be achieved. The photocatalyst based on carbon point was synthesized. The excellent fluorescence property of carbon point can widen the range of light available and improve the catalytic efficiency. By modifying the carbon point on the surface of WO3, the CDs/WO3 has strong light absorption under visible light irradiation. Compared with pure WO3 and CDS, the complex has higher photocatalytic activity, and the degradation rate of methylene blue can reach 100 after 0.5 hours of illumination, and the CD6/C3N4 modified with CD6 is more effective than that of pure C3N4 and CD6 under visible light irradiation. Finally, the mechanism of high activity of these two photocatalysts was explained.
【學(xué)位授予單位】：天津工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O657.3

【參考文獻(xiàn)】

中國(guó)期刊全文數(shù)據(jù)庫(kù) 前1條

1 鄒麗霞;鐘秦;劉慶成;;Preparation and Characterization of Microporous Nano-Tungsten Trioxide and Its Photocatalytic Activity after Doping Rare Earth[J];Journal of Rare Earths;2006年01期

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本文編號(hào)：1894041