發(fā)布時(shí)間：2019-03-26 15:41

【摘要】：全球土壤鹽漬化日益嚴(yán)重,我國鹽漬土面積已顯著高于全球平均水平。鹽脅迫嚴(yán)重影響植物生長發(fā)育,鹽生植物與鹽敏感植物相比在應(yīng)答鹽脅迫的過程中可能存在獨(dú)特的調(diào)控機(jī)制,本研究利用耐鹽植物喜鹽鸞尾(Iris.halophila Pall.)作為研究對(duì)象來探討其耐鹽機(jī)理。氫氣(Hydrogen,H2)作為一種新型調(diào)節(jié)植物生理過程中的信號(hào)分子,可通過影響植物激素、調(diào)節(jié)離子平衡的轉(zhuǎn)運(yùn)蛋白及其轉(zhuǎn)錄因子、抗氧化酶、根際微生物等參與和調(diào)節(jié)植物生長發(fā)育、成熟衰老、脅迫耐受等多種生理過程。本研究對(duì)H2是否可以增強(qiáng)喜鹽鳶尾鹽耐受性展開研究,以期為進(jìn)一步提升耐鹽植物耐受性提供新的思路。主要研究結(jié)果如下:1.喜鹽鳶尾受到鹽脅迫后通過提高抗氧化酶活性、可溶性蛋白含量及控制Na+轉(zhuǎn)運(yùn)相關(guān)基因以減緩鹽處理對(duì)愈傷組織氧化損傷程度。2.用 50、100、500mmol/L NaCl 及 25%、50%、100%富氫水(Hydrogen-rich water,HRW)混合溶液處理喜鹽鳶尾愈傷組織,結(jié)果顯示25%、50%、100%HRW可不同程度緩解鹽脅迫對(duì)喜鹽鳶尾造成的損傷,其中100%HRW效果最佳。3.HRW處理可提高抗氧化酶(SOD、POD、可溶性蛋白)的活性,減緩鹽處理對(duì)愈傷組織氧化損傷程度。4.HRW處理可顯著提高喜鹽鳶尾NHX基因的表達(dá)量,降低鈉鉀離子比,增強(qiáng)喜鹽鳶尾耐鹽性。5.HRW處理可提高喜鹽鳶尾內(nèi)源NO含量,而NO已被證明可參與到植物耐鹽調(diào)控中。本研究采用生理生化、化學(xué)及分子手段對(duì)HRW的保護(hù)作用進(jìn)行探究,研究結(jié)果表明HRW處理喜鹽鳶尾可提高其抗氧化酶活性,增加NHX基因表達(dá)量,降低鈉鉀離子比,提高內(nèi)源NO含量,說明HRW可能通過調(diào)節(jié)Na+轉(zhuǎn)運(yùn)及提高抗氧化活性等方式減輕鹽脅迫損傷,這一結(jié)果為進(jìn)一步探究氫氣的脅迫保護(hù)作用提供了堅(jiān)實(shí)基礎(chǔ)。
[Abstract]:The salinization of the global soil is becoming more and more serious, and the area of saline soil in China has been significantly higher than that of the global average. Salt stress seriously affects the growth and development of the plant, and the salt-sensitive plant may have a unique control mechanism in response to salt stress in response to salt stress. ) The salt-tolerant mechanism of the study was discussed. hydrogen (HEMAgen, H2) is used as a signal molecule in a novel regulation plant physiological process, and can participate in and regulate the growth and development of a plant by influencing the plant hormone, regulating the transport protein of the ion balance and the transcription factor, the antioxidant enzyme, the rhizosphere microorganism, and the like, Stress tolerance and other physiological processes. In order to provide a new way to further improve the tolerance of salt-tolerant plants, the study was conducted to investigate whether H2 could enhance the tolerance of salt-tolerant plants. The main results are as follows:1. The effect of salt stress on the activity of antioxidant enzymes, the content of soluble protein and the control of Na + transport-related genes to reduce the degree of oxidative damage to the callus after salt stress. The results showed that 50%,100,500 mmol/ L NaCl and 25%,50%,100% hydrogen-rich water (HRW) were used to treat the callus of the iris. The results showed that 25%,50%,100% HRW could alleviate the damage to the iris of the iris by salt stress, and the effect of 100% HRW was the best.3. The treatment of HRW could improve the antioxidant enzymes (SOD, POD, 4. The treatment of HRW could increase the content of endogenous NO in the iris of the iris. NO has been shown to be involved in plant salt tolerance regulation. In this study, the effects of physiological, biochemical, chemical and molecular mechanisms on the protection of HRW were studied. The results showed that HRW could increase the activity of antioxidant enzymes, increase the expression of NHX, decrease the ratio of sodium and potassium ions, and improve the content of endogenous NO. It is suggested that HRW can reduce the salt stress damage by adjusting Na + transport and improving the anti-oxidation activity. The result provides a solid foundation for further exploring the stress protection of hydrogen.
【學(xué)位授予單位】：中央民族大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q945.78

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