發(fā)布時(shí)間：2018-10-31 16:23

【摘要】：傳統(tǒng)的微重力研究只能在空間站、失重飛機(jī)、落塔等空間設(shè)備上進(jìn)行,但均難以避免模擬時(shí)間短,成本高的代價(jià)。旋轉(zhuǎn)式反應(yīng)器模擬微重力效應(yīng)原理與空間失重效應(yīng)類似,因此被國際上微重力實(shí)驗(yàn)室所采用,甚至醫(yī)學(xué)、遺傳學(xué)、生物學(xué)等領(lǐng)域?qū)＜覍W(xué)者運(yùn)用該設(shè)備來觀察微生物、動(dòng)植物體的微重力應(yīng)激反應(yīng)。現(xiàn)有的旋轉(zhuǎn)式生物反應(yīng)器都面臨實(shí)現(xiàn)培養(yǎng)液供給和控制流動(dòng)剪切力之間矛盾問題,培養(yǎng)液供給不可避免地帶來了剪切力作用,剪切力作用不利于植物生長。本課題來源于福建省農(nóng)科院生態(tài)研究所承擔(dān)的國家高技術(shù)研究發(fā)展項(xiàng)目(2010AA743042),福建省自然科學(xué)基金項(xiàng)目(2011J01101)。以三維旋轉(zhuǎn)生物反應(yīng)器為研究對(duì)象,立足于培養(yǎng)液供給和流動(dòng)剪切力這2個(gè)基點(diǎn)出發(fā),研制一種能夠模擬植物微重力效應(yīng)三維旋轉(zhuǎn)反應(yīng)器,本文主要完成如下工作：1)運(yùn)用功能分析法對(duì)三維旋轉(zhuǎn)反應(yīng)器進(jìn)行功能元分解和形態(tài)學(xué)矩陣重組設(shè)計(jì),得出多種解法并選取最優(yōu)解；2)測(cè)量304不銹鋼內(nèi)壁實(shí)驗(yàn)組與對(duì)照組2種條件下栽培盤的光照強(qiáng)度,驗(yàn)證304不銹鋼內(nèi)壁能否滿足植物光源需求,此外,運(yùn)用ANSYS對(duì)旋轉(zhuǎn)軸進(jìn)行結(jié)構(gòu)分析；3)應(yīng)用FLUENT軟件對(duì)二維、三維旋轉(zhuǎn)生物反應(yīng)器栽培盤流體分析,比較二維、三維旋轉(zhuǎn)生物反應(yīng)器水培植物的剪切力整體水平對(duì)比,結(jié)果表明,三維旋轉(zhuǎn)反應(yīng)器較小的剪切力水平更適合栽培植物,研究離軸位置與流體剪切力關(guān)系,得出結(jié)論,離軸距離越近,剪切力峰值越小。樣機(jī)完成后應(yīng)用于多種植物體(紅萍、莧菜)進(jìn)行旋轉(zhuǎn)培育實(shí)驗(yàn),實(shí)驗(yàn)結(jié)論表明：說明三維旋轉(zhuǎn)生物反應(yīng)器對(duì)紅萍根系生長有著顯著影響；此外,莧菜株高實(shí)驗(yàn)結(jié)果表明,栽培位置X軸為基準(zhǔn)旋轉(zhuǎn)軸,離軸距離越小,幼苗的株高在同等培育條件下略高與對(duì)照,這種方式宏觀上亦驗(yàn)證了FLUENT流體分析的剪切力理論。
[Abstract]:Traditional microgravity research can only be carried out on space equipment such as space station, weightlessness aircraft and landing tower, but it is difficult to avoid the cost of short simulation time and high cost. The principle of simulating microgravity effects in rotary reactors is similar to that of space weightlessness, so it has been used in microgravity laboratories around the world, and even experts and scholars in medicine, genetics, biology and other fields have used this equipment to observe microbes. The microgravity stress response of animals and plants. The existing rotary bioreactor is confronted with the contradiction between the supply of culture medium and the control of flow shear force, which inevitably brings about shear force, which is not conducive to plant growth. This project comes from the National High Technology Research and Development Project (2010AA743042) and the Natural Science Foundation (2011J01101) of Fujian Provincial Academy of Agricultural Sciences. A three-dimensional rotating bioreactor was developed based on the two basis points of medium supply and flow shear force, which can simulate the microgravity effect of plants. The main work of this paper is as follows: 1) the functional element decomposition and morphological matrix reorganization design of 3D rotating reactor are carried out by using functional analysis method, and various solutions are obtained and the optimal solution is selected; 2) measuring the light intensity of the inner wall of 304 stainless steel under two kinds of conditions: experimental group and control group, to verify whether the inner wall of 304 stainless steel can meet the demand of plant light source. In addition, ANSYS was used to analyze the structure of rotation shaft; 3) FLUENT software was used to analyze the fluid of two-dimensional and three-dimensional rotating bioreactor, and to compare the shear stress of hydroponic plants in two-dimensional and three-dimensional rotating bioreactor. The results showed that: 1. The relationship between the off-axis position and the fluid shear force is studied. It is concluded that the closer the distance from the axis to the axis, the smaller the peak value of shear force. After the prototype was finished, it was applied to a variety of plant (red duckweed, amaranth) for rotation cultivation experiment. The experimental results showed that the three-dimensional rotating bioreactor had a significant effect on the root growth of red duckweed. In addition, the experimental results of plant height of Amaranth showed that the plant height of seedlings was slightly higher than that of the control under the same cultivation conditions. The experimental results showed that the cultivation position X axis was the base rotation axis and the distance from the axis was smaller. This method also verified the shear force theory of FLUENT fluid analysis.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：Q94-337

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