本文選題：WRKY + DoWRKY6��； 參考：《西南交通大學(xué)》2017年碩士論文

【摘要】：WRKY是植物轉(zhuǎn)錄因子家族中較為重要的一員,在植物的生長(zhǎng)發(fā)育過(guò)程中起著極為重要的轉(zhuǎn)錄調(diào)控作用。本論文基于鐵皮石斛原球莖轉(zhuǎn)錄組數(shù)據(jù),獲得了 20個(gè)DoWRKY轉(zhuǎn)錄因子家族成員的保守區(qū)片段,并進(jìn)一步研究了DoWRKY 基因在不同齡級(jí)、莖不同部位及實(shí)驗(yàn)室組培幼苗的鐵皮石斛材料中的表達(dá)情況。通過(guò)對(duì)20個(gè)DoWRKY基因的保守區(qū)序列進(jìn)行系統(tǒng)發(fā)育與序列保守性分析,發(fā)現(xiàn)這些成員都,有至少一個(gè)WRKYGQK構(gòu)成的氨基酸殘基保守結(jié)構(gòu)域,并且通過(guò)系統(tǒng)發(fā)育樹的構(gòu)建,發(fā)現(xiàn)這20個(gè)基因親緣性有較大差異。通過(guò)生物信息學(xué)分析獲得了鐵皮石斛DoWRKY6基因的蛋白序列特征,鐵皮石斛DoWRKY6基因具有長(zhǎng)度為1,017bp的完整ORF,編碼339個(gè)氨基酸,該蛋白只有一個(gè)WRKY結(jié)構(gòu)域和一個(gè)結(jié)構(gòu)為CX5C23HX1H的鋅指結(jié)構(gòu),屬于II類WRKY轉(zhuǎn)錄因子,且WRKY 結(jié)構(gòu)域與鋅指結(jié)構(gòu)會(huì)和DNA的W盒進(jìn)行特異性結(jié)合。通過(guò)Protparam在線預(yù)測(cè)分析DoWRKY6蛋白的分子式為C1641H2556N454O530S13,相對(duì)分子質(zhì)量為37,541.8,理論等電點(diǎn)為 4.86,總平均親水性(Grand average of hydropathicity,GRAVY)為-0.756,判定DoWRKY6灰轉(zhuǎn)錄因子為疏水性蛋白。利用熒光實(shí)時(shí)定量PCR技術(shù)獲得了DoWRKY6基因在不同齡級(jí)及莖不同部位的鐵皮石斛中的表達(dá)特點(diǎn)和在組培幼苗的不同器官中的相對(duì)表達(dá)量,發(fā)現(xiàn)DoWRKY6基因主要在莖中表達(dá),且隨著生長(zhǎng)年限增長(zhǎng)其表達(dá)量會(huì)大大降低;而在莖中又主要是在莖的芽端部分表達(dá),其次是在莖的基部表達(dá),從而表明DoWRKY6對(duì)鐵皮石斛莖的生長(zhǎng)發(fā)育有重要的調(diào)控作用。
[Abstract]:WRKY is an important member of the family of plant transcription factors, which plays an important role in the process of plant growth and development. Based on the transcriptome data of protocorm of Dendrobium candidum, the conservative regions of 20 members of DoWRKY transcription factor family were obtained, and the DoWRKY gene was further studied at different age levels. Expression of Dendrobium candidum in different parts of stem and tissue culture seedlings in laboratory. Based on the phylogenetic and conserved analysis of 20 conserved regions of DoWRKY gene, it was found that all of these members had at least one amino acid residues conserved domain of WRKYGQK, and the phylogenetic tree was constructed. It was found that there were significant differences in the genetic relationship of the 20 genes. The protein sequence of Dendrobium candidum DoWRKY6 gene was obtained by bioinformatics analysis. Dendrobium candidum DoWRKY6 gene has a complete ORFwhose length is 1017bp and encodes 339 amino acids. The protein has only one WRKY domain and a zinc finger structure with CX5C23HX1H structure. It belongs to class II WRKY transcription factors, and the WRKY domain binds specifically to the zinc finger structure and the W box of DNA. The molecular formula of DoWRKY6 protein was C1641H2556N454O530S13, the relative molecular weight was 37541.8, the theoretical isoelectric point was 4.86, and the total average hydrophobic average of hydropathicity GRAVYwas -0.756. The grey transcription factor of DoWRKY6 was determined as hydrophobic protein. The expression characteristics of DoWRKY6 gene in Dendrobium candidum at different age levels and different parts of stem were obtained by real-time quantitative PCR. The results showed that DoWRKY6 gene was mainly expressed in stems of Dendrobium candidum in different organs of tissue culture seedlings. The expression of DoWRKY6 was mainly expressed at the end of shoot and then at the base of stem, which indicated that DoWRKY6 played an important role in the growth and development of Dendrobium candidum stem.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q943.2;S567.239

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周良云;劉談;王升;唐金富;康利平;郭蘭萍;;實(shí)時(shí)熒光定量PCR研究進(jìn)展及其在中藥領(lǐng)域的應(yīng)用[J];中國(guó)現(xiàn)代中藥;2016年02期

2 Xiu Tian;Juxin Ruan;Jinquan Huang;Xin Fang;Yingbo Mao;Lingjian Wang;Xiaoya Chen;Changqing Yang;;Gossypol: phytoalexin of cotton[J];Science China(Life Sciences);2016年02期

3 蔣園;朱玉球;高燕會(huì);斯金平;;鐵皮石斛WRKY5基因的克隆與表達(dá)分析[J];中草藥;2016年02期

4 徐群剛;鄺健飛;單偉;陸旺金;陳建業(yè);;香蕉果實(shí)冷脅迫相關(guān)MaWRKY11轉(zhuǎn)錄因子的特性、互作蛋白篩選與鑒定[J];熱帶亞熱帶植物學(xué)報(bào);2015年05期

5 趙俊;孟燦燦;趙亦帆;王晨曦;樊洪泓;金青;李大輝;林毅;蔡永萍;;鐵皮石斛轉(zhuǎn)錄因子基因(DoWRKY2)的克隆與表達(dá)分析[J];農(nóng)業(yè)生物技術(shù)學(xué)報(bào);2015年08期

6 趙俊;孫時(shí)偉;孟燦燦;金青;樊洪泓;林毅;蔡永萍;;鐵皮石斛轉(zhuǎn)錄因子基因DoWRKY1的克隆與表達(dá)分析[J];中國(guó)中藥雜志;2015年14期

7 JIANG YanJuan;YU DiQiu;;WRKY transcription factors: links between phytohormones and plant processes[J];Science China(Life Sciences);2015年05期

8 唐麗;李菁;龍華;李貴;;不同生長(zhǎng)齡鐵皮石斛莖與葉中總多糖、總生物堿及總黃酮含量的差異[J];廣東農(nóng)業(yè)科學(xué);2015年08期

9 WU Xiao-jun;Xu Li;ZHAO Pan-feng;LI Na;WU Lei;HE Yan;WANG Shou-cai;;Comparative transcriptome profiling of two maize near-isogenic lines differing in the allelic state for bacterial brown spot disease resistance[J];Journal of Integrative Agriculture;2015年04期

10 王佳;鄭琳琳;顧天培;王學(xué)峰;王迎春;;珍稀泌鹽植物長(zhǎng)葉紅砂兩個(gè)WRKY轉(zhuǎn)錄因子的克隆及表達(dá)分析[J];草業(yè)學(xué)報(bào);2014年04期

相關(guān)會(huì)議論文 前1條

1 張曉楓;姜濤;王小芳;張大鵬;;ABA信號(hào)轉(zhuǎn)導(dǎo):ADR2拮抗ABAR-WRKY40信號(hào)通路[A];2011全國(guó)植物生物學(xué)研討會(huì)論文集[C];2011年

相關(guān)博士學(xué)位論文 前2條

1 包英華;鐵皮石斛種質(zhì)資源的鑒定與評(píng)價(jià)研究[D];廣州中醫(yī)藥大學(xué);2014年

2 周杰;活性氧、激素互作、自噬和轉(zhuǎn)錄因子在番茄和擬南芥逆境脅迫響應(yīng)中的作用機(jī)理和調(diào)控[D];浙江大學(xué);2014年

相關(guān)碩士學(xué)位論文 前4條

1 馮沛春;山慈菇（杜鵑蘭）快速繁殖技術(shù)優(yōu)化及鐵皮石斛葉cDNA PCR文庫(kù)的構(gòu)建[D];西南交通大學(xué);2014年

2 朱乾坤;AtNAC1轉(zhuǎn)錄因子的分子建模和鐵皮石斛NAC家族基因的克隆及表達(dá)分析[D];西南交通大學(xué);2014年

3 丁懿;NAC家族對(duì)鐵皮石斛原球莖發(fā)生的機(jī)理研究與PIN的生物信息學(xué)分析[D];西南交通大學(xué);2012年

4 黃佳玲;葡萄WRKY基因的克隆和功能分析[D];遼寧師范大學(xué);2012年

，

本文編號(hào)：1997285