本文選題：甜瓜 + MADS-box基因　； 參考：《內(nèi)蒙古大學》2017年碩士論文

【摘要】：甜瓜(Cucumis melo L.)是一年生雙子葉的草本蔓生植物,也是一種重要的園藝作物和營養(yǎng)豐富的水果,被廣泛種植于世界各地并譽為世界十大水果之一。甜瓜是典型的雄花和兩性花同體植物,其花器官發(fā)育明顯符合ABC(D)E發(fā)育模式。本實驗所用材料是典型的呼吸躍變型果實,是研究鮮果果實發(fā)育成熟的理想對象。MADS-box基因家族編碼一類轉(zhuǎn)錄因子,對植物生長發(fā)育,尤其是對生殖器官發(fā)育的調(diào)控起著重要的作用,是花發(fā)育及果實成熟的重要調(diào)控因子。本論文以甜瓜品種河套蜜瓜(Cucumis melo L.cv.Hetao)為實驗材料,應用生物信息學的方法從其基因組中鑒定篩選出MADS-box基因家族的全部成員,共鑒定得到62個甜瓜MADS-box基因,其中26個屬于Ⅰ型基因,分布在Mα、Mγ和Mδ亞家族中;36個屬于Ⅱ型基因,分布在MIKCc型各亞家族中。其中,在Mβ、AGL12、BS、TM8和MIKC*亞家族中沒有任何基因的分布。同時對該家族所有基因進行了染色體定位、內(nèi)含子-外顯子結(jié)構(gòu)、保守基序分布、系統(tǒng)發(fā)生與進化關(guān)系分析,結(jié)果顯示,大部分成員的特性均與其所在亞家族的其他成員相一致,這也說明在不同物種間,MADS-box家族基因在結(jié)構(gòu)和進化上是相對保守的。應用半定量方法對甜瓜MADS-box基因家族各成員進行了表達分析,根據(jù)檢測結(jié)果,從每個亞家族選取1～2個表達量較顯著且表達模式有特點的基因,采用實時熒光定量法進一步對其表達特性進行檢測,結(jié)果顯示,不同亞家族基因呈現(xiàn)出不同的表達模式,預示著其廣泛的生物學功能。應用RT-PCR法克隆了CmMADS02和CmMADS26基因全長cDNA,并構(gòu)建其超表達和RNAi載體。應用瞬時表達技術(shù)分析其在甜瓜果實成熟中的作用,結(jié)果顯示CmMADS26基因?qū)μ鸸瞎麑嵆墒炜赡芫哂写龠M作用。對轉(zhuǎn)CmMADS02基因的T1代甜瓜植株表型分析顯示,CmMADS02基因可能參與調(diào)控甜瓜兩性花雄蕊的發(fā)育。
[Abstract]:Cucumis melo L. It is an annual dicotyledonous herbaceous vine. It is also an important horticultural crop and nutritious fruit. It is widely planted all over the world and is regarded as one of the top ten fruits in the world. Muskmelon is a typical male flower and hermaphrodite plant, its floral organ development obviously accords with ABC(D)E development pattern. The material used in this study is a typical respiratory variant fruit. It is an ideal target for studying the development and maturation of fresh fruit. MADS-box gene family encodes a class of transcription factors, which is useful for the growth and development of plants. Especially, it plays an important role in regulating the development of reproductive organs, and is an important regulatory factor for flower development and fruit maturation. In this paper, using Cucumis melo L.cv. Hetao, a melon variety, as the experimental material, all members of the MADS-box gene family were identified by bioinformatics method. A total of 62 MADS-box genes of muskmelon were identified, of which 26 belonged to type 鈪，

本文編號：1930083