發(fā)布時(shí)間：2018-05-09 21:09

  本文選題：豹貓 + 細(xì)胞培養(yǎng)�。� 參考：《內(nèi)蒙古大學(xué)》2017年碩士論文

【摘要】：對(duì)豹貓6種組織進(jìn)行原代培養(yǎng)建系,最后選擇3種組織來(lái)源細(xì)胞即劍狀軟骨源細(xì)胞、心臟源細(xì)胞和肺源細(xì)胞。以該3種細(xì)胞為實(shí)驗(yàn)材料研究豹貓細(xì)胞的生物學(xué)特性。在本研究中,主要進(jìn)行了如下實(shí)驗(yàn):細(xì)胞的原代建系及傳代培養(yǎng)的形態(tài)觀(guān)察、細(xì)胞貼壁率計(jì)算、凍存復(fù)蘇率對(duì)比、3種細(xì)胞生長(zhǎng)曲線(xiàn)的對(duì)比、支原體污染檢測(cè)、核型分析等。在形態(tài)學(xué)觀(guān)察方面,3種來(lái)源細(xì)胞各有顯著特征但均屬于成纖維樣細(xì)胞。貼壁率能力分析顯示,心臟源細(xì)胞最弱;肺源細(xì)胞代次P9之前易貼壁,P9之后細(xì)胞貼壁增殖及傳代比較困難;劍狀軟骨源細(xì)胞最易貼壁。將三種細(xì)胞凍存前后存活率進(jìn)行實(shí)驗(yàn)對(duì)比發(fā)現(xiàn):解凍后相比于凍存前均有所下降,隨著細(xì)胞代次的增高,細(xì)胞的存活率也有所下降。生長(zhǎng)曲線(xiàn)對(duì)比發(fā)現(xiàn),曲線(xiàn)均呈"S"型曲線(xiàn),曲線(xiàn)顯示劍狀軟骨源細(xì)胞增殖的最快,肺源細(xì)胞次之,心臟源細(xì)胞最慢。支原體污染檢測(cè)細(xì)胞污染情況為陰性。在豹貓細(xì)胞核型分析研究中發(fā)現(xiàn),染色體數(shù)目為2n=38,18對(duì)為常染色體,形態(tài)類(lèi)型為7M+1 1SM;1對(duì)為性染色體,其形態(tài)類(lèi)型X為ST。本研究為日后利用豹貓細(xì)胞、保存遺傳信息及深入研究其生物學(xué)特性提供了一個(gè)好的依據(jù)和實(shí)驗(yàn)材料。
[Abstract]:Six tissues of leopard cat were cultured and established in primary culture. Finally, three kinds of tissue derived cells, namely, swordlike chondrocytes, heart-derived cells and lung derived cells, were selected. The biological characteristics of leopard cat cells were studied by using these three kinds of cells as experimental materials. In this study, the following experiments were carried out: morphological observation of primary cell line and passage culture, calculation of cell adhesion rate, comparison of three cell growth curves, detection of mycoplasma contamination, karyotype analysis, etc. In the aspect of morphological observation, the three kinds of cells have distinct characteristics but all belong to fibroblast-like cells. The ability of adherent rate analysis showed that the heart derived cells were the weakest, the lung derived cells were easy to adhere to P9 before the passage of P9 and it was difficult to adhere to the proliferation and passage of the cells after P9, and the sword chondrocytes were the most likely to adhere to the wall. The survival rate of three kinds of cells before and after cryopreservation was compared with that before thawing, and the survival rate decreased with the increase of cell generation. The growth curves were found to be "S" curve, which showed the fastest proliferation of chondrocytes, followed by lung cells and heart cells. Mycoplasma contamination was negative for cell contamination. In the analysis of the karyotype of leopard cats, it was found that the number of chromosomes was 2nrn ~ (38), 18 pairs were autosomes, and 7M _ (11) SMN _ (1) pairs were sex chromosomes, and its morphological type X was ST. This study provides a good basis and experimental material for the future use of leopard cat cells, the preservation of genetic information and the further study of its biological characteristics.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：Q813.11

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