本文關(guān)鍵詞： 西藏血蜱 環(huán)境脅迫 抗寒物質(zhì) 抗氧化酶 丙二醛 西藏　出處：《河北師范大學》2017年碩士論文　論文類型：學位論文

【摘要】：西藏血蜱Haemaphysalis tibetensis Hoogstraal,1965僅分布于西藏和甘肅,是青藏高原地區(qū)重要的病原菌載體。目前已對西藏血蜱形態(tài)學、種群動態(tài)、生活史和分子系統(tǒng)學等方面做了研究,但有關(guān)其對環(huán)境的生理適應(yīng)研究尚屬空白。本研究通過測定環(huán)境變化后西藏血蜱雌蜱體內(nèi)水分和抗寒物質(zhì)含量的變化分析其對環(huán)境變化的生理響應(yīng);通過探討西藏血蜱體內(nèi)抗氧化酶活性和膜脂過氧化物含量的變化分析其對環(huán)境脅迫的適應(yīng)對策,為評價西藏血蜱的特殊地理分布和對環(huán)境的耐受性提供科學的依據(jù),主要結(jié)果如下:1.水分:與野外種群相比,實驗種群體內(nèi)自由水含量從55.65%上升至56.96%,雖有上升趨勢,但無顯著差異。2.抗寒物質(zhì):與野外種群相比,實驗種群體內(nèi)糖原、脂肪和可溶性蛋白含量均顯著升高(P0.05)。糖原含量從8.62 mg/g上升至11.16 mg/g;脂肪含量從16.39%上升至21.66%;可溶性蛋白含量從1.06 gprot/l上升至1.16 gprot/l。實驗種群血淋巴甘油含量顯著降低(P0.05),從12.21μg/ml下降至10.81μg/ml。3.抗氧化酶和丙二醛(MDA):隨西藏血蜱的發(fā)育,抗氧化酶活性和丙二醛含量均增加,雌蜱時期最高。與野外種群相比,實驗種群體內(nèi)保護酶活性和MDA含量均顯著增加(P0.05)。超氧化物歧化酶(SOD)活性從20.27 U/mgprot增至26.06 U/mgprot;過氧化氫酶(CAT)活性從16.9 U/mgprot增至20.06 U/mgprot;過氧化物酶(POD)活性從6.96 U/mgprot增至9.40 U/mgprot;MDA含量從12.35 nmol/mgprot增至14.48nmol/mgprot。谷胱甘肽硫轉(zhuǎn)移酶(GSTs)活性從87.67 U/mgprot增至90.66 U/mgprot,雖有上升趨勢,但差異不顯著。4.溫度對抗氧化酶和丙二醛的影響:以22°C西藏血蜱雌蜱體內(nèi)抗氧化酶活性和MDA含量作為對照,隨著溫度的降低SOD活性逐漸升高,除20℃外SOD活性與對照相比差異均顯著(P0.05);在-5℃和0℃時CAT活性顯著升高,在10℃時其活性顯著降低(P0.05);在-5℃、0℃和5℃時POD活性顯著升高(P0.05);在-5℃時GSTs顯著升高,在5℃、10℃和15℃時其活性顯著降低(P0.05);在5℃、10℃、15℃時MDA含量顯著降低(P0.05)。其余溫度條件下,抗氧化酶活性和MDA含量雖有變化,與對照相比差異不顯著。
[Abstract]:Haemaphysalis tibetensis Hoogstraalus is only distributed in Tibet and Gansu, and is an important pathogen carrier in Qinghai-Tibet Plateau. The morphology, population dynamics, life history and molecular phylogeny of Haemaphysalis Xizang have been studied. However, the study on physiological adaptation to the environment was still blank. The physiological response to environmental changes was analyzed by measuring the changes of water and cold-resistant substances in female tick of Haemaphysalis Tibet after environmental change. The changes of antioxidant enzyme activity and membrane lipid peroxide content in Haemaphysalis Xizang were studied to analyze their adaptation to environmental stress, which provided a scientific basis for evaluating the special geographical distribution and tolerance to the environment of Haemaphysalis Tibetan Haemaphysalis. The main results were as follows: water: compared with the wild population, the free water content in the experimental population increased from 55.65% to 56.96, although there was an upward trend, there was no significant difference between them. 2. Cold resistant substances: compared with the field population, the glycogen in the experimental population, The content of glycogen increased from 8.62 mg/g to 11.16 mg / g; the content of fat increased from 16.39% to 21.66; the content of soluble protein increased from 1.06 gprot/l to 1.16 g / L; the content of glycerol in hemolymph decreased significantly from 8.62 mg/g to 11.16 mg / g. 12.21 渭 g / ml decreased to 10.81 渭 g / ml. Antioxidant enzymes and malondialdehyde (MDA) MDAA: with the development of Haemaphysalis Tibetan, The activity of antioxidant enzymes and the content of malondialdehyde increased, and the female ticks were the highest in the period. The activity of protective enzyme and MDA increased significantly from 20.27 U / mgprot to 26.06 U / mg prot; catalase catalase activity increased from 16.9 U / mgprot to 20.06 U / mgprot; and the activity of peroxidase increased from 6.96 U / mgprot to 9.40 U / mgprot. From 12.35 nmol/mgprot to 14.48 nmol / mg prot.GST activity increased from 87.67 U / mgprot to 90.66 U / mg prott. But the difference was not significant. 4. The effect of temperature on oxidase and malondialdehyde: the activity of antioxidant enzyme and the content of MDA in female tick of Haemaphysalis Tibet increased with the decrease of temperature, and the activity of SOD increased gradually with the decrease of temperature. The activity of SOD was significantly increased at -5 鈩，

本文編號：1546184