本文關鍵詞：miRNA和高脂食物對達烏爾黃鼠糖脂代謝的影響　出處：《沈陽師范大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 達烏爾黃鼠 miRNA表達 糖脂代謝 高脂食物 能量代謝

【摘要】：為了探究miRNA及高脂食物對貯脂類冬眠動物達烏爾黃鼠(Spermophilus dauricus)從冬眠前育肥期至冬眠期這一過程中對能量選擇發(fā)生的調節(jié)機制及其適應性變化的影響,采用第二代轉錄組測序(RNA-Seq)技術,對達烏爾黃鼠起始育肥期、快速育肥期、育肥完成期和冬眠期四個階段的白色脂肪組織進行了測序,并分析了與代謝相關的miRNA差異表達情況。結果表明:在脂肪酸合成通路中,rno-miR-30a-3p、rno-miR-30e-3p在育肥完成期顯著上調,rno-miR-106b-3p在育肥完成期顯著上調。此時達烏爾黃鼠的體重呈下降趨勢,提示在此期間這些miRNA可能抑制脂肪酸合成;在糖酵解/糖異生通路中,rno-miR-139-5p、rno-mi R-185-5p、rno-miR-206-3p、rno-miR-23b-5p、rno-miR-29b-2-5p在快速育肥期和育肥完成期下調,rno-miR-30e-3p和rno-miR-135a-3p、rno-194-5p在快速育肥期下調,rno-miR-32-5p在完成育肥期下調,rno-miR-130b-3p、rno-miR-144-5p、rno-mi R-194-5p、rno-miR-196b-5p、rno-miR-449a-5p、rno-miR-451-5p在冬眠期上調;在TCA循環(huán)通路中,rno-miR-181a-5p、rno-miR-350、rno-mi R-7a-5p在冬眠期上調。從快速育肥期到完成育肥期,黃鼠體重快速增加到頂峰的過程,黃鼠的供能主要來自食物,以糖代謝為主。進入冬眠期,糖代謝降低,推測此時已轉化為脂代謝供能為主。為了探究高脂食物對達烏爾黃鼠糖脂代謝和冬眠的影響,通過對快速育肥期的達烏爾黃鼠喂以高脂食物,在飼喂前后對呼吸商和血糖穩(wěn)態(tài)進行測定,在飼喂期間對體重和能量收支進行測定,在飼喂之后的冬眠期進行冬眠陣的觀察。結果表明:1.在飼喂高脂食物的第60天高脂組體重顯著高于對照組,高脂組在第48天和第54天的消化率和可代謝能率高于對照組,推測高脂食物可能通過增加消化率和可代謝能效率增加體重,推遲完成育肥期的到來;2.高脂食物飼喂后的高脂組呼吸商顯著低于飼喂前,提示飼喂后高脂組代謝底物轉變?yōu)橐灾緸橹?3.高脂組血糖耐受性顯著低于對照組,胰島素敏感性沒有差異,推測高脂食物可能會導致;4.高脂食物飼喂一個月對冬眠情況可能并沒有影響。
[Abstract]:In order to explore the miRNA and high fat diet on lipid storage in the hibernating animal ground squirrel (Spermophilus dauricus) the choice of the regulating mechanism and adaptive changes influence on the energy from the front to the fattening period of hibernation hibernation this process, using the second generation transcriptome sequencing (RNA-Seq) technology of Citellus dauricus starting fast fattening period. The fattening period, the four stages of fattening period and complete hibernation of white adipose tissue was sequenced, and analyzed the difference of miRNA expression correlated with metabolism. The results showed that rno-miR-30a-3p in fatty acid synthesis pathway, and rno-miR-30e-3p was significantly up-regulated in fattening period, rno-miR-106b-3p period was significantly up-regulated in fattening. At this time of Citellus dauricus the body weight decreased, suggesting that during these miRNA may inhibit fatty acid synthesis; in glycolysis / gluconeogenesis pathway, rno-miR-139-5p, rno-mi, R-185-5p, R No-miR-206-3p, rno-miR-23b-5p, rno-miR-29b-2-5p in the fast fattening period and fattening period complete cut, rno-miR-30e-3p and rno-miR-135a-3p, rno-194-5p in the fast fattening period down, rno-miR-32-5p finished the fattening period down, rno-miR-130b-3p, rno-miR-144-5p, rno-mi, R-194-5p, rno-miR-196b-5p, rno-miR-449a-5p, rno-miR-451-5p in the hibernation period increases; in the TCA pathway, rno-miR-181a-5p, rno-miR-350, rno-mi and R-7a-5p in hibernation during the period of rise. From the fast fattening period to complete the fattening period, body weight increased rapidly to the peak of the ground squirrel, squirrel supply mainly from food to sugar metabolism. Into hibernation, reduced glucose metabolism, lipid metabolism has been transformed into speculation at this time can be based. In order to explore the effects of high fat diet on glucose and lipid metabolism in ground squirrels and through the rapid ur hibernation, fattening period of Citellus dauricus fed with high fat food, feed and The respiratory quotient and glucose homeostasis were measured in the feeding period on body weight and energy balance were measured after feeding in hibernation hibernation were observed. The results showed that 1. in sixtieth high fat group fed high fat food weight was significantly higher than the control group, high fat group in forty-eighth days and fifty-fourth days of digestion and the rate of metabolism was higher than the control group, high fat foods that could increase digestibility and metabolic efficiency can increase weight, delay the arrival of the fattening period; high fat group respiratory quotient 2. high fat diet after feeding was significantly lower than that before feeding, feeding after high fat group metabolic substrate changed to fatty. The high fat group 3.; glucose tolerance was significantly lower than the control group, there was no difference in insulin sensitivity, high fat food may lead to speculation; 4. fed a high-fat diet for one month may hibernate does not influence.

【學位授予單位】：沈陽師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q41

【參考文獻】

相關期刊論文 前6條

1 ZHONG Ling-Jun;XIE Zhi-Sheng;YANG Hua;LI Ping;XU Xiao-Jun;;Moutan Cortex and Paeoniae Radix Rubra reverse high-fat-diet-induced metabolic disorder and restore gut microbiota homeostasis[J];Chinese Journal of Natural Medicines;2017年03期

2 高文榮;朱萬龍;孟麗華;曹能;余婷婷;王政昆;;光周期和高脂食物對雌性高山姬鼠能量代謝和產(chǎn)熱的影響[J];生態(tài)學報;2013年18期

3 Samantha F.Kornfeld;Kyle K.Biggar;Kenneth B.Storey;;Differential Expression of Mature MicroRNAs Involved in Muscle Maintenance of Hibernating Little Brown Bats, Myotis lucifugus: A Model of Muscle Atrophy Resistance[J];Genomics, Proteomics & Bioinformatics;2012年05期

4 趙志軍;陳競峰;王德華;;光周期和高脂食物對布氏田鼠能量代謝和產(chǎn)熱的影響[J];動物學報;2008年04期

5 方曉紅，蔡益鵬;黃鼠下丘腦視前區(qū)NE能神經(jīng)支配對冬眠陣的調制[J];生理學報;1995年02期

6 黃欽恒,蔡益鵬,張崇理,王紅;達烏爾黃鼠冬眠前后腦內(nèi)單胺類神經(jīng)遞質及其代謝產(chǎn)物含量變化[J];生理科學;1988年05期

相關博士學位論文 前1條

1 朱萬龍;下丘腦神經(jīng)肽（NPY、AgRP、POMC、CART）在大絨鼠體重和能量代謝中調節(jié)作用的研究[D];南京師范大學;2015年

相關碩士學位論文 前2條

1 門麗媛;達烏爾黃鼠(Spermophilus dauricus)育肥過程和冬眠期白色脂肪組織中基因差異表達[D];沈陽師范大學;2016年

2 邢昕;達烏爾黃鼠（Spermophilus dauricus）冬眠以及育肥期間能量平衡調節(jié)機理的研究[D];沈陽師范大學;2011年

，

本文編號：1406592