本文關(guān)鍵詞：塞北荒漠羊柴AM真菌群落結(jié)構(gòu)和物種多樣性研究　出處：《河北大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： AM 真菌 PCR-DGGE 高通量測序 群落組成 羊柴 塞北荒漠

【摘要】：本論文以內(nèi)蒙古正藍(lán)旗青格勒?qǐng)D典型沙化梁地作為采樣地,分梁底、梁中和梁頂3個(gè)樣地采集羊柴(Hedysarum leave Maxim)根圍土壤樣品,通過Nested-PCR-DGGE和高通量測序技術(shù),結(jié)合DGGE圖譜分析、DNA克隆測序,研究AM真菌群落結(jié)構(gòu)和物種多樣性特征,為闡明沙化梁地AM真菌生態(tài)分布和功能提供依據(jù)。主要試驗(yàn)結(jié)果如下:1.DGGE研究結(jié)果表明,不同樣地和土層,AM真菌具有不同DGGE指紋圖譜特征,AM真菌豐度、優(yōu)勢度及其多樣性指數(shù)均有差異,其中梁中和梁頂樣地0-10 cm土層AM真菌豐度最高;多樣性指數(shù)梁中樣地0-10 cm土層最高。DGGE條帶全部序列歸為5屬15種,即Glomus 10種、Rhizophagus 1種、Funneliformis 2種、Paraglomus 1種、Septoglomus1種;Glomus和Funneliformis是沙化梁地AM真菌優(yōu)勢屬。聚類分析顯示AM真菌群落具有豐富的物種多樣性,相似性指數(shù)最高僅為0.54,說明梁地生境空間異質(zhì)性對(duì)AM真菌物種多樣性有顯著影響。2.Illumina HiSeq 2500高通量測序結(jié)果表明,通過以97%相似度進(jìn)行序列聚類,共得到486個(gè)OTUs,其中252個(gè)屬于Glomeromycota,全部序列歸于3綱,4目,10科和18個(gè)屬。其中Glomeromycota在梁底的豐度高于梁頂,Glomeraceae在所有樣品具有最高的相對(duì)豐富度。Sclerocytis和Glomus是所有樣本中豐度最高的屬,Clarodeoglomus,Rhizophagus和Gigaspora具有較高的相對(duì)豐度。Paraglomeraceae未在梁底發(fā)現(xiàn),Geosiphonaceae未在梁中發(fā)現(xiàn),Ambisporaceae和Pacisporaceae未在梁頂發(fā)現(xiàn)。3.冗余分析表明,土壤pH、有機(jī)碳(SOC)、全氮(TN)、總磷(TP)、有效氮(AVN)和有效磷(AVP)是影響AM真菌多樣性和物種組成的重要影響因素。不同坡位和土層能夠影響AM真菌群落組成和物種多樣性,使其AM真菌分布具有空間異質(zhì)性。
[Abstract]:This paper takes Inner Mongolia blue flag qinggelt typical Sandy Ridge as sampling points, the end of a beam, the collection of the Liang Zhonghe beam top 3 plots (Hedysarum leave Maxim) hedysarummaxim rhizosphere soil samples by Nested-PCR-DGGE and high-throughput sequencing technology, combined with the analysis of DGGE spectra, DNA cloning sequencing of AM fungal community structure and the characteristics of species diversity, provide the basis for clarifying the desertification ground AM fungi ecological distribution and function. The main results are as follows: the results of the 1.DGGE study, and equally soil fungi have AM fingerprint of different DGGE, AM fungi abundance, dominance and diversity index were different, in which the beam and beam top plot 0-10 cm AM the highest abundance of soil fungi; diversity index in 0-10 cm soil sample beam the highest.DGGE bands all sequences belonging to 5 genera and 15 species, namely Glomus 10, Rhizophagus 1, Funneliformis 2, Paraglomus 1 A kind of Septoglomus1, Glomus and Funneliformis are Sandy Ridge; AM fungi genera. Cluster analysis showed that the AM fungal community is rich in species diversity, the highest similarity index is only 0.54, that ridge habitat spatial heterogeneity has a significant impact on.2.Illumina HiSeq 2500 high-throughput sequencing results showed that the diversity of AM fungi species by sequence clustering with 97% similarity, there were 486 OTUs, 252 of which belong to Glomeromycota, all belong to sequence 3 classes, 4 orders, 10 families and 18 genera. Among them Glomeromycota in abundance is higher than that of top beam bottom beam, Glomeraceae has the highest relative abundance of.Sclerocytis and Glomus in all samples is abundance in all samples is the highest Clarodeoglomus, Rhizophagus and.Paraglomeraceae, the relative abundance of Gigaspora with high not found at the end of the beam, Geosiphonaceae was not found in the middle of the beam, Ambisporaceae and Pacis Poraceae is not found on the top of the beam.3. redundancy analysis showed that soil pH, organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), available nitrogen (AVN) and phosphorus (AVP) is an important factor influencing the composition of AM fungal diversity and species. Different slopes and soil fungi can influence AM community composition and species diversity, the distribution of AM fungi with spatial heterogeneity.

【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q938

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本文編號(hào)：1391726