本文關(guān)鍵詞：基于高通量數(shù)據(jù)的外源性植物miRNA跨界調(diào)控建模　出處：《吉林大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：micro RNA(mi RNA)是由21~23個核苷酸組成的一種非編碼小分子RNA。它在生物體內(nèi)通過降解靶m RNA或阻遏靶m RNA翻譯調(diào)控基因表達(dá),調(diào)控生物體細(xì)胞的生長、分化、凋亡等生物過程。已經(jīng)有研究證實,細(xì)菌、病毒的mi RNA在人體存在著跨界調(diào)控。近年來有研究發(fā)現(xiàn)植物mi RNA可以通過攝食進(jìn)入并穩(wěn)定存在于人體中,且保持其生物活性,使得外源性植物mi RNA是否對人體產(chǎn)生跨界調(diào)控成為mi RNA研究領(lǐng)域的熱點問題。為探究植物mi RNA在人體中可能存在的跨界調(diào)控作用,本文基于公開發(fā)表的數(shù)據(jù)對人體外源性植物mi RNA進(jìn)行挖掘,其中包括對已經(jīng)實驗證實的人體外源性植物mi RNA的數(shù)據(jù)以及目前人體血清的高通量mi RNA測序數(shù)據(jù)。對于高通量數(shù)據(jù),本文首先通過預(yù)處理過濾掉低質(zhì)量數(shù)據(jù),其次通過與植物mi RNA序列比對,獲得存在于人體的外源性植物mi RNA。最后在數(shù)據(jù)分析的基礎(chǔ)上,本文共得到54條存在于人體的外源性植物mi RNA,并以這些獲取的54條外源性植物mi RNA進(jìn)行植物-人體的跨界調(diào)控分析。所謂植物-人體的跨界調(diào)控分析,就是建立植物-人體跨界調(diào)控模型。首先對外源性植物mi RNA進(jìn)行外源性植物-人m RNA全基因組靶基因預(yù)測及篩選。其次,基于經(jīng)過兩次篩選得出的靶基因建立調(diào)控網(wǎng)絡(luò),并基于基因節(jié)點間存在的相互作用關(guān)系計算節(jié)點網(wǎng)絡(luò)權(quán)值,選取核心節(jié)點。最后,對核心節(jié)點進(jìn)行網(wǎng)絡(luò)核心模塊挖掘,分析核心模塊涉及的調(diào)控通路和參與的主要功能,即找到外源性植物mi RNA在人體中可能存在的調(diào)控作用。本文通過植物-人體跨界調(diào)控模型,挖掘出9個核心模塊,分別是AXIN1,HOXB1,ATP5D,MAPK8IP2,ZAP70,MTNR1B,GPR35和GYS1。結(jié)果表明,外源性植物mi RNA參與調(diào)控人體胺代謝、酒精代謝以及脂肪酸代謝等生物過程。由此可見,飲食習(xí)慣的不同,可能對人體的健康和生理機能產(chǎn)生影響。本文為通過高通量數(shù)據(jù)分析發(fā)現(xiàn)人體內(nèi)外源性植物mi RNA提供新方法,對于中國傳統(tǒng)中草藥mi RNA的治療模式具有理論的指導(dǎo)意義,可以從本質(zhì)上揭示植物的營養(yǎng)價值,對進(jìn)一步研究人類健康與日常飲食習(xí)慣的關(guān)系具有重要意義。
[Abstract]:Micro RNA (MI RNA) is a non coding small molecule RNA, which is composed of 21~23 nucleotides. It regulates the biological processes of cell growth, differentiation and apoptosis through the degradation of target m RNA or repressor target m RNA in vivo. It has been confirmed that the MI RNA of bacteria and virus has transboundary regulation in human body. In recent years, studies have found that plant mi RNA can enter and stably exist in human body through ingestion, and maintain its biological activity, making the exogenous plant mi RNA regulate the human body's transboundary regulation. It has become a hot topic in the research field of MI RNA. For cross-border regulation of plant mi RNA on may exist in the body, the mining of human exogenous plant mi RNA based on the published data, including the human body has been confirmed experimentally the exogenous plant mi RNA data and the current human serum high-throughput sequencing data of MI RNA. For high-throughput data, we first filtered low quality data through pretreatment, and then compared with plant mi RNA sequence, we obtained mi RNA which existed in human body. Finally, based on data analysis, 54 human mi RNA were obtained, and 54 transgenic plants, MI RNA, were used to analyze the transboundary regulation of plant human body. The so-called plant - human transboundary control analysis is to establish a plant - human transboundary regulation model. First, the whole genome target gene of exogenous plant - human M RNA was predicted and screened for exogenous plant mi RNA. Secondly, based on the target genes after two screening, we establish the regulatory network, and calculate the weight of the node network based on the interaction relationship between the gene nodes, and select the core nodes. Finally, the core nodes of the network were excavated, and the main functions of the core modules involved in the regulation were analyzed. That is to find the possible regulatory role of exogenous plants mi RNA in the human body. In this paper, through the plant - human transboundary control model, 9 core modules are excavated, which are AXIN1, HOXB1, ATP5D, MAPK8IP2, ZAP70, MTNR1B, GPR35 and GYS1. The results showed that the exogenous plant mi RNA was involved in the regulation of human amine metabolism, alcohol metabolism and fatty acid metabolism. It can be seen that different dietary habits may affect the health and physiological functions of the human body. This paper through the high-throughput data analysis found that the source of human body and plant mi RNA provide a new method and has theoretical significance for China traditional Chinese herbal medicine mi RNA treatment model can reveal the nutritional value of plants in nature, is of great significance for the further study on the relationship between human health and diet habits.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：Q78

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相關(guān)期刊論文 前1條

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本文編號：1343520