目前,微生物強(qiáng)化植物修復(fù)技術(shù)是修復(fù)受重金屬污染土壤的主要技術(shù)之一。本研究以受重金屬污染土壤中的微生物為研究對(duì)象,分析重金屬對(duì)土壤微生物群落多樣性和結(jié)構(gòu)的影響,同時(shí)分離生長(zhǎng)于鉛鋅礦區(qū)豆科植物（刺槐）根瘤中的內(nèi)生菌并篩選具有重金屬抗性及促植物生長(zhǎng)特性的根瘤菌,然后通過(guò)接種經(jīng)篩選的根瘤菌,以刺槐-根瘤菌共生體系來(lái)修復(fù)鉛鋅礦區(qū)重金屬污染土壤,研究該修復(fù)過(guò)程中根際土壤及根和根瘤內(nèi)微生物的群落結(jié)構(gòu)和多樣性變化,探討刺槐-根瘤菌共生體系對(duì)根際土壤微生物功能的影響。本研究的主要結(jié)論是:1.鉛鋅礦區(qū)冶煉廠的廢水影響土壤的微生物群落多樣性,改變其群落結(jié)構(gòu),這些變化具有空間差異性,而廢水中的重金屬是主要影響因素,且在重金屬重度污染的土壤中發(fā)現(xiàn)了更多的與硫循環(huán)和碳循環(huán)相關(guān)的微生物。本研究利用Illumina Mi Seq平臺(tái)基于16S rRNA基因V4-V5區(qū)測(cè)序技術(shù),分析了冶煉廠廢水處理沉淀池周邊不同位置的土壤微生物多樣性和群落結(jié)構(gòu)。研究發(fā)現(xiàn),微生物群落在四個(gè)采樣區(qū)域（入水口、上游、下游和出水口）具有顯著差異,但是在不同季節(jié)采集的樣品之間差異不顯著。限制性主坐標(biāo)分析表明,重金屬（Zn、Pb和Cu）、全硫、p... 

【文章來(lái)源】：西北農(nóng)林科技大學(xué)陜西省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：178 頁(yè)

【學(xué)位級(jí)別】：博士

【部分圖文】：

促植物生長(zhǎng)細(xì)菌對(duì)植物修復(fù)促進(jìn)機(jī)制的示意圖(Ullahetal.,2015)

go sativa 30 μM Cd Oxidative burst, necrosis, root hair damage Ortega-Villasantet al., 2005go sativa 30 μM Hg Oxidative burst, necrosis, root hair damage Ortega-Villasantet al., 2005e max-hizobiumcum 10950-500 μM Al Decreased number of nodules, activity ofN-assimilatory enzymes, and N2-fixation.Increased oxidative stressBalestrasse et 2006rietinum Pb (390 mg/kg soil) Number of nodules reduced by 13.7%,reduced plant growth, chlorophyll content,and N contentWani et al., 2007unguiculata-hizobiumB7562 μM Cu Complete inhibition of nodulation, shorteningand swelling at the root tips, number ofroot hairs decreasesKopittke et 2007rietinum Cd (23 mg/kg soil) Number of nodules reduced by 69%,reduced biomass, chlorophyll content, and N contentWani et al., 2007sativum-iumnosarum RP 5Cu (1,338 mg/kgsoil)Number of nodules reduced by 25%,N2-fixation was less affected, N contentin shoots reduced by 20%Wani et al., 2008go sativa-izobiume25 μM As Number of nodules decreases to 25%,number of rhizobial infections decreased by 90%,root hair damage, N2-fixation was not affectedPajuelo et al., 20

刺槐-根瘤菌共生體系在重金屬污染土壤修復(fù)過(guò)程中對(duì)土壤微生物的影響表 1-3 目前 NGS 技術(shù)的主要特點(diǎn)Table 1-3 Main characteristics of current NGS technologieschnologyRun type Maximum readlengthQualityscoresErrorSingle end Paired end Mate pairratesmina Yes Yes Yes 300 bp > 30 0.0034-1LiD Yes Yes Yes 75 bp > 30 0.01-1%Torrent Yes Yes No 400 bp ~ 20 1.78%4 Yes Yes No ~700 bp (up to 1 kb) > 20 1.07-1.7nopore Yes No No 5.4–10 kb NA 10-40%cBio Yes No No ~15 kb (up to 40 kb) < 10 5-10%

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