連續(xù)流亞硝化中試反應(yīng)器的啟動(dòng)及其能力提升
發(fā)布時(shí)間:2019-08-06 10:22
【摘要】:通過接種污水處理廠壓濾后污泥,添加懸浮填料進(jìn)行掛膜,采用連續(xù)流反應(yīng)器處理模擬氨氮污水,對(duì)反應(yīng)器的游離氨(FA)、游離亞硝酸(FNA)以及溶解氧(DO)進(jìn)行調(diào)控,實(shí)現(xiàn)了中試亞硝化反應(yīng)器的成功啟動(dòng).結(jié)果表明,通過前期高DO,后期低DO的運(yùn)行模式,并對(duì)反應(yīng)器運(yùn)行過程中的FA、FNA進(jìn)行調(diào)控實(shí)現(xiàn)了AOB的富集和NOB的淘汰,啟動(dòng)成功后反應(yīng)器內(nèi)部亞硝酸鹽產(chǎn)生速率(NPR)達(dá)到1.27 kg·(m~3·d)~(-1),亞硝酸鹽積累率(NAR)也穩(wěn)定在98%.采用實(shí)時(shí)熒光定量PCR方法(quantitative real time PCR,q PCR)對(duì)啟動(dòng)初期和成功啟動(dòng)后反應(yīng)器中的功能微生物(AOB、NOB)進(jìn)行分析,q PCR結(jié)果表明反應(yīng)功能微生物AOB的拷貝數(shù)從啟動(dòng)初期的5.3×10~9copies·m L~(-1)增長到1.6×10~(11)copies·m L~(-1),NOB的拷貝數(shù)反而從1.1×10~(10)copies·m L~(-1)下降到1.2×10~9copies·m L~(-1),AOB拷貝數(shù)的數(shù)量級(jí)比NOB的要高2個(gè)數(shù)量級(jí),這也是在啟動(dòng)過程中通過DO、FA、FNA等措施對(duì)NOB聯(lián)合抑制的作用.
[Abstract]:the suspended filler is added for hanging the membrane by inoculating the sludge after the pressure filtration of the sewage treatment plant, the simulated ammonia nitrogen sewage is treated by a continuous flow reactor, the free ammonia (FA), the free nitrous acid (FNA) and the dissolved oxygen (DO) of the reactor are regulated, And the successful starting of the pilot-scale nitrosation reactor is realized. The results showed that the concentration of the AOB and the phase-out of NOB were realized by the control and control of FA and FNA during the operation of the reactor, and the rate of nitrite production (NPR) in the reactor reached 1.27 kg 路 (m ~ 3 路 d) ~ (-1). The nitrite accumulation rate (NAR) was also stable at 98%. The functional microorganisms (AOB, NOB) in the reactor after the start-up and the successful start-up were analyzed by the real-time fluorescence quantitative PCR (q-PCR), The results of the q-PCR show that the number of copy number of the reaction-functional microorganism AOB is increased from 5.3 to 10 to 9 copies 路 m L-(-1) in the initial stage to 1.6 to 10-(11) copies 路 m L-(-1), and the copy number of the NOB is decreased from 1.1 to 10 to (10) copies 路 m L-(-1) to 1.2 to 10-9 copies 路 m L-(-1), and the order of magnitude of the AOB copy number is 2 orders of magnitude higher than that of the NOB, This is also the effect of the combination of DO, FA and FNA on the combination of NOB during the start-up.
【作者單位】: 蘇州科技大學(xué)環(huán)境科學(xué)與工程學(xué)院;蘇州科技大學(xué)環(huán)境生物技術(shù)研究所;
【基金】:國家自然科學(xué)基金項(xiàng)目(51478284,51408387) 國家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFC0401103) 江蘇省水處理技術(shù)與材料協(xié)同創(chuàng)新中心項(xiàng)目 江蘇省特色優(yōu)勢(shì)學(xué)科二期項(xiàng)目 蘇州市分離凈化材料與技術(shù)重點(diǎn)實(shí)驗(yàn)室項(xiàng)目(SZS201512)
【分類號(hào)】:X703
本文編號(hào):2523495
[Abstract]:the suspended filler is added for hanging the membrane by inoculating the sludge after the pressure filtration of the sewage treatment plant, the simulated ammonia nitrogen sewage is treated by a continuous flow reactor, the free ammonia (FA), the free nitrous acid (FNA) and the dissolved oxygen (DO) of the reactor are regulated, And the successful starting of the pilot-scale nitrosation reactor is realized. The results showed that the concentration of the AOB and the phase-out of NOB were realized by the control and control of FA and FNA during the operation of the reactor, and the rate of nitrite production (NPR) in the reactor reached 1.27 kg 路 (m ~ 3 路 d) ~ (-1). The nitrite accumulation rate (NAR) was also stable at 98%. The functional microorganisms (AOB, NOB) in the reactor after the start-up and the successful start-up were analyzed by the real-time fluorescence quantitative PCR (q-PCR), The results of the q-PCR show that the number of copy number of the reaction-functional microorganism AOB is increased from 5.3 to 10 to 9 copies 路 m L-(-1) in the initial stage to 1.6 to 10-(11) copies 路 m L-(-1), and the copy number of the NOB is decreased from 1.1 to 10 to (10) copies 路 m L-(-1) to 1.2 to 10-9 copies 路 m L-(-1), and the order of magnitude of the AOB copy number is 2 orders of magnitude higher than that of the NOB, This is also the effect of the combination of DO, FA and FNA on the combination of NOB during the start-up.
【作者單位】: 蘇州科技大學(xué)環(huán)境科學(xué)與工程學(xué)院;蘇州科技大學(xué)環(huán)境生物技術(shù)研究所;
【基金】:國家自然科學(xué)基金項(xiàng)目(51478284,51408387) 國家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFC0401103) 江蘇省水處理技術(shù)與材料協(xié)同創(chuàng)新中心項(xiàng)目 江蘇省特色優(yōu)勢(shì)學(xué)科二期項(xiàng)目 蘇州市分離凈化材料與技術(shù)重點(diǎn)實(shí)驗(yàn)室項(xiàng)目(SZS201512)
【分類號(hào)】:X703
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