發(fā)布時(shí)間：2018-11-18 14:57

【摘要】：本研究從汾河污泥中成功篩選出一株脫氮效果良好的異養(yǎng)硝化-好氧反硝化菌,將其命名為ZH-1。經(jīng)過(guò)生理生化鑒定和菌株16S rDNA序列分析,確定菌株ZH-1為施氏假單胞菌(Pseudomonas stutzeri),并建立了系統(tǒng)發(fā)育樹。菌株的異養(yǎng)硝化特性研究表明:菌株ZH-1可以將NH4~+-N直接轉(zhuǎn)化為氣態(tài)物質(zhì)而釋放,表現(xiàn)出良好的硝化作用。研究菌株ZH-1在不同培養(yǎng)條件下去除NH4~+-N的能力,實(shí)驗(yàn)得出菌株ZH-1在pH為8.0,接種量為2%,搖床轉(zhuǎn)速為100rpm,C/N為12,NH4~+-N濃度為100.0 mg/L,培養(yǎng)溫度為30°C時(shí)對(duì)NH4~+-N的降解效果最好,去除率達(dá)到99%。在好氧條件下,當(dāng)初始硝酸鉀濃度約為3g/L時(shí),菌株ZH-1表現(xiàn)出很好的反硝化作用,去除率可以達(dá)到100%,且在培養(yǎng)結(jié)束沒(méi)有檢測(cè)到亞硝酸鹽的存在。另外,研究菌株ZH-1在不同條件下去除NO_3~--N的能力,發(fā)現(xiàn)菌株ZH-1在pH為8.0,接種量為4%,轉(zhuǎn)速為100rpm,C/N為8,培養(yǎng)溫度為30°C,可以將約為419.0mg/L的NO_3~--N降解至1.4mg/L,去除率也可達(dá)到99.67%。利用菌株ZH-1對(duì)實(shí)際污水脫氮效果進(jìn)行了初步的實(shí)驗(yàn)。發(fā)現(xiàn)在未滅菌的污水中不添加小分子碳源和添加小分子碳源的狀態(tài)下,菌株ZH-1對(duì)實(shí)際污水的脫氮能力分別為34.95%和68.51%,這也說(shuō)明菌株對(duì)實(shí)際污水脫氮效果受到可利用碳源的影響;在滅菌污水中不添加小分子碳源和添加小分子碳源的狀態(tài)下,菌株ZH-1對(duì)污水的脫氮能力分別為14.73%和65.5%。通過(guò)比較也說(shuō)明了所取得實(shí)驗(yàn)污水里本身存在的一些菌也具有一定的脫氮能力,添加菌株ZH-1后可以促進(jìn)污水中氨氮含量的降低。所有的試驗(yàn)表明,分離得到的菌株ZH-1表現(xiàn)出較強(qiáng)的同時(shí)硝化反硝化的能力,在未來(lái)含氮廢水處理方面成為一株可利用的候選菌株,將來(lái)具有廣闊的應(yīng)用前景。
[Abstract]:In this study, a heterotrophic nitrification-aerobic denitrifying strain was successfully screened from the sludge of Fenhe River and named ZH-1.. After physiological and biochemical identification and 16s rDNA sequence analysis, the strain ZH-1 was identified as Pseudomonas sp. (Pseudomonas stutzeri), and phylogenetic tree was established. The study of heterotrophic nitrification of the strain showed that strain ZH-1 could directly transform NH4~ N into gaseous substance and release it, showing a good nitrification effect. The ability of strain ZH-1 to remove NH4~ N under different culture conditions was studied. The results showed that the strain ZH-1 was 8. 0 at pH, inoculation amount was 2 and rotation speed was 100rpmcn = 12nNH4 ~ -N, and the concentration of strain ZH-1 was 100.0 mg/L,. When the culture temperature was 30 擄C, the degradation efficiency of NH4~-N was the best, and the removal rate reached 99%. Under aerobic conditions, when the initial concentration of potassium nitrate was about 3g/L, strain ZH-1 showed a good denitrification effect, the removal rate could reach 100%, and no nitrite was detected at the end of culture. In addition, the ability of the strain ZH-1 to remove NO_3~--N under different conditions was studied. It was found that the ZH-1 of the strain was 8.0 at pH, the inoculation amount was 4, the rotational speed was 100 rpm ~ (-1) C / N = 8, and the culture temperature was 30 擄C. The NO_3~--N about 419.0mg/L can be degraded to 1.4 mg / L, and the removal rate can reach 99.67%. A preliminary experiment was carried out on the nitrogen removal effect of actual wastewater by using strain ZH-1. It was found that the denitrification ability of the strain ZH-1 to actual sewage was 34.95% and 68.51%, respectively, without adding small molecular carbon source and adding small molecular carbon source in unsterilized sewage. This also shows that the nitrogen removal effect of the strain on the actual sewage is affected by the available carbon source. The denitrification capacity of the strain ZH-1 was 14.73% and 65.5%, respectively, when there were no small molecular carbon sources or small molecular carbon sources added in the sterilized sewage. The comparison also showed that some bacteria contained in the sewage also had certain denitrification ability, and the addition of strain ZH-1 could promote the decrease of ammonia nitrogen content in the sewage. All the experiments showed that the isolated strain ZH-1 showed strong ability of simultaneous nitrification and denitrification, and was a candidate strain for the treatment of nitrogen-containing wastewater in the future, and had a broad application prospect in the future.
【學(xué)位授予單位】：山西師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：X172;Q93

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