【摘要】：腐殖生態(tài)基對(duì)強(qiáng)化活性污泥工藝脫氮除磷和改善活性污泥性質(zhì)方面具有較好的作用。本文在考察腐殖生態(tài)基對(duì)SBR工藝脫氮除磷效能影響的基礎(chǔ)上,通過(guò)設(shè)置不同水溫、不同運(yùn)行方式來(lái)研究水溫和運(yùn)行方式對(duì)腐殖生態(tài)基SBR工藝污染物去除效果的影響,并利用高通量測(cè)序方法研究微生物群落結(jié)構(gòu)的變化,為該工藝的進(jìn)一步發(fā)展與應(yīng)用提供一定的理論基礎(chǔ)。外置SBR和傳統(tǒng)SBR在25℃水溫條件下對(duì)比試驗(yàn)結(jié)果表明,腐殖生態(tài)基對(duì)SBR工藝COD的去除影響不大,但對(duì)NH4+-N和TP的去除率分別提高了5.9%和7.4%。水溫分別控制在10℃、15℃、25℃的外置SBR對(duì)比試驗(yàn)結(jié)果表明,水溫對(duì)COD的去除情況影響不大,水溫升高有利于NH4+-N的去除,但不利于TP的去除。高通量測(cè)序分析結(jié)果表明,溫度的升高有利于微生物菌群豐度和多樣性的提高。三反應(yīng)器活性污泥樣品中Rhodocyclaceae的相對(duì)豐度分別為20.62%、15.22%、11.34%,Aeromonas的相對(duì)豐度分別為2.12%、0.81%、0.1%,均隨水溫的升高呈遞減趨勢(shì),而Comamonadaceae的相對(duì)豐度分別為3.65%、4.16%、10.42%,隨水溫的升高呈遞增趨勢(shì),這與不同水溫下NH4+-N和TP去除效果的變化相一致。外置SBR和內(nèi)置SBR在25℃水溫條件下的對(duì)比試驗(yàn)結(jié)果表明,腐殖生態(tài)基外置和內(nèi)置對(duì)COD的去除效果差別不大,而對(duì)NH4+-N、TP的去除,內(nèi)置反應(yīng)器分別高于外置反應(yīng)器2.2%、3.8%,可見(jiàn)腐殖生態(tài)基內(nèi)置有利于氨氮和總磷的去除。高通量測(cè)序分析結(jié)果表明,內(nèi)置反應(yīng)器的微生物豐富要優(yōu)于外置反應(yīng)器,而多樣性無(wú)明顯區(qū)別。內(nèi)置反應(yīng)器活性污泥樣品中Rhodocyclaceae、Xanthomonadaceae的相對(duì)豐度分別高于外置樣品4.27%、1.9%,這與腐殖生態(tài)基內(nèi)置有助于除磷相一致。內(nèi)置樣品中Comamonadaceae、Thauera的相對(duì)豐度分別高于外置樣品2.82%、4.26%,這與腐殖生態(tài)基內(nèi)置有助于氨氮的去除相一致。好氧/厭氧與厭氧/好氧兩種運(yùn)行方式的內(nèi)置SBR系統(tǒng)在10℃水溫條件下對(duì)比試驗(yàn)結(jié)果表明,好氧與厭氧的順序,對(duì)系統(tǒng)COD、TP的去除率影響不大,但由典型周期變化規(guī)律可知好氧/厭氧SBR系統(tǒng)COD、TP的降解速率要快于厭氧/好氧運(yùn)行方式,而對(duì)于NH4+-N的去除,好氧/厭氧要高于厭氧/好氧4.3%左右,有利于氨氮的去除。高通量測(cè)序分析結(jié)果表明,好氧/厭氧反應(yīng)器的微生物菌群豐度和多樣性都大于厭氧/好氧反應(yīng)器。Aeromonas較原泥分別增長(zhǎng)了5.02%、1.79%,Thauera較原泥分別增長(zhǎng)了3.49%、2.71%,Comamonadaceae較原泥分別升高了4.74%、2.72%。這與好氧/厭氧條件下NH4+-N和TP較好的去除效果相一致。
[Abstract]:Humic ecological base plays a better role in denitrification and phosphorus removal of activated sludge process and improving the properties of activated sludge. On the basis of investigating the effect of humic ecological base on nitrogen and phosphorus removal efficiency of SBR process, the effects of water temperature and operation mode on the removal of pollutants from humic ecological SBR process were studied by setting different water temperature and different operation mode. The changes of microbial community structure were studied by high-throughput sequencing method, which provided a theoretical basis for the further development and application of this process. The results showed that humic ecological base had little effect on COD removal of SBR process, but the removal rates of NH4 N and TP were increased by 5.9% and 7.4% respectively. The results of external SBR experiments with water temperature controlled at 10 鈩，

本文編號(hào)：2168625