【摘要】：為研究有機污染物對海洋生物的毒性影響,選擇海洋環(huán)境中典型的異戊二烯作為暴露污染物,研究其對體質(zhì)量為(5.23±0.96)g刺參Apostichopus japonicus的急性毒性及其體腔液中超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、酸性磷酸酶(ACP)和堿性磷酸酶(AKP)活性的影響,并在實驗室條件下,采用靜水式生物毒性試驗法開展了急性毒性試驗,以及96 h亞致死效應(yīng)的毒性試驗。結(jié)果表明:異戊二烯對刺參的24、48、72、96 h半致死質(zhì)量濃度分別為83.00、78.09、72.93、69.77 mg/L,安全質(zhì)量濃度為6.98 mg/L;亞致死試驗中,CAT活性受到的影響最大,表現(xiàn)為受到抑制作用,存在良好的劑量-效應(yīng)和時間-效應(yīng)正相關(guān)性;96 h時,中、低濃度異戊二烯對SOD活性具有顯著的誘導(dǎo)作用(P0.05),而高濃度異戊二烯對SOD活性具有極顯著的抑制作用(P0.01);ACP和AKP活性受到的影響較小,但48 h時兩種酶均受到極顯著的誘導(dǎo)作用(P0.01),96 h時AKP活性受到顯著的誘導(dǎo)作用(P0.05);隨著異戊二烯質(zhì)量濃度的增加和時間的延長,刺參體腔液中4種酶活性受到不同程度的影響,因此,異戊二烯脅迫對刺參體內(nèi)產(chǎn)生自由基導(dǎo)致氧化脅迫效應(yīng),以及對消化、能量代謝系統(tǒng)的影響可能是其重要的致毒原因,刺參體腔液中4種酶的變化體現(xiàn)了異戊二烯的致毒機制。本研究結(jié)果可為異戊二烯對海洋生物的生態(tài)毒理學(xué)方面的研究提供參考依據(jù)。
[Abstract]:In order to study the toxic effects of organic pollutants on marine organisms, the typical isoprene in the marine environment was selected as exposure pollutants. The acute toxicity of Apostichopus japonicus and the activities of superoxide dismutase (SOD),) catalase (CAT),) acid phosphatase (ACP) and alkaline phosphatase (AKP) in the body cavity of Acanthopsis japonicus were studied. The acute toxicity test and the 96 h sublethal toxicity test were carried out by hydrostatic biotoxicity test. The results showed that the semi-lethal concentration of isoprene to sea cucumber was 83.00 (78.09) (72.93) 渭 g / L and the safe concentration was 6.98 mg / L, respectively, and the activity of catalase was the most affected in the sublethal test, which showed that the activity of catalase was inhibited, and the concentration of isoprene was 69.77 mg / L and 6.98 mg / L, respectively, in the sublethal test. There was a good dose-effect and time-effect positive correlation at 96 h. Low concentration isoprene could induce SOD activity significantly (P0.05), while high concentration isoprene had extremely significant inhibitory effect on SOD activity (P0.01). However, at 48 h, both of the two enzymes were significantly induced (P0.01) and the activity of AKP was significantly induced at 96 h (P0.05), and with the increase of isoprene concentration and the prolongation of time, the activities of four enzymes in the body cavity fluid of Acanthopsis japonicus were affected to some extent. Therefore, the effects of isoprene stress on the production of free radicals in the body of Acanthopsis japonicus and the effects of isoprene stress on digestion and energy metabolism may be important causes of its toxicity. The changes of four enzymes in the body cavity of Acanthopsis japonicus showed the toxic mechanism of isoprene. The results of this study may provide a reference for the study of ecotoxicology of isoprene to marine organisms.
【作者單位】： 遼寧省海洋水產(chǎn)科學(xué)研究院遼寧省海洋生物資源和生態(tài)學(xué)重點實驗室;大連市水產(chǎn)研究所;
【基金】：遼寧省海洋與漁業(yè)廳科研項目(201417)
【分類號】：X171.5

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