本文選題：氧氟沙星 + 重金屬銅��； 參考：《昆明理工大學(xué)》2017年碩士論文

【摘要】：抗生素的濫用使其通過多種途徑進(jìn)入環(huán)境,從而產(chǎn)生健康風(fēng)險(xiǎn)。重金屬在土壤及水體環(huán)境中普遍存在,其隱蔽性強(qiáng)的特性導(dǎo)致它容易通過食物鏈富集,造成動植物出現(xiàn)重金屬中毒�？股丶爸亟饘僭诃h(huán)境中的共存現(xiàn)象非常普遍,并且抗生素易與重金屬離子發(fā)生絡(luò)合反應(yīng),使它們的環(huán)境風(fēng)險(xiǎn)評估變得更加復(fù)雜。研究抗生素與重金屬對環(huán)境中生物的復(fù)合風(fēng)險(xiǎn)勢在必行。本研究選擇抗生素中的代表性物質(zhì)氧氟沙星(OFL)和重金屬中的銅離子(Cu~(2+))作為實(shí)驗(yàn)對象,對離體細(xì)胞培養(yǎng)中的模式細(xì)胞非洲綠猴腎細(xì)胞(Vero)進(jìn)行復(fù)合污染物毒性實(shí)驗(yàn),以此研究抗生素OFL與Cu~(2+)及其復(fù)合污染物對Vero細(xì)胞的生物效應(yīng)。通過對比OFL、Cu~(2+)及其復(fù)合污染物對Vero細(xì)胞的毒性效應(yīng)的差異研究復(fù)合污染與單獨(dú)污染的區(qū)別,并進(jìn)一步探討影響氧氟沙星和Cu~(2+)絡(luò)合物形成的影響因素(pH值和溫度)及復(fù)合絡(luò)合體系對Vero細(xì)胞的毒性效應(yīng)。所以本研究對抗生素與重金屬復(fù)合污染的環(huán)境風(fēng)險(xiǎn)研究具有一定理論和實(shí)踐價(jià)值。結(jié)果表明:(1)OFL與Cu~(2+)及其復(fù)合污染物會導(dǎo)致細(xì)胞形態(tài)變化,細(xì)胞破碎,貼壁率降低。當(dāng)Cu~(2+)濃度從6.88 mg·L-1增加到6875 mg·L-1時(shí),其對Vero細(xì)胞生長的抑制率也上升了 55%。實(shí)驗(yàn)表明,OFL對Vero細(xì)胞的生長也有顯著的影響,當(dāng)OFL濃度低于3mg·L-1時(shí),OFL促進(jìn)了 Vero細(xì)胞的生長;而當(dāng)OFL濃度高于3 mg·L-1時(shí),Vero細(xì)胞的生長則被抑制。(2)氧氟沙星和銅復(fù)合污染對Vero細(xì)胞的毒性與其污染物單獨(dú)污染時(shí)的毒性之和相比有所下降。在2.75 mg·L-1Cu~(2+)濃度時(shí),OFL濃度從0.63 mg·L-1增加到10 mg· L-1時(shí)其復(fù)合污染物對細(xì)胞的抑制率從24%上升到了最高47%。當(dāng)OFL濃度為 2.5 mg·L-1、5 mg·L-1 和 10 mg·L-1 時(shí),OFL 與濃度為 2.75 mg·L-1 Cu~(2+)的復(fù)合物對細(xì)胞的抑制率與OFL、Cu~(2+)單一抑制率之和有顯著差異,呈現(xiàn)出抑制率下降的趨勢。同時(shí),當(dāng)Cu~(2+)濃度6.88mg·L-1處理下時(shí)出現(xiàn)了類似的現(xiàn)象,OFL與濃度為6.88 mg· L-1 Cu~(2+)的復(fù)合物對細(xì)胞抑制率顯著低于OFL、Cu~(2+)單一抑制率之和。(3)DMEM培養(yǎng)基中OFL與Cu~(2+)的游離的量要顯著低于水環(huán)境中兩者游離的量。說明OFL和Cu~(2+)會與DMEM培養(yǎng)基中其他物質(zhì)發(fā)生反應(yīng),形成一個(gè)由OFL與Cu~(2+)為主的復(fù)合絡(luò)合體系。(4)溫度和pH值的變化能夠?qū)MEM細(xì)胞培養(yǎng)液中的OFL-Cu~(2+)復(fù)合絡(luò)合體系總濃度產(chǎn)生顯著的影響。隨著溫度的升高,OFL-Cu~(2+)復(fù)合絡(luò)合體系總濃度逐漸增大,這說明溫度的升高不但增大了氧氟沙星和銅離子之間的相互作用,還提高了它們與DMEM培養(yǎng)液中其他物質(zhì)的反應(yīng)活性。同時(shí),pH值的增大也使得OFL-Cu~(2+)復(fù)合絡(luò)合體系的總濃度上升,這說明pH值的增大能夠提高氧氟沙星與銅的反應(yīng)活性及其絡(luò)合能力。(5)OFL-Cu~(2+)復(fù)合絡(luò)合體系不會對Vero細(xì)胞的生長產(chǎn)生毒性效應(yīng)。OFL和Cu~(2+)復(fù)合污染毒性與單獨(dú)污染毒性之和相比有所下降的主要原因之一是在氧氟沙星與銅復(fù)合污染體系中DMEM細(xì)胞培養(yǎng)液、OFL和Cu~(2+)相互之間發(fā)生了反應(yīng),形成了 OFL-Cu~(2+)復(fù)合絡(luò)合體系,而該體系對Vero細(xì)胞的生長產(chǎn)生的影響不顯著,從而降低了氧氟沙星和銅復(fù)合污染的總體毒性。由此看來,抗生素OFL與重金屬Cu~(2+)對Vero細(xì)胞的復(fù)合毒性會因?yàn)镺FL-Cu~(2+)復(fù)合絡(luò)合體系的產(chǎn)生而下降。說明OFL與Cu~(2+)對Vero細(xì)胞生長的毒性效應(yīng)具有拮抗作用。表明環(huán)境中污染物間可能存在拮抗作用使復(fù)合污染對環(huán)境的風(fēng)險(xiǎn)下降。
[Abstract]:The abuse of antibiotics causes it to enter the environment through a variety of ways, resulting in health risks. Heavy metals are prevalent in the soil and water environment. Their concealed and strong characteristics cause it to be easily enriched through the food chain, causing heavy metal poisoning in animals and plants. The coexistence of antibiotics and heavy metals in the environment is very common and resistant to the environment. In this study, the representative substances ofofloxacin (OFL) and copper ions (Cu~ (2+)) in heavy metals (Cu~ (2+)) are selected as the experimental object, and in vitro finer. The cytotoxic effects of antibiotic OFL and Cu~ (2+) and their compound pollutants on Vero cells were studied in the cytotoxicity test of cell culture model cells, African green monkey kidney cells (Vero), and the difference between compound pollution and single pollution was studied by comparing the differences of OFL, Cu~ (2+) and its compound pollutants to Vero cells. The influence factors of the formation of ofloxacin and Cu~ (2+) complex formation (pH value and temperature) and the toxic effect of complex complex system on Vero cells are further explored. Therefore, this study has a certain theoretical and practical value on the environmental risk study of the compound pollution of antibiotics and heavy metals. The results show that: (1) OFL and Cu~ (2+) and its compound pollutants will be found. When Cu~ (2+) concentration increased from 6.88 mg. L-1 to 6875 mg. L-1, the inhibition rate to the growth of Vero cells was also increased by 55%. experiment, which showed that OFL had a significant influence on the growth of Vero cells, and when OFL concentration was lower than 3mg. The growth of Vero cells was inhibited when higher than 3 mg. L-1. (2) the toxicity of the compound pollution of ofloxacin and copper to Vero cells was decreased compared with the toxicity of the contaminants alone. At the concentration of 2.75 mg L-1Cu~ (2+), the concentration of OFL from 0.63 mg L-1 increased to 10 mg L-1. The inhibitory rate of the compound pollutants to the cells was 24%. When the concentration of OFL was 2.5 mg. L-1,5 mg, L-1 and 10 mg. L-1, the inhibition rate of OFL and concentration of 2.75 mg. L-1 Cu~ was significantly different from that of the single inhibitory rate. The cell inhibition rate of OFL and the concentration of 6.88 mg. L-1 Cu~ (2+) was significantly lower than that of OFL, Cu~ (2+) single inhibition rate. (3) the free amount of OFL and Cu~ (2+) in the DMEM medium was significantly lower than that in the water environment. 2+) complex complex system. (4) the change of temperature and pH value can significantly influence the total concentration of OFL-Cu~ (2+) complex complex system in DMEM cell culture. With the increase of temperature, the total concentration of OFL-Cu~ (2+) complex complex system increases gradually. This shows that the increase of temperature not only increases the phase between ofloxacin and copper ions. The interaction between them and other substances in the DMEM culture also increased their reactivity to other substances in the DMEM culture. At the same time, the increase of the pH value also increased the total concentration of the OFL-Cu~ (2+) complex complex system, which indicated that the increase of the pH value could improve the reactivity and complexation ability of ofloxacin and copper. (5) the OFL-Cu~ (2+) complex complex system would not produce Vero cells. One of the main reasons for the decrease of the toxic effect of.OFL and Cu~ (2+) on the toxicity of the compound pollution toxicity of the DMEM cell in the compound pollution system of ofloxacin and copper is the reaction between OFL and Cu~ (2+) in the compound pollution system of ofloxacin and copper, and the complex complex system of OFL-Cu~ (2+) is formed, and the growth of Vero cells in this system The effect of the production is not significant, thus reducing the overall toxicity of the compound pollution of ofloxacin and copper. Therefore, the compound toxicity of antibiotic OFL and heavy metal Cu~ (2+) to Vero cells will be reduced by the production of OFL-Cu~ (2+) complex complex system. It shows that the toxic effects of OFL and Cu~ (2+) on the growth of Vero cells are antagonistic. There may be antagonism among pollutants, which makes the risk of compound pollution to environment decrease.
【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：X171.5

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