本文選題：焦化廢水 + 市政污泥��； 參考：《環(huán)境科學(xué)》2017年09期

【摘要】：焦化廢水是毒性很大的典型工業(yè)廢水,生物處理過程中需要微生物具備很強(qiáng)的適應(yīng)能力.以探討焦化廢水對(duì)微生物的毒性抑制以及微生物對(duì)焦化廢水的適應(yīng)過程為目的,通過于焦化廢水原水中接種市政污泥,在考察COD、苯酚、氨氮和硫氰化物等主要污染物指標(biāo)降解的基礎(chǔ)上,運(yùn)用Illumina高通量測(cè)序平臺(tái)分析降解過程微生物群落組成及多樣性變化的響應(yīng)關(guān)系.結(jié)果表明,接種了市政污泥的焦化廢水培養(yǎng)16 h后COD開始下降,40 h時(shí)苯酚降解了97.14%,72 h時(shí)硫氰化物開始降解,96 h時(shí)硫氰化物濃度低于檢測(cè)限,氨氮濃度隨著硫氰化物的降解而升高.群落測(cè)序分析表明,不同培養(yǎng)階段污泥中微生物表現(xiàn)出群落結(jié)構(gòu)及豐度上的差異:在苯酚降解階段,苯酚優(yōu)勢(shì)降解菌Acinetobacter、Pseudomonas的豐度增大,48 h總相對(duì)豐度為13.04%;在硫氰化物降解階段,Sphingobacterium、Brevundimonas、Lysobacter、Chryseobacterium為主導(dǎo)菌屬,96 h時(shí)其總相對(duì)豐度為16.13%;在144 h階段,優(yōu)勢(shì)菌屬則變?yōu)镕luviicola、Stenotrophomonas和Thiobacillus,總相對(duì)豐度為22.45%.由此認(rèn)為,市政污泥克服了焦化廢水中毒性成分的抑制作用之后能迅速適應(yīng)環(huán)境,表現(xiàn)出微生物群落結(jié)構(gòu)隨著廢水降解成分的變化而改變,環(huán)境因子和降解功能菌之間的競爭是群落結(jié)構(gòu)演變的主要因素.
[Abstract]:Coking plant wastewater is a typical industrial wastewater with high toxicity. It is necessary for microorganisms to have strong adaptability in the process of biological treatment. In order to study the inhibition of microbial toxicity of coking wastewater and the adaptation of microorganisms to coking wastewater, municipal sludge was inoculated in raw wastewater of coking plant, and COD and phenol were investigated. Based on the degradation of main pollutants such as ammonia nitrogen and thiocyanide, the response relationship of microbial community composition and diversity changes during degradation was analyzed by Illumina high-throughput sequencing platform. The results showed that the COD of coking wastewater inoculated with municipal sludge decreased at 40 h after incubation, and the degradation of phenol was 97.14 h and the concentration of thiocyanate was lower than the detection limit at 96 h when thiocyanate was degraded at 72 h. The concentration of ammonia nitrogen increased with the degradation of thiocyanate. Community sequencing analysis showed that the microbial community structure and abundance were different in different culture stages: in phenol degradation stage, The total relative abundance of acinetobacter Pseudomonas in 48 h was 13.04, the total relative abundance of Sphingobacter Brevundimonia Lysobacter Chryseobacterium was 16.1313 at 96 h, and at 144 h, the dominant genus became Fluviicolaena Stenotrophomonas and Thiobacillus. the total relative abundance was 22.45. It is concluded that municipal sludge can quickly adapt to the environment after overcoming the inhibition of toxic components in coking wastewater, and show that the microbial community structure changes with the change of degradation composition of wastewater. Competition between environmental factors and biodegradable functional bacteria is the main factor in the evolution of community structure.
【作者單位】： 華南理工大學(xué)生物科學(xué)與工程學(xué)院;廣州市市政工程維修處;華南理工大學(xué)環(huán)境與能源學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(21377040) 廣東省省級(jí)科技計(jì)劃項(xiàng)目(2015A020215008) 廣東省應(yīng)用型科技研發(fā)專項(xiàng)(2015B020235005)
【分類號(hào)】：X172;X784

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