發(fā)布時(shí)間：2018-05-31 01:42

  本文選題：農(nóng)藥 + 四元混合物��； 參考：《安徽農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年05期

【摘要】：以發(fā)光菌青海弧菌(Vibrio qinghaiense sp.-Q67)為指示生物,4種農(nóng)藥包括殘殺威、甲霜靈、多果定和西草凈為研究對(duì)象,應(yīng)用均勻試驗(yàn)設(shè)計(jì)法設(shè)計(jì)農(nóng)藥的四元混合物體系,共7條射線,應(yīng)用微板毒性測(cè)試方法(MTA)系統(tǒng)測(cè)定4種農(nóng)藥及其四元混合物體系對(duì)Q67的發(fā)光抑制毒性,采用非線性最小二乘法擬合濃度-效應(yīng)數(shù)據(jù),以濃度加和模型(CA)為標(biāo)準(zhǔn)加和參考模型分析混合物毒性相互作用。結(jié)果表明,Logit或Weibull函數(shù)可有效地?cái)M合4種農(nóng)藥的濃度 效應(yīng)數(shù)據(jù),其相關(guān)系數(shù)R0.99,均方根誤差RMSE0.022;4種農(nóng)藥對(duì)發(fā)光菌Q67的毒性大小順序?yàn)槎喙?p EC50=5.35)西草凈(p EC50=3.56)甲霜靈(p EC50=3.18)殘殺威(p EC50=3.00),其中多果定毒性最大,殘殺威、甲霜靈和西草凈對(duì)Q67的毒性比較接近;四元農(nóng)藥混合物體系的7條射線對(duì)Q67的毒性(p EC50值)與組分西草凈和多果定的濃度比尤其是西草凈和多果定的濃度比之和具有良好的正相關(guān)關(guān)系(R=0.8729,RMSE=0.076),但與殘殺威和甲霜靈的濃度比之和具有良好的負(fù)相關(guān)關(guān)系(R=0.8729,RMSE=0.076);CA模型能很好地評(píng)估四種農(nóng)藥的四元混合物體系對(duì)Q67的毒性,即混合物體系呈經(jīng)典的加和作用。
[Abstract]:Vibrio qinghaiense sp.-Q67) was used as indicator for four kinds of pesticides, including propoxur, Metalaxyl, Duoguodin and Xicaojing, and the four-component mixture system of pesticides was designed by uniform experimental design method, which was composed of 7 rays, and was used for the study of the chemical composition of Vibrio qinghaiense sp.-Q67 (Vibrio qinghaiense sp.-Q67). The luminescence inhibition toxicity of four pesticides and their quaternary mixtures to Q67 was determined by using the microplate toxicity test method (MTAA). The concentration effect data were fitted by nonlinear least square method. The toxicity interaction of mixtures was analyzed by using the concentration sum model (CAA) as the standard additive model and the reference model. The results showed that the logit or Weibull function could fit the concentration effect data of four pesticides effectively. The correlation coefficient R0.99, root mean square error (RMSE 0.022), the toxicity order of four pesticides to the luminescent bacteria Q67 were as follows: the order of toxicity of the four pesticides to the luminescent bacteria Q67 was: the toxicity of the four pesticides was polyguodine (EC50 / 5. 35) Xicaojing (pEC5050 / 3.56) Metalaxelin (pEC50EC50 / 3.18) propoxapine (EC503.00), among which the toxicity of Doguodine was the greatest and propoxur was the most important. The toxicity of Metalaxyl and Xicaojing to Q67 was similar. The toxicity of seven rays to Q67 (EC50 value) has a good positive correlation with the concentration ratio of Xichaojing and Doguodine, especially the concentration ratio of Xicaojing to Doguodine, but it has a good positive correlation with propoxur and Doguodine, but it has a good positive correlation with the sum of the concentrations of Xicaojing and Doguodine. There was a good negative correlation between the concentration ratio and the concentration ratio of methylaniline. The model of RX 0.8729 (RMSE) 0.076C can be used to evaluate the toxicity of four pesticide quaternary mixtures to Q67. That is, the mixture system has a classical additive effect.
【作者單位】： 安徽建筑大學(xué)環(huán)境與能源工程學(xué)院安徽省水污染控制與廢水資源化重點(diǎn)實(shí)驗(yàn)室;清華大學(xué)新興有機(jī)污染物控制北京市重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(21677001,21207002) 安徽省自然科學(xué)基金(1708085MB50) 安徽省高等學(xué)校自然科學(xué)研究項(xiàng)目(KJ2013A067)共同資助
【分類號(hào)】：X171.5

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