本文選題：菲 + 反硝化降解��； 參考：《環(huán)境科學(xué)學(xué)報》2017年11期

【摘要】：從潛在多環(huán)芳烴(Polycyclic Aromatic Hydrocarbons,PAHs)污染的油田區(qū)域采集土壤樣品,以菲為唯一碳源且添加硝酸根的培養(yǎng)基來富集土壤中的菲反硝化降解菌群.隨后,通過定量PCR(Polymerase Chain Reaction)測定了獲取的富集菌群中反硝化相關(guān)功能基因(硝酸還原酶基因nar G、亞硝酸還原酶基因nir S)的豐度,并通過Illumina Mi Seq測序?qū)ζ渲械募毦郝浣Y(jié)構(gòu)進行解析.結(jié)果表明,獲取到的3個菌群(PDN-1、PDN-2和PDN-3)12 d內(nèi)對菲的降解率分別為45.18%、34.04%和25.92%.各富集培養(yǎng)菌群中nar G的豐度均高于nir S,且菲降解率最高的PDN-1中的反硝化相關(guān)基因豐度較低.Illumina Mi Seq測序結(jié)果表明,菲降解率最高的富集菌群PDN-1同時也具有較高的細菌多樣性指數(shù),變形菌門(Proteobacteria)、疣微菌門(Verrucomicrobia)和擬桿菌門(Bacteroidetes)是各富集菌群中的優(yōu)勢菌門,且Proteobacteria在3個富集菌群PDN-1(97.78%)、PDN-2(96.57%)、PDN-3(93.90%)中的比例均最高.變形菌門的Pseudomonas(γ-Proteobacteria)和Methylophilus(β-Proteobacteria)則是各富集菌群中最大的優(yōu)勢菌屬,前者為公認的PAHs降解菌,而后者則為能夠利用還原型"一碳化合物"的特殊菌屬.細菌多樣性與菲的降解率呈正相關(guān),表明菲的反硝化降解可能是多種細菌參與的共同結(jié)果.上述結(jié)果可為揭示典型PAHs反硝化降解的微生物機制提供理論依據(jù),同時為深入研究反硝化與菲代謝的偶聯(lián)機理打下基礎(chǔ).
[Abstract]:Soil samples were collected from potential polycyclic Aromatic hydrocarbonates (PAHs) contaminated oil fields. Phenanthrene was used as the sole carbon source and nitrate was added to enrich the phenanthrene denitrification bacteria community in the soil. Then, the abundance of denitrification-related functional genes (nar G and nir S) were determined by quantitative PCR(Polymerase Chain reaction, and the community structure was analyzed by Illumina Mi Seq sequencing. The results showed that the degradation rates of phenanthrene in PDN-1PDN-2 and PDN-3)12 d were 45.18% and 25.92%, respectively. The abundance of nar G in all enriched cultures was higher than that of nir, and the denitrification-related gene abundance in PDN-1 with the highest phenanthrene degradation rate was lower. Illumina Mi Seq sequencing results showed that, The enriched PDN-1 with the highest phenanthrene degradation rate also had a high bacterial diversity index. Proteobacteria, Verrucomicrobia, Verrucomicrobia and Bacteroidetes) were the dominant bacteria in each enriched flora, and Proteobacteria had the highest proportion among the three enriched bacteria PDN-197.787.757. Pseudomonas (緯 -Proteobacteria) and Methylophilus (尾 -Proteobacteria) are the most dominant bacteria in each enriched flora. The former is recognized as PAHs degrading bacteria, while the latter is a special genus which can utilize reduced carbon compounds. The diversity of bacteria was positively correlated with the degradation rate of phenanthrene, indicating that denitrification of phenanthrene might be the common result of many bacteria involved. These results can provide a theoretical basis for revealing the microbial mechanism of typical denitrification degradation of PAHs and lay a foundation for further study on the coupling mechanism between denitrification and phenanthrene metabolism.
【作者單位】： 西南大學(xué)資源環(huán)境學(xué)院;
【基金】：國家自然科學(xué)基金(No.41371477) 中央高校基本科研業(yè)務(wù)費專項(No.XDJK2014B047) 西南大學(xué)光炯創(chuàng)新實驗項目(No.2016012)~~
【分類號】：X172;X53
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本文編號：1883397