本文選題：篩選 + 污水��； 參考：《農(nóng)業(yè)工程學(xué)報》2017年20期

【摘要】：分離高效絮凝劑產(chǎn)生菌以有效絮凝豬場污水中的懸浮物。通過稀釋平板法和篩選培養(yǎng)基及以發(fā)酵液對豬場污水的絮凝率為指標復(fù)篩,從豬場污水沉淀池污泥中篩選獲得一株產(chǎn)絮凝劑的細菌FD-14,并進行16S r DNA鑒定,然后以絮凝效果為標準,采用La(34)正交設(shè)計對其培養(yǎng)條件進行優(yōu)化;采用單因素試驗對其碳氮源進行優(yōu)化。以最優(yōu)培養(yǎng)條件下菌株的發(fā)酵液實際絮凝豬場污水,與化學(xué)絮凝劑的絮凝效果進行比較。分離得到一株高效絮凝劑產(chǎn)生菌FD-14,分子生物學(xué)鑒定為解淀粉芽孢桿菌(Bacillus amyloliquefaciens),其產(chǎn)絮凝劑最適條件為溫度33℃,p H值6.6,轉(zhuǎn)速150 r/min,培養(yǎng)時間42 h。菌株FD-14利用淀粉作為其廉價碳源替代發(fā)酵培養(yǎng)基的絮凝率為80.80%,與原標準培養(yǎng)基差異不顯著(P0.05);對豬場實際應(yīng)用結(jié)果表明,微生物絮凝劑和化學(xué)絮凝劑對豬場污水絮凝率分別為79.0%和62.7%,微生物絮凝劑比化學(xué)絮凝劑對豬場污水絮凝率高16.3%。解淀粉芽孢桿菌FD-14是生物絮凝劑高效產(chǎn)生菌,其產(chǎn)生的絮凝劑可用于豬場污水懸浮物絮凝。
[Abstract]:High-efficiency flocculant-producing bacteria were isolated to effectively flocculate suspended matter from pig farm wastewater. A flocculant producing bacteria FD-14 was obtained by dilution plate method and screening medium, and the flocculation rate of fermentation broth on pig farm sewage was rescreened. A bacterial strain FD-14 was obtained from the sludge of pig farm sewage sedimentation tank, and the 16s r DNA was used to identify the flocculant. Then the culture conditions were optimized by La-34 orthogonal design, and the carbon and nitrogen sources were optimized by single factor test. Under the optimal culture conditions, the actual flocculation of pig wastewater by fermentation broth was compared with that of chemical flocculant. An efficient flocculant producing strain FD-14 was isolated and identified as Bacillus amyloliquefaciensus by molecular biology. The optimum conditions for flocculant production were as follows: temperature 33 鈩，

本文編號：1829554