本文選題：石油污染土壤　切入點：模式線蟲　出處：《環(huán)境科學(xué)學(xué)報》2017年11期

【摘要】：隨著經(jīng)濟快速發(fā)展,石油及其產(chǎn)品用量增多,石油污染問題日益嚴(yán)重,土壤石油污染治理刻不容緩.研究表明石油污染土壤中存在大量的食細菌線蟲,但食細菌線蟲在石油污染土壤中的作用還不清楚.本試驗采用人工模擬石油污染土壤,通過接種不同密度模式線蟲(Caenorhabditis elegans),研究食細菌線蟲在石油污染土壤中的功能及其對污染土壤中石油降解及土壤微生物活性的影響.本實驗共設(shè)6個處理:高溫滅菌石油污染土壤(FSP),作為對照處理1;殺滅線蟲土壤(S),作為對照處理2;石油污染土壤(SP);石油污染土壤+5條線蟲/g干土(SPN5);石油污染土壤+10條線蟲/g干土(SPN10);石油污染土壤+20條線蟲/g干土(SPN20).研究結(jié)果表明:整個試驗培養(yǎng)結(jié)束時,處理SP、SPN5、SPN10和SPN20的石油殘留量比第0天采樣時分別降低約60.78%、80.01%、67.63%和66.31%,處理SP、SPN5、SPN10和SPN20的石油殘留量比處理FSP分別降低約43.60%、70.68%、52.34%和50.45%,得出接種線蟲可以促進污染土壤的石油降解,其中接種5條線蟲/g干土的處理促進石油降解效果最好.第7 d采樣時,處理SP、SPN5、SPN10、SPN20中脫氫酶酶活性比處理S分別增加約132.76%、115.09%、118.67%和55.81%,表明石油污染可以激活脫氫酶;第14 d時,接種線蟲處理SPN5、SPN10和SPN20的脫氫酶活性比未接種線蟲處理SP的脫氫酶活性分別增加約5.16%、18.13%和29.56%,表明添加食細菌線蟲也促進了土壤相關(guān)酶活性.該研究證明食細菌線蟲可以在石油污染土壤中刺激微生物的繁殖,增強土壤酶活性,進而促進污染土壤石油的降解.
[Abstract]:With the rapid development of economy, the consumption of petroleum and its products is increasing, the problem of oil pollution is becoming more and more serious, and the treatment of soil oil pollution is urgent. Research shows that there are a large number of bacteriophagous nematode in oil-contaminated soil. However, the role of bacterial nematode in petroleum contaminated soil is not clear. The effects of bacterial nematode on petroleum degradation and microbial activity in petroleum contaminated soil were studied by inoculating Caenorhabditis eleanss with different densities. Six treatments were established in this experiment: high temperature sterilization. Petroleum contaminated soil FSPN, treated as control 1; nematode killing soil, 2 as control; petroleum contaminated soil, SPN; petroleum contaminated soil, 5 nematodes / g dry soil SPN5; petroleum contaminated soil, 10 nematodes / g dry soil, SPN10; petroleum contaminated soil. Soil 20 nematodes / g dry soil spN20. The results showed that: at the end of the whole experiment, The petroleum residues of SPN5 SPN10 and SPN20 treated with SPN5 and SPN20 were about 60.78% and 66.31% lower than those of day 0, respectively. The petroleum residues of SPN5SPN10 and SPN20 were reduced by 43.6070.6852.34% and 50.4545% than that of FSP, respectively. The results showed that inoculation of nematode could promote the oil degradation of contaminated soil. The treatment of inoculating 5 nematodes / g dry soil had the best effect on oil degradation. At the 7th day of sampling, the dehydrogenase activity in SPN5 / 10 SPN10 SPN20 was increased by 132.76% and 55.81%, respectively, compared with that of S, indicating that oil pollution can activate dehydrogenase; on the 14th day, The dehydrogenase activities of SPN5, SPN10 and SPN20 inoculated with nematodes increased by about 5.16% and 29.56%, respectively, compared with those of non-inoculated nematodes, which indicated that the addition of bacterial nematode also promoted the activity of soil related enzymes. To stimulate the propagation of microbes in petroleum-contaminated soils, Enhance soil enzyme activity, and then promote the degradation of contaminated soil oil.
【作者單位】： 安徽農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;南昌工程學(xué)院江西省退化生態(tài)系統(tǒng)修復(fù)與流域生態(tài)水文重點實驗室;
【基金】：江西省教育廳科學(xué)技術(shù)研究項目(No.GJJ20151118) 國家自然科學(xué)基金(No.31460149) 江西省科技廳重點研發(fā)計劃項目(No.20161BBG70050) 安徽省生物學(xué)重點學(xué)科項目(No.2014QJSK015)~~
【分類號】：X53

【相似文獻】

相關(guān)期刊論文 前9條

1 加村崇雄;王家玉;;土壤微生物活性的測定 Ⅸ、鐵錳代謝[J];土壤學(xué)進展;1979年02期

2 JohnE;Roger;鐘瓊積;;金屬等無機離子對土壤微生物活性的影響[J];農(nóng)業(yè)環(huán)境與發(fā)展;1987年03期

3 H.F.STROO;邵鳳君;;8710下水污泥對土壤微生物活性的影響[J];農(nóng)業(yè)環(huán)境與發(fā)展;1987年02期

4 E.Schuster;王孝堂;;農(nóng)藥處理系統(tǒng)對土壤微生物活性的影響[J];土壤學(xué)進展;1988年01期

5 朱南文,胡茂林,高廷耀;甲胺磷對土壤微生物活性的影響[J];農(nóng)業(yè)環(huán)境保護;1999年01期

6 M.Waingwright;吳文禮;;農(nóng)藥對土壤微生物活性的影響[J];土壤學(xué)進展;1980年04期

7 侯憲文;吳建軍;徐建明;;鉛-芐嘧磺隆對土壤微生物活性與群落結(jié)構(gòu)的影響[J];中國環(huán)境科學(xué);2007年06期

8 王麗超;羅學(xué)剛;彭芳芳;趙魯雪;;鈾尾礦污染土壤微生物活性及群落功能多樣性變化[J];環(huán)境科學(xué)與技術(shù);2014年03期

9 陸引罡,黃建國,滕應(yīng),羅永水,王榮平,湛方棟;重金屬污染黃壤生態(tài)修復(fù)中的微生態(tài)效應(yīng)[J];水土保持學(xué)報;2004年05期

相關(guān)碩士學(xué)位論文 前1條

1 阮菊俊;銅污染對土壤微生物活性的影響及其PCR-DGGE分析[D];揚州大學(xué);2008年

，

本文編號：1654393