本文選題：酶解木薯渣　切入點(diǎn)：生物絮團(tuán)　出處：《廣西大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：為解決目前生物絮團(tuán)技術(shù)碳源成本過(guò)高以及由于木薯渣處理困難造成的環(huán)境問(wèn)題,本文探究了酶解木薯渣作為生物絮團(tuán)碳源的可行性。首先本文優(yōu)化了酶解木薯渣的工藝流程,然后將最佳反應(yīng)條件下得到的酶解產(chǎn)物與原木薯渣、蔗糖作為三種不同的碳源添加進(jìn)南美白對(duì)蝦養(yǎng)殖水體中,最后通過(guò)水質(zhì)分析,判斷蔗糖、木薯渣和酶解木薯渣三種碳源對(duì)水質(zhì)影響的區(qū)別,并利用高通量測(cè)序技術(shù)對(duì)生物絮團(tuán)和對(duì)蝦腸道中的微生物群落結(jié)構(gòu)進(jìn)行解析。本研究對(duì)高效利用木薯渣,解決其造成的環(huán)境問(wèn)題具有重要意義,也為解釋生物絮團(tuán)的作用機(jī)理提供了理論參考。主要結(jié)果歸納如下:(1)確定了纖維素酶、糖化酶和α-淀粉酶單獨(dú)水解木薯渣的最佳pH、反應(yīng)溫度、酶添加量、固液比和最佳反應(yīng)時(shí)間。(2)確定了當(dāng)纖維素酶、糖化酶和α-淀粉酶添加量分別為8000 U/g木薯渣、4000 U/g木薯渣和640 U/g木薯渣,反應(yīng)時(shí)間分別為48 h、10 h和40 h時(shí),三種酶復(fù)合作用的最佳效果比單一酶種最佳的作用效果提高了97.92%,同時(shí)該結(jié)果明顯高于理論上三種酶解效果之和。(3)取預(yù)處理過(guò)的木薯渣,分別調(diào)節(jié)固液比為1/15、1/10、1/5,并在不同條件下依次添加七種生物酶,最終得到的還原糖的濃度分別34.75 mg/mL、50.55 mg/mL、92.32mg/mL和,相較于未經(jīng)處理的木薯渣,還原糖提取率提高了 20倍。(4)在周期為30天的養(yǎng)殖實(shí)驗(yàn)中,分別將蔗糖、木薯渣、酶解木薯渣添加到對(duì)蝦養(yǎng)殖桶中,水質(zhì)分析表明,蔗糖組的氨氮濃度(0.16 mg/L)和亞硝態(tài)氮濃度(0.13 mg/L)相較于木薯渣組分別降低了 23.8%和27.8%,酶解木薯渣組的氨氮濃度(0.19 mg/L)和亞硝態(tài)氮濃度(0.16 m/L)分別降低了 9.5%和12.5%,TSS(總固體懸浮物濃度)和BFV(生物絮團(tuán)體積濃度)水平最高的組為酶解木薯渣組。蔗糖在控制氨氮、亞硝態(tài)氮兩種污染指標(biāo)方面效果最好,其次是酶解木薯渣,但是在生物絮團(tuán)的形成方面,酶解木薯渣的處理效果最好。(5)高通量測(cè)序結(jié)果表明,生物絮團(tuán)中的微生物多樣性遠(yuǎn)高于對(duì)蝦腸道,BFT3(碳源為酶解木薯渣的生物絮團(tuán))中異養(yǎng)細(xì)菌的相對(duì)豐度分別比BFT1(碳源為蔗糖的生物絮團(tuán))和BFT2(碳源為木薯渣的生物絮團(tuán))高了 20.70%和和1.19%,同時(shí)其益生菌的相對(duì)豐度比BFT1和BFT2高了 11.47%和61.60%,來(lái)自于BFT3水環(huán)境培養(yǎng)的對(duì)蝦腸道樣品中,致病菌所占的比例低于其他兩組,說(shuō)明了酶解木薯渣的添加提高了益生菌和異養(yǎng)細(xì)菌并且抑制了致病菌來(lái)降低對(duì)蝦的疾病風(fēng)險(xiǎn)。
[Abstract]:In order to solve the environmental problems caused by the high cost of carbon source and the difficulty of cassava residue treatment, the feasibility of enzymatic cassava residue as a carbon source of biological floc was explored. Firstly, the technological process of enzymatic cassava residue was optimized. The enzymatic hydrolysis products and cassava dregs, sucrose as three different carbon sources were added to the culture water of Penaeus vannamei under the best reaction conditions. Finally, the sucrose was judged by the analysis of water quality. The effects of cassava residue and enzymatic cassava residue on water quality were studied. The microbial community structure in biological flocs and shrimp intestines was analyzed by high-throughput sequencing. It is of great significance to solve the environmental problems caused by it, and also provides a theoretical reference for explaining the action mechanism of biological flocs. The main results are summarized as follows: 1) Cellulase is determined. The optimum pH, reaction temperature, enzyme addition, solid-liquid ratio and optimum reaction time of cassava residue hydrolyzed separately by Glucoamylase and 偽 -amylase were determined. The dosages of Glucoamylase and 偽 -amylase were 8 000 U / g cassava dregs and 640 U / g cassava dregs respectively, and the reaction time was 48 h / 10 h and 40 h respectively. The optimum effect of the three enzymes was 97.922.The result was significantly higher than that of cassava dregs pretreated with the sum of the three enzymatic hydrolysis effects in theory, and the optimum effect of the three enzymes was higher than that of the single enzyme, and the result was significantly higher than that of the pre-treated cassava dregs. The ratio of solid to liquid was adjusted to 1 / 15 / 1 / 10 / 1 / 5, and seven kinds of biological enzymes were added under different conditions, and the final concentration of reducing sugar was 34.75 mg / mL, 50.55 mg / mL and 92.32 mg / mL, respectively, compared with the untreated cassava residue. The extraction rate of reducing sugar was increased by 20 times. 4) in the culture experiment with a period of 30 days, sucrose, cassava residue and cassava residue were added to the shrimp culture barrel respectively. The water quality was analyzed. The ammonia concentration of sucrose group (0.16 mg / L) and nitrite concentration (0.13 mg / L) decreased by 23.8% and 27.8 respectively compared with cassava dregs, the ammonia concentration of enzymatic cassava dregs was 0.19 mg / L and nitrite concentration was 0.16 mg / L and 9.5% and 12.5 mg / L, respectively. The highest concentration of floatation) and BFV (volume concentration of biological flocs) were obtained from cassava dregs by enzymatic hydrolysis. Sucrose was used to control ammonia nitrogen. The effect of nitrite nitrogen on two pollution indexes was the best, followed by enzymatic cassava residue, but in the formation of biological flocs, the treatment effect of enzymatic cassava residue was the best. The microbial diversity in the biological flocs is much higher than that in the shrimp intestinal tract BFT3 (where the carbon source is enzymatic cassava residue) and the relative abundance of heterotrophic bacteria is higher than that of BFT1 (carbon source is sucrose biological flocs) and BFT2 (carbon source is cassava residue). At the same time, the relative abundance of probiotics was 11.47% and 61.60 higher than that of BFT1 and BFT2. The proportion of pathogenic bacteria was lower than that of the other two groups, which indicated that the addition of cassava residue could increase probiotics and heterotrophic bacteria and inhibit pathogenic bacteria to reduce the risk of shrimp disease.
【學(xué)位授予單位】：廣西大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：X714

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 熊素敏,左秀鳳,朱永義;稻殼中纖維素、半纖維素和木質(zhì)素的測(cè)定[J];糧食與飼料工業(yè);2005年08期

2 浦躍武;劉堅(jiān);;木薯渣厭氧發(fā)酵制取沼氣的研究[J];安徽農(nóng)業(yè)科學(xué);2009年29期

3 方詡;秦玉琪;李雪芝;王祿山;汪天虹;朱明田;曲音波;;纖維素酶與木質(zhì)纖維素生物降解轉(zhuǎn)化的研究進(jìn)展[J];生物工程學(xué)報(bào);2010年07期

4 羅國(guó)芝;朱澤聞;潘云峰;魯璐;譚洪新;;生物絮凝技術(shù)在水產(chǎn)養(yǎng)殖中的應(yīng)用[J];中國(guó)水產(chǎn);2010年02期

5 方佳;濮文輝;張慧堅(jiān);;國(guó)內(nèi)外木薯產(chǎn)業(yè)發(fā)展近況[J];中國(guó)農(nóng)學(xué)通報(bào);2010年16期

6 容元平;廖蘭;伍時(shí)華;葉云;李克林;;木薯渣殘余淀粉提取與發(fā)酵酒精工藝研究[J];安徽農(nóng)業(yè)科學(xué);2010年06期

7 夏耘;郁二蒙;謝駿;余德光;王廣軍;李志斐;王海英;龔?fù)麑?;基于PCR-DGGE技術(shù)分析生物絮團(tuán)的細(xì)菌群落結(jié)構(gòu)[J];水產(chǎn)學(xué)報(bào);2012年10期

8 黃萍;齊仁立;;木薯渣作為飼料資源的開(kāi)發(fā)與利用[J];四川畜牧獸醫(yī);2012年12期

9 黃春芳;朱玉連;莫虎明;孔巧平;古碧;周敬紅;;不同預(yù)處理方法對(duì)木薯渣水解效果影響的研究[J];廣西大學(xué)學(xué)報(bào)(自然科學(xué)版);2013年02期

10 楊婷;廖美德;劉Pr嘉;賀玉廣;吳杰良;;木薯渣混菌發(fā)酵替代麩皮飼料的研究[J];飼料博覽;2014年11期

相關(guān)博士學(xué)位論文 前1條

1 徐武杰;生物絮團(tuán)在對(duì)蝦零水交換養(yǎng)殖系統(tǒng)中功能效應(yīng)的研究與應(yīng)用[D];中國(guó)海洋大學(xué);2014年

相關(guān)碩士學(xué)位論文 前6條

1 陳倩伶;生物絮團(tuán)技術(shù)應(yīng)用于對(duì)蝦養(yǎng)殖水質(zhì)調(diào)控[D];廣西大學(xué);2015年

2 傅超英;木薯渣纖維素和淀粉的糖化及其發(fā)酵丁醇的研究[D];華南理工大學(xué);2014年

3 趙大虎;添加不同碳源對(duì)生物絮團(tuán)組分和凡納濱對(duì)蝦生理健康、生長(zhǎng)的影響[D];中國(guó)海洋大學(xué);2013年

4 龔信芳;木薯渣異步糖化發(fā)酵和同步糖化發(fā)酵產(chǎn)乙醇的研究[D];華南理工大學(xué);2011年

5 劉傳都;利用混合菌固體發(fā)酵木薯渣生產(chǎn)菌體蛋白飼料的研究[D];華中農(nóng)業(yè)大學(xué);2009年

6 賴翠華;木薯淀粉渣制取單細(xì)胞蛋白飼料的實(shí)驗(yàn)研究[D];廣西大學(xué);2006年

，

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