本文選題：SBR系統(tǒng)　切入點(diǎn)：土霉素　出處：《浙江工商大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：近年來,大量的土霉素(OTC)已被應(yīng)用于醫(yī)藥以及畜牧業(yè)。由于土霉素只有少部分能夠被生物降解,因此大量土霉素會(huì)排入至水環(huán)境中。污水中存在的土霉素可能會(huì)對生物污水處理系統(tǒng)中的微生物(尤其是功能性微生物如硝化菌和反硝化菌等)產(chǎn)生不良影響,從而導(dǎo)致生物污水處理系統(tǒng)的污水處理效果不理想。因此,研究土霉素對污水處理廠中生化過程的影響是十分必要的。本試驗(yàn)接種污泥為杭州市七格污水處理廠曝氣池出口污泥,經(jīng)過實(shí)驗(yàn)室馴化,SBR系統(tǒng)脫氮效率穩(wěn)定在95.0%以上,去除CODcr效果顯著。本文基于SBR系統(tǒng),從脫氮過程、化學(xué)需氧量(CODcr)的去除過程、胞外聚合物(EPS)的總量變化過程、比呼吸速率(SOUR)的變化過程以及微生物群落的變化等多個(gè)角度出發(fā),較系統(tǒng)地研究了不同濃度土霉素對SBR系統(tǒng)的沖擊作用。結(jié)果表明,由于土霉素的作用,系統(tǒng)的氨氮去除率從99.0%分別下降至77.2%(1 mg L-1)、47.4%(5 mg L-1)以及6.66%(10 mg L-1),在30天抑制試驗(yàn)之后的15天恢復(fù)階段內(nèi),R2(mgL-1)、R3(5mgL-1)反應(yīng)器具有較好恢復(fù)性,而R4(10 mg L-1)反應(yīng)器恢復(fù)性較差。缺氧段CODcr去除率從89.51%分別下降至 84.55%(1 mg L-1)、16.23%(5 mg L-1)以及 2.51%(10 mg L-1),在恢復(fù)階段出現(xiàn)不同程度恢復(fù),但無法恢復(fù)至初始水平。此外,高濃度土霉素(10 mg L-1)引起EPS分泌量和異養(yǎng)菌SOUR分別下降至空白組的44.3%和41.2%。由高通量分析可得,由于高濃度土霉素(5mgL-1以上)的作用,活性污泥中微生物的多樣性顯著下降,且微生物的群落結(jié)構(gòu)也發(fā)生顯著變化。系統(tǒng)中的微生物的變形菌門比例不斷下降,而擬桿菌門比例出現(xiàn)上升,該發(fā)現(xiàn)有助于后續(xù)相關(guān)試驗(yàn)對某些特定菌種的篩選作用。屬層面的分析可得,高濃度土霉素(5mgL-1以上)反應(yīng)器中的部分氨氧化菌,例如Nitrospira和Nitrosomonas均受到了抑制;缺氧異養(yǎng)菌聚糖菌(Candidatus_Competibacter)比例下降明顯,與宏觀數(shù)據(jù)氨氮和CODCr去除效率下降相符。菌膠團(tuán)Zoogloea比例上升,發(fā)生非絲狀菌膨脹。
[Abstract]:In recent years, a large number of oxytetracycline (OTC) have been used in medicine and animal husbandry, because only a small part of oxytetracycline can be biodegraded. Therefore, a large amount of oxytetracycline is discharged into the water environment. The presence of oxytetracycline in sewage may have a negative effect on microorganisms in the biological wastewater treatment system (especially functional microorganisms such as nitrifying bacteria and denitrifying bacteria, etc.). As a result, the sewage treatment effect of the biological sewage treatment system is not ideal. It is necessary to study the effect of oxytetracycline on the biochemical process in wastewater treatment plant. The sludge inoculated in this experiment is the outlet sludge of the aeration tank of the seven wastewater treatment plants in Hangzhou. The denitrification efficiency of the system is stable at more than 95.0% after the laboratory acclimation. The removal effect of CODcr is remarkable. Based on the SBR system, this paper starts from the denitrification process, the removal process of chemical oxygen demand (COD), the total change process of extracellular polymer (CODcr), the change process of specific respiration rate (CODcr), and the change of microbial community, etc. The impact of different concentrations of oxytetracycline on SBR system was systematically studied. The removal rate of ammonia nitrogen in the system decreased from 99.0% to 77.2 mg L -1, 47.44 mg L -1 and 6.66 mg L -1, respectively. During the 15-day recovery period after the 30-day inhibition test, the R2mgL-1 / R3GL-1) reactor had a good recovery, and the total ammonia nitrogen removal rate decreased from 99.0% to 77.2mgL-1, 47.4mg / L, 47.4mg / L and 6.66mg / L ~ (-1), respectively. During the 15-day recovery period after the 30-day inhibition experiment, the reactor was of good restorability. The CODcr removal rate of the anoxic stage decreased from 89.51% to 84.551mg / L and 16.23235mg / L, respectively, and the recovery of 10mg / L ~ (-1) and 2.51g / L ~ (10 mg / L ~ (-1)) showed different degrees of recovery, but could not recover to the initial level. High concentration oxytetracycline (10 mgL-1) reduced the secretion of EPS and SOUR of heterotrophic bacteria to 44.3% and 41.2in blank group, respectively. By high-throughput analysis, the microbial diversity in activated sludge decreased significantly due to the effect of high concentration of oxytetracycline (> 5 mg 路L ~ (-1)). And the community structure of microorganism also changed significantly. The proportion of Proteus phylum of microorganism in the system decreased continuously, but the proportion of Bacteroides increased. This finding is helpful to the screening of certain strains in the subsequent related tests. At the generic level, some ammonia-oxidizing bacteria, such as Nitrospira and Nitrosomonas, were inhibited in the reactor with high concentration of oxytetracycline (> 5 mg 路L ~ (-1)). The proportion of anoxic heterotrophic bacteria Candidatus Competitive bacteria decreased obviously, which was consistent with the decrease of the removal efficiency of ammonia nitrogen and CODCr in macroscopical data. The ratio of Zoogloea of micelle increased and the expansion of non-filamentous bacteria occurred.
【學(xué)位授予單位】：浙江工商大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：X703

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