本文關(guān)鍵詞：克雷伯氏菌脫色偶氮染料廢水的生化機(jī)理研究　出處：《蘇州科技大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 蒽醌-2-磺酸鈉 脫色 克雷伯氏菌 產(chǎn)能 產(chǎn)氫

【摘要】：偶氮染料廣泛的應(yīng)用于紡織、印染行業(yè),其是一類(lèi)含有偶氮鍵(—N=N—)和芳香基等有機(jī)化合物的典型染料;其中作為發(fā)色基團(tuán)的偶氮鍵決定著廢水的顏色,在其印染過(guò)程由于著色不完全產(chǎn)生的染料廢水對(duì)動(dòng)植物和人類(lèi)均存在極大的危害;目前,吸附法、生物法和高級(jí)氧化法是偶氮染料廢水應(yīng)用較多的處理方法。高級(jí)氧化法多集中于·OH、·SO_4~(2-)催化劑的研究,生物法多集中于污泥法、新型細(xì)菌發(fā)酵研究等,吸附法對(duì)于新型碳質(zhì)材料和合適的催化劑的研究較多,研究者們希望通過(guò)發(fā)現(xiàn)更多效果更好的催化劑和碳質(zhì)材料以促進(jìn)偶氮染料廢水的脫色。相較于吸附法和高級(jí)氧化法的處理能力有限、耗能高,生物法脫色徹底、耗能低、產(chǎn)生的污染少,正逐漸成為近年來(lái)偶氮染料廢水研究的熱點(diǎn)。其中,微生物處理法因?yàn)榘怆娮哟┧篌w的添加可以帶來(lái)經(jīng)濟(jì)、高效的處理效果而備受人們的關(guān)注。其中,蒽氫醌-2,6-二磺酸鈉(AHQDS)、蒽醌-2-磺酸鈉(AQS)、腐殖酸(HA)作為醌類(lèi)物質(zhì)可以在細(xì)菌厭氧發(fā)酵脫色偶氮染料的過(guò)程中起到促進(jìn)作用,這類(lèi)物質(zhì)可作為細(xì)菌厭氧發(fā)酵過(guò)程中的電子穿梭體,加快細(xì)菌代謝過(guò)程中的電子傳遞。除此之外,活性碳也可以作為催化劑促進(jìn)染料分子的去除,論文中選擇Klebsiella oxytoca GS-4-08作為脫色偶氮染料甲基橙的主體,考察了單獨(dú)的活性炭活性炭與克雷伯氏菌復(fù)合,腐殖酸負(fù)載鐵與細(xì)菌復(fù)合,以及AQS對(duì)偶氮染料的去除效果;也通過(guò)考察細(xì)菌對(duì)于AQS、GAC、Fe-HA的耐受性,選擇AQS作為最佳電子穿梭體,考察其對(duì)于Klebsiella oxytoca GS-4-08厭氧發(fā)酵過(guò)程的影響。主要研究?jī)?nèi)容和結(jié)論如下:(1)在實(shí)驗(yàn)室的條件中,配置1.5g·L~(-1) HA,10000g離心20min去除不溶物,加入5 mmol·L~(-1) FeSO_4·7H_2O,快速攪拌,調(diào)節(jié)Ph=7,將懸浮液靜置一周,再重復(fù)上述離心過(guò)程,收集沉淀物進(jìn)行冷凍干燥,獲得Fe-HA沉淀物,對(duì)Fe-HA與Klebsiella oxytoca GS-4-08復(fù)合降解偶氮染料甲基橙進(jìn)行考察分析,發(fā)現(xiàn)在30h時(shí),細(xì)菌在空白、Fe、FeHA復(fù)合情況下脫色甲基橙分別可以達(dá)到62%、11%、52%,說(shuō)明Fe-HA對(duì)細(xì)菌的耐受性有一定的影響。(2)通過(guò)控制GAC的濃度,考察其對(duì)偶氮染料甲基橙的去除效果,結(jié)果發(fā)現(xiàn)在2、5、8、10 g·L~(-1)時(shí),甲基橙的降解過(guò)程可以很好的與一級(jí)降解動(dòng)力學(xué)方程擬合;甲基橙脫色效率隨著GAC濃度的增加而隨之增加,在2h內(nèi)10g·L~(-1)GAC可脫色99%的甲基橙,8g·L~(-1) GAC可以在2.75h內(nèi)脫色99%的甲基橙,5g·L~(-1)GAC可以在5h內(nèi)脫色99%的甲基橙,2g·L~(-1)GAC可以在10.5h內(nèi)脫色97%的甲基橙;2g·L~(-1)GAC與細(xì)菌復(fù)合前后脫色甲基橙的降解情況均符合二級(jí)降解動(dòng)力學(xué)方程,而其脫色速率分別為0.175、0.17,兩者差別不大,而在實(shí)驗(yàn)反應(yīng)10h后,GAC與菌株復(fù)合反應(yīng)的實(shí)驗(yàn)中出現(xiàn)菌株死亡現(xiàn)象,這說(shuō)明GAC會(huì)影響菌株脫色甲基橙的耐受性。(3)通過(guò)投加AQS進(jìn)入細(xì)菌厭氧發(fā)酵過(guò)程中,發(fā)現(xiàn)在未加AQS的情況下,25h細(xì)菌脫色MO只能達(dá)到60%,而0.08mmol·L~(-1)的AQS可以在10h內(nèi)脫色MO達(dá)100%,且在AQS參與的細(xì)菌厭氧發(fā)酵過(guò)程中,菌株生長(zhǎng)狀況良好,且在厭氧發(fā)酵后期出現(xiàn)產(chǎn)氫情況,所以論文后期將重點(diǎn)放在AQS促進(jìn)Klebsiella oxytoca GS-4-08厭氧發(fā)酵產(chǎn)氫上。(4)考察不同AQS濃度下,Klebsiella oxytoca GS-4-08利用蔗糖厭氧發(fā)酵產(chǎn)氫脫色甲基橙過(guò)程中,分析了甲基橙的脫色動(dòng)力學(xué)、產(chǎn)氫動(dòng)力學(xué)、蔗糖降解動(dòng)力學(xué)、以及細(xì)菌生長(zhǎng)情況,以及在厭氧發(fā)酵過(guò)程中,嗜溫菌Klebsiella oxytoca GS-4-08作為單一菌株厭氧發(fā)酵,AQS作為電子穿梭體改變電子傳遞速率,對(duì)電子平衡和能源轉(zhuǎn)換的影響。結(jié)果發(fā)現(xiàn):最佳濃度為0.1 mmol·L~(-1)。在該濃度下,Klebsiella oxytoca GS-4-08可在10h內(nèi)完全脫色MO,25h內(nèi)降解蔗糖達(dá)到92%,同時(shí)產(chǎn)生23.3 mmol·L~(-1)乙醇和23.5mmol·L~(-1)乙酸,100.5 mL氫氣;AQS濃度的電子得率最高為90.5%;通過(guò)對(duì)比降解過(guò)程中能源的轉(zhuǎn)換,得到能源產(chǎn)量最高為802 kJ·mol蔗糖~(-1)以及Klebsiella oxytoca GS-4-08發(fā)酵過(guò)程可產(chǎn)生乙醇和氫氣兩種以上生物燃料。
[Abstract]:Azo dyes are widely used in textile, printing and dyeing industry, it is a kind of containing azo bond (N=N) typical dyes and aromatic organic compounds; the azo bond chromophore determines the color of wastewater in the dyeing process, due to incomplete colored dye wastewater has great harm the plants and animals and humans; at present, adsorption method, biological method and advanced oxidation process is a method of treating wastewater containing azo dye used. Advanced oxidation method focused on OH, SO_4~ (2-) catalysts, biological method focused on the research of new bacterial fermentation sludge, etc., there are many studies on adsorption method new carbonaceous material and a suitable catalyst, the researchers hope to promote the decolorization of azo dye wastewater through the discovery of more better catalysts and carbonaceous materials. Compared to the processing capacity of adsorption and advanced oxidation method Limited, high energy consumption, biological decolorization completely, low energy consumption, produce less pollution, it has become a hot research of azo dye wastewater in recent years. Among them, the microbial process for adding extracellular electron shuttle can bring economic, highly efficient processing and attracted much attention. Among them, anthrahydroquinone -2,6- two sodium sulfonate (AHQDS), anthraquinone -2- sulfonate (AQS), humic acid (HA) as quinones can play a role in the process of anaerobic fermentation of bacterial decolorization of azo dyes, this kind of material can be used as electronic anaerobic bacteria in the fermentation process of shuttle, accelerate the electron transfer during bacterial metabolism. In addition in addition, the activated carbon can be used as a catalyst to promote the removal of dye molecules, the choice of Klebsiella oxytoca GS-4-08 as the main body of the decolorization of azo dye methyl orange, the effect of carbon activated carbon alone with Klebsiella complex Together, humic acid and bacterial load of iron compound, and AQS of azo dye removal effect; also through the investigation of bacteria for AQS, GAC, Fe-HA tolerance, AQS was selected as the best electron shuttle, to investigate its effects on Klebsiella oxytoca GS-4-08 anaerobic fermentation process. The main research contents and conclusions are as follows: (1) in laboratory conditions, configuration of 1.5g L~ (-1) HA, 10000g 20min centrifugation to remove insoluble matter, adding 5 mmol - L~ (-1) FeSO_4 - 7H_2O, rapid mixing, adjusting Ph=7, suspension static one week, and then repeat the centrifugal process, the precipitate was collected by freeze drying, Fe-HA precipitates investigation and analysis of Fe-HA, Klebsiella and oxytoca GS-4-08 composite degradation of azo dye methyl orange, found in 30h, Fe, FeHA of bacteria in the blank, composite under the condition of decoloration of methyl orange can respectively reach 62%, 11%, 52%, indicating that tolerance to bacteria is Fe-HA The effect of (2). By controlling the concentration of GAC, the removal of azo dye methyl orange, were found in the 2,5,8,10 g L~ (-1), the degradation process of methyl orange can be very good with the first-order reaction kinetics equation; the decolorization efficiency increases with the increase of GAC concentration in 2H 10g L~ (-1) GAC 99% 8g, the decolorization of methyl orange, L~ (-1) GAC in 2.75h 99% 5g, the decolorization of methyl orange, L~ (-1) GAC in 5h 99% 2G, the decolorization of methyl orange, L~ (-1) GAC in 10.5h 97% 2G the decolorization of methyl orange; L~ (-1) and bacterial degradation of GAC composite before and after decolorization of methyl orange were consistent with two degradation kinetic equation, and the decolorization rate was 0.175,0.17, the difference is small, while in the experimental reaction after 10h strain death phenomenon of complex reaction GAC and strain experiment, indicating that the GAC strains will affect the decolorization of methyl The tolerance of orange. (3) by adding AQS into the bacterial anaerobic fermentation process, found in the case of AQS without 25h, the decolorization MO can reach 60%, and 0.08mmol L~ (-1) AQS in 10h MO was up to 100%, and the bacteria in the anaerobic fermentation process in AQS strain, good growth status, and hydrogen production in anaerobic fermentation stage, so at the end of the paper will focus on the promotion of AQS Klebsiella oxytoca GS-4-08 of fermentative hydrogen production. (4) the effects of different concentrations of AQS, Klebsiella oxytoca GS-4-08 using sucrose fermentative hydrogen production in the process of decolorization of methyl orange, methyl orange decolorization kinetics analysis hydrogen production, kinetics, sucrose degradation kinetics, and bacterial growth, and in the process of anaerobic digestion, mesophilic bacteria Klebsiella oxytoca GS-4-08 as a single strain of anaerobic fermentation, AQS as electron shuttle change electron transfer rate, Effect of electronic balance and energy conversion. The results showed that the optimum concentration of 0.1 mmol L~ (-1). The concentration of Klebsiella, oxytoca and GS-4-08 completely within the 10h MO 25h the decolorization, degradation of sucrose reached 92%, while producing 23.3 mmol - L~ (-1) and 23.5mmol L~ ethanol (-1) acetic acid 100.5, mL electronic hydrogen; AQS concentration was the highest rate of 90.5%; through the conversion of energy contrast in the degradation process, obtained the highest yield energy was 802 kJ - mol ~ (-1) and Klebsiella sucrose oxytoca GS-4-08 fermentation process of ethanol and hydrogen can produce more than two kinds of bio fuels.

【學(xué)位授予單位】：蘇州科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：X791

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 喻陽(yáng);鐘笑涵;徐愛(ài)華;夏東升;曾慶福;潘飛;;磁性O(shè)MS-2/SBR耦合處理活性染料X-3B廢水[J];環(huán)境工程學(xué)報(bào);2016年10期

2 鄒專(zhuān)勇;周建迪;楊艷秋;趙連英;繆宏超;朱丹萍;;生物質(zhì)活性炭制備關(guān)鍵技術(shù)與應(yīng)用現(xiàn)狀[J];成都紡織高等專(zhuān)科學(xué)校學(xué)報(bào);2016年02期

3 黃亞君;歐曉霞;胡友彪;;腐殖酸及其與金屬絡(luò)合物的光譜學(xué)表征[J];綠色科技;2016年04期

4 葉乃芳;王中新;;16S rRNA及相關(guān)技術(shù)用于臨床細(xì)菌鑒定的研究進(jìn)展[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2015年04期

5 盧婧;余志晟;張洪勛;;微生物降解偶氮染料的研究進(jìn)展[J];工業(yè)水處理;2013年01期

6 丁春生;曾海明;黃燕;彭芳;徐召燃;;電絮凝法處理偶氮染料廢水的脫色試驗(yàn)研究[J];印染助劑;2012年03期

7 徐雪松;許端平;褚海艷;;褐煤腐殖酸吸附脫除廢水中活性艷紅的研究[J];安全與環(huán)境學(xué)報(bào);2011年03期

8 董磊;喬俊蓮;閆麗;鄭廣宏;肖方;;微波協(xié)同活性炭處理偶氮染料廢水的研究[J];環(huán)境污染與防治;2010年04期

9 周覓;柳廣飛;周集體;金若菲;陳明翔;王艷青;;醌還原酶-醌類(lèi)化合物對(duì)偶氮染料脫色的作用[J];環(huán)境科學(xué);2009年06期

10 郭梅;路福平;劉敏堯;李拖平;梁鵬;蒲軍;;基因工程菌漆酶對(duì)蒽醌染料的脫色研究[J];環(huán)境科學(xué)與技術(shù);2009年04期

相關(guān)碩士學(xué)位論文 前1條

1 陳積義;微波輻射處理高濃度苯酚廢水及再生活性炭的試驗(yàn)研究[D];湖南大學(xué);2007年

，

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