甘薯響應(yīng)蔓割病菌侵染的轉(zhuǎn)錄組分析及抗病相關(guān)基因挖掘
發(fā)布時(shí)間:2022-01-28 01:09
甘薯是重要的糧食、飼料、工業(yè)原料作物,也是糧食安全的底線作物。甘薯的塊根及莖葉營養(yǎng)價(jià)值高,已成為公認(rèn)的健康食品。甘薯根系發(fā)達(dá),莖蔓有著地生根的習(xí)性,吸水吸肥能力強(qiáng),對(duì)土壤和日照長度要求不嚴(yán)格,適應(yīng)性廣,在世界各地均有種植。目前全世界甘薯栽培面積達(dá)1.2億畝,總產(chǎn)1億噸。作為世界第七大糧食作物,廣泛種植于世界上100多個(gè)國家,以亞洲種植面積最大,非洲排在第二位。中國甘薯栽培面積5千萬畝,總產(chǎn)7千多萬噸,分別占世界的42%和68%。自從16世紀(jì)傳入中國之后,甘薯便在福建和廣東一帶栽培并傳播到長江流域和黃河流域,形成了多個(gè)栽培區(qū)域。在非洲甘薯是繼玉米、水稻、小麥和木薯之后第五大糧食作物。隨著世界經(jīng)濟(jì)的發(fā)展,甘薯在各國的消費(fèi)形式也呈現(xiàn)多樣化,美國等發(fā)達(dá)國家對(duì)甘薯的利用主要表現(xiàn)在防癌保健食品開發(fā)和做為優(yōu)質(zhì)蔬菜食用,發(fā)展中國家作為糧食的利用沒有衰退,主要進(jìn)行淀粉加工、優(yōu)質(zhì)鮮薯食用、菜用市場開發(fā)、保健食品開發(fā)等。一些非洲國家將甘薯作為食物,甚至作為食物能量的主要來源。從紫色甘薯中提取花青素,用于食品加工和保健食品的制作也有著非常廣闊的前景。但是甘薯在生產(chǎn)上面臨著許多病蟲害的威脅,其中甘薯蔓割。ǹ菸...
【文章來源】:福建農(nóng)林大學(xué)福建省
【文章頁數(shù)】:96 頁
【學(xué)位級(jí)別】:碩士
【文章目錄】:
Appendix List of Abbrevation
中文摘要
Abstract
1. Introduction
1.1 Background
1.2 Sweet potato in China
1.3 Introduction to Fusarium oxysporum
1.4 Fusarium distribution
1.5 Disease cycle of fusarium
1.6 Fusarium disease process
1.7 Mycelium of fusrium
1.8 Mechanism of interaction between fungal and plant
1.9 Identification of Effector and R gene
1.10 Signal transduction in relation to plant-pathogen interaction
1.11 Transcription factor in plants and ungal
1.12 Transcriptome research
1.13 Control of fungal (F.oxysporum)
1.14 Objective of the study
1.15 Significance of study
2. Materials and Methods
2.1 Plant material, treatment and RNA extraction
2.2 Library preparation and Transcriptome sequencing
2.3 Quality control and de novo assembly
2.4 Gene functional annotation
2.5 Differential expression analysis
2.6 Experimental validation
2.7 Mining for SSR markers
3. Results
3.1 RNA sequencing and de novo transcriptome assembly
3.2 Functional annotation
3.3 GO and KOG classification
3.4 KEGG pathway mapping
3.5 Experimental validation by qRT-PCR
3.6 Gene expression profile of Sweet potato response to Fob
3.7 Identification of related genes involved in defense response
3.7.1 WRKY transcription factor
3.7.2 NAC transcription factors
3.7.3 Ethylene response transcription factor
3.7.4 MYB transcription factor
3.7.5 Pathogenesis-related (PR) protein
3.7.6 Resistance (R) genes
3.7.7 Genes associate with Sallicyllic acid (SA) pathway
3.8 Plant-pathogen interaction pathway
3.9 SSR determination
4. Discussion and Conclusion
4.1 discussion
4.2 conclusion
Reference
Acknowledgement
【參考文獻(xiàn)】:
期刊論文
[1]尖孢鐮刀菌與寄主互作機(jī)理研究進(jìn)展[J]. 裴月令,曾凡云,彭軍,龍海波,郭建榮. 熱帶生物學(xué)報(bào). 2014(01)
[2]中國甘薯病毒種類的血清學(xué)和分子檢測[J]. 喬奇,張振臣,張德勝,秦艷紅,田雨婷,王永江. 植物病理學(xué)報(bào). 2012(01)
[3]香蕉枯萎病菌侵染香蕉根系的組織學(xué)過程[J]. 殷曉敏,徐碧玉,鄭雯,曾會(huì)才,馬蔚紅,王家保,李煥苓,金志強(qiáng). 植物病理學(xué)報(bào). 2011(06)
[4]福建省甘薯蔓割病現(xiàn)狀與研究進(jìn)展[J]. 方樹民,陳玉森. 植物保護(hù). 2004(05)
[5]甘薯在我國糧食和能源安全中的重要作用[J]. 劉慶昌. 科技導(dǎo)報(bào). 2004(09)
本文編號(hào):3613411
【文章來源】:福建農(nóng)林大學(xué)福建省
【文章頁數(shù)】:96 頁
【學(xué)位級(jí)別】:碩士
【文章目錄】:
Appendix List of Abbrevation
中文摘要
Abstract
1. Introduction
1.1 Background
1.2 Sweet potato in China
1.3 Introduction to Fusarium oxysporum
1.4 Fusarium distribution
1.5 Disease cycle of fusarium
1.6 Fusarium disease process
1.7 Mycelium of fusrium
1.8 Mechanism of interaction between fungal and plant
1.9 Identification of Effector and R gene
1.10 Signal transduction in relation to plant-pathogen interaction
1.11 Transcription factor in plants and ungal
1.12 Transcriptome research
1.13 Control of fungal (F.oxysporum)
1.14 Objective of the study
1.15 Significance of study
2. Materials and Methods
2.1 Plant material, treatment and RNA extraction
2.2 Library preparation and Transcriptome sequencing
2.3 Quality control and de novo assembly
2.4 Gene functional annotation
2.5 Differential expression analysis
2.6 Experimental validation
2.7 Mining for SSR markers
3. Results
3.1 RNA sequencing and de novo transcriptome assembly
3.2 Functional annotation
3.3 GO and KOG classification
3.4 KEGG pathway mapping
3.5 Experimental validation by qRT-PCR
3.6 Gene expression profile of Sweet potato response to Fob
3.7 Identification of related genes involved in defense response
3.7.1 WRKY transcription factor
3.7.2 NAC transcription factors
3.7.3 Ethylene response transcription factor
3.7.4 MYB transcription factor
3.7.5 Pathogenesis-related (PR) protein
3.7.6 Resistance (R) genes
3.7.7 Genes associate with Sallicyllic acid (SA) pathway
3.8 Plant-pathogen interaction pathway
3.9 SSR determination
4. Discussion and Conclusion
4.1 discussion
4.2 conclusion
Reference
Acknowledgement
【參考文獻(xiàn)】:
期刊論文
[1]尖孢鐮刀菌與寄主互作機(jī)理研究進(jìn)展[J]. 裴月令,曾凡云,彭軍,龍海波,郭建榮. 熱帶生物學(xué)報(bào). 2014(01)
[2]中國甘薯病毒種類的血清學(xué)和分子檢測[J]. 喬奇,張振臣,張德勝,秦艷紅,田雨婷,王永江. 植物病理學(xué)報(bào). 2012(01)
[3]香蕉枯萎病菌侵染香蕉根系的組織學(xué)過程[J]. 殷曉敏,徐碧玉,鄭雯,曾會(huì)才,馬蔚紅,王家保,李煥苓,金志強(qiáng). 植物病理學(xué)報(bào). 2011(06)
[4]福建省甘薯蔓割病現(xiàn)狀與研究進(jìn)展[J]. 方樹民,陳玉森. 植物保護(hù). 2004(05)
[5]甘薯在我國糧食和能源安全中的重要作用[J]. 劉慶昌. 科技導(dǎo)報(bào). 2004(09)
本文編號(hào):3613411
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