本研究探討了我國(guó)西部森林中昆蟲病原真菌（EFs）的分布與致病力。本研究由兩部分組成:第一部分是確定秦嶺佛坪自然保護(hù)區(qū)（FNNR）內(nèi)昆蟲病原真菌;第二部分是四川森林巴山與川西森林中的昆蟲病原真菌。從佛坪自然保護(hù)區(qū)分離到綠僵菌,白僵菌等昆蟲病原真菌,使用核基因的DNA序列數(shù)據(jù)和形態(tài)學(xué)特征鑒定種類并分析系統(tǒng)發(fā)育關(guān)系。使用Mz_IGS3基因片段分析佛坪自然保護(hù)區(qū)EFs的進(jìn)化關(guān)系,通過(guò)PARB進(jìn)化樹對(duì)綠僵菌Metarhizium進(jìn)行分析可以明確鑒定到四個(gè)不同的種及其進(jìn)化關(guān)系;但是5’-TEF基因片段僅能夠?qū)G僵菌種類進(jìn)行有限而模糊的區(qū)分。白僵菌則是通過(guò)對(duì)翻譯延伸因子EF-1α和Bloc區(qū)域的序列分析來(lái)進(jìn)行鑒定和分析。綠僵菌物種的豐富度隨著海拔的升高而降低,其中綠僵菌（Metarhizium robertsii s.l.）是沿海拔梯度最豐富的物種。來(lái)自四川省的EFs的形態(tài)學(xué)和分子特征證實(shí)了昆蟲真菌病原體的四個(gè)主要屬,即綠僵菌Metarhizium（173株）,白僵菌Beauveria（15株）,棒束孢屬Isaria（10株）,淡紫紫霉屬Purpureocillium（1株）和一... 

【文章來(lái)源】：西北農(nóng)林科技大學(xué)陜西省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：119 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
ABSTRACT
摘要
附圖
CHAPTER ONE: INTRODUCTION
    1.1 General view
    1.2 Necessity of study and destructive impacts of biological pesticides
    1.3 Natural occurrence of the entomopathogenic fungi species in nature
    1.4 Cryptic species in fungal entomopathogens and the use of molecular delimitation forecological studies
    1.5 Are fungi relatively free from dispersal barriers?
    1.6 The applicability of the beneficial symbioant to fungal entomopathogens
    1.7 Entomopathogenic fungi as a biological control agent
    1.8 Thesis scope
CHAPTER TWO: GENERAL METHODS
    2.1 Study site and soil sampling
    2.2 Isolation of insect pathogenic fungi (Metarhizium and Beauveria) from FNNR soilsamples by an in vitro semi-differential medium method
    2.3 Fungal isolation from Sichuan province soil samples with insect susceptible bait method
    2.4 Morphology of 20 Metarhizium isolates from FNNR
    2.5 Genomic DNA extraction, PCR amplification and sequencing:
    2.6 Phylogenetic analysis:
    2.7 Bioassay experiment:
        2.7.1 Experimental insects
        2.7.2 Preparation of conidia
    2.8 Cold activity on conidial germination
    2.9 Deposit of fungal strain in public service collection
    2.10 Statistical analysis
    2.11 Mantel test and species richness
CHAPTER THREE: RESULTS
    3.1 RESULTS OF EFs FROM FNNR
        3.1.1 Metarhizium occurrence and DNA analysis
        3.1.2 Morphology of isolates and culture studies
        3.1.3 Growth assessment and in vitro conidiation study
        3.1.4 Virulence
        3.1.5 Pathogenicity against L. dispar
        3.1.6 Cold activity
    3.2 Results from Sichuan Province
        3.2.1 Occurrence and distribution of the entomopathogenic fungi
        3.2.2 Phylogenetic analysis of fungal isolates
CAHPTER FOUR: DISCUSSION AND CONCLUSION
    4.1 Discussion
    4.2 Conclusion
CHAPTER FIVE: SUPPLEMENTARY DATA
References
LIST OF FIGURES
LIST OF TABLES
List of Abbreviations
Aknowledgement
Personal introduction
List of Publications



本文編號(hào)：3304315