【摘要】：目的建立并比較凍干紅細(xì)胞殘余水含量測定方法,探討凍干紅細(xì)胞殘余水的分布情況。方法一次性凍干等量(500μL)的含保護(hù)劑(主要成分12%甘油)的紅細(xì)胞、無保護(hù)劑的紅細(xì)胞,含保護(hù)劑(同前)的血影細(xì)胞、無保護(hù)劑的血影細(xì)胞及保護(hù)劑,用熱重分析法及Karl-Fisher滴定法結(jié)合卡式爐法測定其殘余水含量,同時(shí)用含水量固定的標(biāo)準(zhǔn)品(水分含量4.9%-5.2%)做檢驗(yàn),每組樣品重復(fù)測量6次;分析各組之間的差異,比較凍干血影細(xì)胞及凍干紅細(xì)胞殘余水含量。結(jié)果熱重分析法測定殘余水含量(%):凍干紅細(xì)胞、無保護(hù)劑凍干紅細(xì)胞、凍干血影細(xì)胞、無保護(hù)劑凍干血影細(xì)胞、單純凍干保護(hù)劑、標(biāo)準(zhǔn)品分別為19.01±2.18、4.60±0.78、18.95±1.89、4.87±1.01、16.12±1.04、5.28±0.16;凍干保護(hù)劑及含保護(hù)劑凍干紅細(xì)胞的熱重(TG)曲線走勢接近,無保護(hù)劑凍干紅細(xì)胞與前二者曲線走勢差異明顯;Karl-Fisher法測定殘余水含量(%):凍干紅細(xì)胞、無保護(hù)劑凍干紅細(xì)胞、凍干血影細(xì)胞、無保護(hù)劑凍干血影細(xì)胞、單純凍干保護(hù)劑、標(biāo)準(zhǔn)品分別為3.21±0.23、1.22±0.09、3.16±0.26、1.25±0.07、2.63±0.41、5.14±0.13。結(jié)論熱重分析法測定含保護(hù)劑的凍干品殘余水含量時(shí)的結(jié)果偏高,Karl-Fisher滴定法結(jié)合卡式爐法能夠準(zhǔn)確反映凍干紅細(xì)胞的殘余水含量;初步判斷凍干紅細(xì)胞殘余水主要分布于細(xì)胞膜。
[Abstract]:Objective to establish and compare the method for the determination of residual water in freeze-dried red blood cells and to study the distribution of residual water in freeze-dried red blood cells. Methods RBC containing protective agent (12% glycerol), erythrocyte without protective agent, blood shadow cell containing protective agent (same as before), blood shadow cell without protective agent and protective agent were freeze-dried at one time (500 渭 L). The residual water content was determined by thermogravimetric analysis and Karl-Fisher titration combined with calcareous furnace method. At the same time, the content of residual water was tested with the standard sample with water content fixed (4.9- 5.2%), and the samples were measured six times repeatedly in each group. The difference between each group was analyzed and the residual water content of freeze-dried blood shadow cells and freeze-dried red blood cells was compared. Results the residual water content (%) was determined by thermogravimetric analysis: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protective agents. The standard samples were 19.01 鹵2.180.60 鹵0.78U 18.95 鹵1.89C 4.87 鹵1.01C 16.12 鹵1.04U 5.28 鹵0.16, respectively. The trend of thermogravimetric (TG) curve of freeze-dried erythrocytes was close to that of freeze-dried erythrocytes, but the trend of freeze-dried erythrocytes without protective agents was obviously different from that of the former. The content of residual water (%) was determined by Karl-Fisher: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protectants. The standard samples were 3.21 鹵0.23C 1.22 鹵0.09U 3.16 鹵0.26C 1.25 鹵0.072.63 鹵0.41g 5.14 鹵0.13, respectively. Conclusion the results of thermogravimetric analysis for the determination of residual water in freeze-dried products containing protective agents are high. Karl-Fisher titration combined with calcareous furnace method can accurately reflect the residual water content of freeze-dried red blood cells. It was preliminarily estimated that the residual water of freeze-dried red blood cells was mainly distributed in the cell membrane.
【作者單位】： 安徽醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院輸血研究所;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81370669)
【分類號(hào)】：R457.1

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