【摘要】：目的了解昌平區(qū)腸集聚性黏附大腸埃希氏菌(EAEC)耐藥性、毒力基因攜帶情況及分子特征。方法對2012—2016年北京市昌平區(qū)分離的15株EAEC毒株進行復核;以藥敏實驗檢測菌株對16種抗菌藥物的耐藥性;以PCR法檢測8種毒力的攜帶情況;采用多位點序列分型(MLST)及脈沖場凝膠電泳分型(PFGE)進行分子分型分析。結(jié)果 15株EAEC耐藥率為100.0%,耐多藥率為80.0%。每株攜帶2~5個毒力基因,共有10種毒力基因譜;8個毒力基因陽性率由高到低分別為aap(100.0%)、CVD432(93.3%)、aggA(60.0%)、agg3A(40.0%)、astA(33.3%)、pic(20.0%)、aafA(13.3%),未檢出pet陽性。MLST分型分析將15株EAEC菌分為9個ST型,其中有4個新ST型(STcpEC1、STcpEC2、STcpEC3、STcpEC4),以STcpEC1為優(yōu)勢ST型(占33.3%)。PFGE分析得到13種PFGE帶型,相同ST型菌株P(guān)FGE帶型、毒力基因相似系數(shù)均較高。結(jié)論北京市昌平區(qū)近5年EAEC菌耐藥率、多重耐藥率較高,毒力基因aap、CVD432、aggA檢出率高,STcpEC1為優(yōu)勢ST型,PFGE帶型具有高度多態(tài)性。
[Abstract]:Objective to investigate the drug resistance, virulence gene carrying and molecular characteristics of enteric agglutinating Escherichia coli (EAEC) in Changping area. Methods 15 strains of EAEC isolated from Changping District of Beijing from 2012 to 2016 were examined for their resistance to 16 antimicrobial agents by drug sensitivity test and 8 strains for carrying virulence by PCR. Multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) were used for molecular typing. Results the resistance rate of 15 strains of EAEC was 100.0% and the rate of multi-drug resistance was 80. 0%. Each strain carried 2 ~ 5 virulence genes, with 10 virulence gene profiles, 8 virulence gene positive rates from high to low were aap (100.0%), CVD432 (93. 3%), aap (60. 0%), astA (33. 3%) (33. 0%) and aafA (13. 3%). The 15 strains were classified into 9 St types by pet positive. Among them, there were four new St types (STcpEC1 / STcpEC2 / STcpEC3 / STcpEC4). Among them, 13 kinds of PFGE bands were obtained by using STcpEC1 as the dominant ST-type (33.3%). The similar coefficient of virulence genes of the same ST-type strains was higher. Conclusion the resistance rate and multidrug resistance rate of EAEC in Changping District of Beijing in recent 5 years are high. The detection rate of virulence gene aappEC1 is high and STcpEC1 is the dominant ST-type PFGE band.
【作者單位】： 北京市昌平區(qū)疾病預防控制中心;
【分類號】：R440

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