本文選題：激光熱毀損 + 離體豬肝��； 參考：《光譜學(xué)與光譜分析》2017年10期

【摘要】：提出了利用近紅外散射光譜對生物組織熱毀損程度進行實時鑒別的一種新方法。實驗首先分別用功率3.5,5,6.5和8 W的808nm脈沖激光對新鮮離體豬肝進行熱毀損,在距離毀損中心10mm處實時采集近紅外散射光譜和溫度,以最小二乘法擬合得到波長830~900nm處的光譜斜率(S830~900),將其不同上升幅度為條件結(jié)束加熱。其次,對光譜采集點附近的組織作病理切片,根據(jù)組織學(xué)特征進行評分,采用3級評分制(3分為完全毀損,2分為部分毀損,1分為無效毀損)評估毀損程度。最后,分析加熱期S830~900最大上升幅度、降溫后S830~900最終穩(wěn)定值與病理分析的毀損程度之間的關(guān)聯(lián)。結(jié)果表明,一般情況下,若加熱期S830~900上升至初值的4倍以上、降溫后最終穩(wěn)定于3.5倍以上,對應(yīng)組織為完全毀損;若S830~900加熱期上升2~5倍,降溫后最終穩(wěn)定于1~1.5倍,對應(yīng)組織為無效毀損;其余為部分毀損。根據(jù)分析結(jié)果,首次建立了光譜因子與熱毀損程度的關(guān)聯(lián),利用加熱期S830~900的最大上升幅度與降溫后的最終穩(wěn)定值來鑒別組織毀損程度,可以很好的降低實驗對象個體差異性對判斷結(jié)果的影響,具有很好的鑒別效果,其準確率達到90%以上,實現(xiàn)了組織熱毀損程度實時、準確的鑒別,為組織熱毀損實時監(jiān)控提供了一種新思路。
[Abstract]:A new method for real time identification of thermal damage of biological tissues by near infrared scattering spectroscopy is proposed.In this experiment, the fresh isolated porcine liver was damaged by 808nm pulse laser with power of 3.5 and 8 W, respectively. The near infrared scattering spectra and temperature were collected in real time at the 10mm distance from the damage center.The spectral slope of the wavelength 830~900nm was obtained by least square fitting, and the different rising amplitude was used as the condition to end the heating.Secondly, histopathological sections were made in the vicinity of the spectral sampling points. According to the histological characteristics, the degree of damage was evaluated by using a 3-grade scoring system, which was divided into complete damage and partial damage, divided into partial damage and partial damage, and divided into invalid damage.Finally, the relationship between the final stable value of S830T900 during heating period and the damage degree of pathological analysis was analyzed.The results show that, under normal conditions, if S830T900 rises to more than 4 times of initial value during heating period, it will eventually stabilize at 3.5 times after cooling, and the corresponding tissue will be destroyed completely, and if S830T900 increases 25 times during heating period, after cooling, it will eventually stabilize at 11. 5 times.The corresponding organization is invalid damage; the rest is partial damage.Based on the results of the analysis, the correlation between the spectral factor and the degree of thermal damage was established for the first time. The maximum rise of S830T900 during the heating period and the final stable value after cooling were used to identify the degree of tissue damage.It can reduce the influence of the individual difference on the result of the experiment, and has a good discriminant effect. The accuracy rate is over 90%, which realizes the real-time and accurate identification of the degree of tissue thermal damage.It provides a new idea for real-time monitoring of tissue thermal damage.
【作者單位】： 南通大學(xué)機械工程學(xué)院;南京航空航天大學(xué)生物醫(yī)學(xué)工程系;
【基金】：國家自然科學(xué)基金項目(61405098)資助
【分類號】：O657.3;R45
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本文編號：1759859