本文選題：感染　切入點：中樞神經(jīng)系統(tǒng)　出處：《中國感染與化療雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的探討聚合酶鏈反應(PCR)在細菌和真菌性中樞神經(jīng)系統(tǒng)感染快速診斷中的臨床價值。方法收集137例中樞神經(jīng)系統(tǒng)感染患者留存的腦脊液進行DNA提取,采用細菌和真菌通用引物擴增病原體DNA并進行序列測定,對比同期腦脊液培養(yǎng)方法與PCR方法的檢測結(jié)果。結(jié)果 137份腦脊液標本中PCR檢測到細菌50株,真菌6株,腦脊液培養(yǎng)檢測到細菌38株,真菌5株;PCR檢測法靈敏度為40.9%,特異度為100%,陽性預測值為100%,陰性預測值為38.2%,診斷效率為56.7%;傳統(tǒng)培養(yǎng)法則分別為31.4%、100%、100%、34.7%、44.4%。PCR的靈敏度、陰性預測值和診斷效率均明顯優(yōu)于傳統(tǒng)培養(yǎng)方法,特異度和陽性預測值與培養(yǎng)法相當,兩方法鑒定菌種的符合率為97.7%。結(jié)論通用引物PCR擴增法具有快速、特異、靈敏、準確等特點,對細菌和真菌性中樞神經(jīng)系統(tǒng)感染的病原學快速診斷具有重要的應用價值。
[Abstract]:Objective to investigate the clinical value of polymerase chain reaction (PCR) in the rapid diagnosis of bacterial and fungal central nervous system infections. The pathogen DNA was amplified by bacterial and fungal universal primers and sequenced. The results of CSF culture method and PCR method were compared. Results 50 strains of bacteria and 6 strains of fungi were detected by PCR in 137 samples of cerebrospinal fluid. The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic efficiency were 40.9, 100, 100, 38.2, 56.7, respectively, respectively. The negative predictive value and diagnostic efficiency were obviously superior to those of the traditional culture method. The specificity and positive predictive value of the two methods were similar to those of the culture method. The coincidence rate of the two methods for identifying the strains was 97.7.The conclusion the universal primer PCR amplification method is rapid, specific and sensitive. It has important application value for rapid etiological diagnosis of bacterial and fungal central nervous system infection.
【作者單位】： 首都醫(yī)科大學宣武醫(yī)院檢驗科;江西衛(wèi)生職業(yè)學院檢驗系;
【基金】：首都臨床特色重點專項課題(Z141107002514012)
【分類號】：R440;R742.9

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