本文關(guān)鍵詞： 外泌體 肺癌 磷脂酰肌醇蛋白聚糖　出處：《中國老年學雜志》2017年18期 　論文類型：期刊論文

【摘要】：目的觀察X線輻射對A549肺腺癌細胞周期及對細胞上清外泌體磷脂酰肌醇蛋白聚糖(GPC)1表達的影響。方法培養(yǎng)肺腺癌細胞A549并分別給予0、0.5、1、1.5、2 Gy劑量照射,照射后繼續(xù)培養(yǎng)6、12 h收集細胞,用PI法檢測細胞周期;收集細胞上清液,超速離心法提取外泌體,Western印跡檢測外泌體GPC1表達。結(jié)果 G_2期細胞的比例隨著劑量的增加而增加,差異有統(tǒng)計學意義(P0.001),其他各劑量組與2 Gy組比較差異有統(tǒng)計學意義(P0.05);各劑量組S期細胞比例均高于0 Gy組(P0.05);Western印跡結(jié)果顯示,隨輻射劑量增大,GPC1表達量逐漸降低。結(jié)論輻射能夠誘導A549細胞G2期、S期阻滯,同時細胞上清外泌體GPC1蛋白表達減少,二者具有相關(guān)性。
[Abstract]:Objective to observe the effects of X-ray radiation on the cell cycle and the expression of phosphatidylinositol proteoglycan 1 in the supernatant of A549 lung adenocarcinoma cells. The cell cycle was detected by Pi assay, the supernatant was collected and the exocrine GPC1 expression was detected by Western blotting with ultracentrifugation. The difference was statistically significant (P 0.001), and there was significant difference between other dose groups and 2 Gy groups (P 0.05), and the percentage of S phase cells in each dose group was higher than that in 0 Gy group (P 0.05), and the results of Western blotting showed that the percentage of S phase cells in each dose group was higher than that in 0 Gy group. Conclusion radiation can induce G 2 phase arrest of A549 cells and decrease the expression of GPC1 protein in the exocrine of the cell supernatant. There is a correlation between the expression of GPC1 and the expression of GPC1 protein in A549 cells.
【作者單位】： 長春市人民醫(yī)院;吉林省申邦細胞工程研究所;吉林大學第二醫(yī)院;
【基金】：吉林省科技發(fā)展計劃項目(20160101104JC)
【分類號】：R730.55
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本文編號：1542823