本文關鍵詞：四路熒光采集系統(tǒng)　出處：《山東師范大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 高速數(shù)據(jù)采集 激光誘導熒光 多通道

【摘要】：本課題針對基于微流控芯片中的多組分熒光同時檢測要求,目前針對激光誘導熒光微流控芯片的儀器多針對單組份測量,無法實現(xiàn)多組分的快速同時測量。本課題就是基于這一現(xiàn)實狀況下進行研究設計的。由于用不同波長的激光照射染色后的細胞發(fā)出的熒光比較微弱,因此如何對細胞熒光這一微弱信號進行檢測是這套儀器能否準確工作的關鍵。這是由生物化學研究作為理論依據(jù)的,利用電子工程技術中的電路設計和軟件編程支持其完成實際的應用功能,所以這是一個多學科相互融合的工程項目。本設計是利用光電倍增管(PMT)對光信號進行采集,光電倍增管將采集到的熒光數(shù)據(jù)經(jīng)過I/V轉(zhuǎn)換送到信號調(diào)理板,經(jīng)過對熒光信號的濾波和放大后,送至數(shù)據(jù)采集卡,數(shù)據(jù)采集卡將采集到的信號傳至上位機,上位機將采集到的數(shù)據(jù)以Excel表格的形式存儲到電腦硬盤,將數(shù)據(jù)進行處理之后就會得到細胞的數(shù)量和細胞內(nèi)成分等信息。本文章主要設計了一套高采集速率,高精度,多分組同時采集的一體化采集設備,這套系統(tǒng)實現(xiàn)了對細胞熒光信號采集以及自動化處理。本文章主要分為了六個部分,具體如下:第一,文章的緒論部分主要包括四路熒光采集系統(tǒng)的課題來源,研究課題的背景及意義,國內(nèi)外的發(fā)展現(xiàn)狀。第二,系統(tǒng)的總體設計,講述了對系統(tǒng)設計方案的可行性研究,介紹了系統(tǒng)的基本功能及元件選型的分析。第三,這部分介紹了系統(tǒng)的詳細設計方案,包括系統(tǒng)電源的設計,PMT控制模塊的設計,信號調(diào)理模塊的設計,AD數(shù)據(jù)采集模塊的設計,主程序軟件的設計,PCB電路板的設計與制作。第四,上位機的設計,包括了對LabView系統(tǒng)開發(fā)平臺的簡單介紹,還包括了主控制界面軟件的設計。第五,主要介紹了對儀器的使用方法和對細胞熒光數(shù)據(jù)的采集結(jié)果。最后,本章對現(xiàn)在完成的工作做了總結(jié),然后對后續(xù)系統(tǒng)的進一步改進做了展望。
[Abstract]:This paper aims at the requirement of multicomponent fluorescence detection based on microfluidic chip. At present, the instruments for laser-induced fluorescence microfluidic chip are mostly aimed at single component measurement. It is impossible to realize the rapid simultaneous measurement of multicomponent. This subject is based on this reality. Because of the different wavelength of laser irradiation the fluorescence emitted by the cells is relatively weak. Therefore, how to detect the weak signal of cell fluorescence is the key to the accuracy of the instrument, which is based on biochemical research. Using the circuit design and software programming in the electronic engineering technology to support it to complete the actual application function. So this is a multidisciplinary fusion of engineering projects. This design is to use photomultiplier tube (PMT) to collect optical signals. The photomultiplier tube converts the collected fluorescence data to the signal conditioning board through I / V conversion. After filtering and amplifying the fluorescence signal, it is sent to the data acquisition card, which transmits the collected signal to the upper computer. The data collected by the host computer is stored in the form of Excel form to the hard disk of the computer. After processing the data, we will get the information of cell number and intracellular components. This paper mainly designed a set of integrated acquisition equipment with high acquisition rate, high precision and multi-group collection at the same time. This system has realized the acquisition of cell fluorescence signal and automatic processing. This article is mainly divided into six parts, the details are as follows: first, the introduction of the article mainly includes four sources of fluorescence acquisition system. The background and significance of the research, the development of the status quo at home and abroad. Second, the overall design of the system, described the feasibility of the system design scheme, introduced the basic functions of the system and component selection analysis. Third. This part introduces the detailed design of the system, including the system power supply design PMT control module design, signal conditioning module design and AD data acquisition module design, the main program software design. The design and manufacture of PCB circuit board. 4th, the design of host computer, including the simple introduction of LabView system development platform, and the design of main control interface software. 5th. This paper mainly introduces the use of the instrument and the collection results of the cell fluorescence data. Finally, this chapter summarizes the work done now, and then the further improvement of the follow-up system is prospected.
【學位授予單位】：山東師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TP274.2

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