本文關(guān)鍵詞：大豆生物活性肽對(duì)仔豬生長(zhǎng)性能的影響及其機(jī)理的研究，由筆耕文化傳播整理發(fā)布。

        本研究是以大豆生物活性肽為原料，著重研究了大豆肽對(duì)仔豬的生產(chǎn)性能、腹瀉發(fā)生率、血清學(xué)指標(biāo)的影響，并且從免疫學(xué)、腸道組織形態(tài)學(xué)和腸道微生物組成三個(gè)方面對(duì)大豆肽的影響機(jī)制進(jìn)行了初步探討。 一、大豆生物活性肽對(duì)斷奶仔豬生產(chǎn)性能的影響 試驗(yàn)共選取初始體重相近的35±2日齡斷奶仔豬240頭(杜×長(zhǎng)×大)，隨機(jī)分為5個(gè)處理，每個(gè)處理6個(gè)重復(fù)，每個(gè)重復(fù)8頭豬(各重復(fù)公母比例相同)。分別飼喂空白日糧、含75mg／kg金霉素對(duì)照日糧、1000mg／kg、3000mg／kg及5000mg／kg大豆生物活性肽的試驗(yàn)日糧。飼養(yǎng)期為25天，記錄每個(gè)處理的腹瀉情況，計(jì)算日增重、平均日采食量，計(jì)算飼料轉(zhuǎn)化效率。第25天時(shí)從頸靜脈采血，用以測(cè)定血清蛋白質(zhì)、血糖、血清尿素氮等生化指標(biāo)。試驗(yàn)表明，大豆生物活性肽添加到仔豬日糧中后，可以顯著提高仔豬的飼料轉(zhuǎn)化效率(P＜0.05)，同時(shí)降低腹瀉率(P＜0.05)。由本試驗(yàn)得出的大豆生物活性肽在斷奶仔豬料中的適宜添加量為3000mg／kg。大豆生物活性肽可以提高仔豬血清中的總蛋白(P＜0.01)、球蛋白(P＜0.01)的含量。以3000mg／kg濃度添加時(shí)可以提高血糖(P＜0.01)和血清尿素氮含量(P＜0.05)。 二、大豆生物活性肽對(duì)育成初期豬只生產(chǎn)性能的影響 選取健康、體格接近的70±3日齡的豬只192頭，按體重和性別隨機(jī)分配到三個(gè)處理中，每個(gè)處理4個(gè)重復(fù)，每個(gè)重復(fù)16頭豬，分別飼喂空白日糧、3000mg／kg大豆肽及75mg／kg金霉素，飼養(yǎng)期為30天。結(jié)果表明，在育成初期階段，抗生素與肽的添加對(duì)日增重、飼料轉(zhuǎn)化效率已不再產(chǎn)生顯著的促進(jìn)作用(P＞0.05)，但是抗生素和肽仍然表現(xiàn)出一定的促生長(zhǎng)趨勢(shì)，其中3000mg／kg肽組與空白組相比，料重比降低了約18.2％，效果優(yōu)于抗生素，故仍然可以考慮添加，這樣有利于提高肉質(zhì)，減少藥物帶來(lái)的不良影響。 三、大豆生物活性肽促進(jìn)仔豬生產(chǎn)性能機(jī)理的研究 本試驗(yàn)分別從免疫學(xué)、腸道組織形態(tài)學(xué)以及微生物學(xué)三個(gè)方面探討了大豆活性肽的作用機(jī)理，試驗(yàn)使用的是與試驗(yàn)一相同日齡的豬只，在試驗(yàn)期結(jié)束時(shí)(25天)，進(jìn)行采血和屠宰，用以測(cè)定抗體水平、腸絨毛高度、隱窩深度及腸道微生物組成。試驗(yàn)結(jié)果表明，添加大豆生物活性肽的試驗(yàn)組，其對(duì)牛血清白蛋白產(chǎn)生的抗體水平有依濃度增加而提高的趨勢(shì)，并且均高于兩個(gè)對(duì)照組，接近顯著性水平(P=0.0501)。3000mg／kg肽水平可以提高部分腸段的絨毛高度，并加深部分隱窩深度，，但顯著性不一。同時(shí)，大豆生物活性肽有一定的抑菌效果(主要針對(duì)耗氧型細(xì)菌)，其抑制沙門氏菌的效果在結(jié)腸已經(jīng)顯著高于抗生素(P＜0.01)，而對(duì)乳酸菌，在結(jié)腸則顯現(xiàn)出了極顯著的促進(jìn)作用(P＜0.01)，但同時(shí)又顯著抑制了乳酸菌在盲腸的數(shù)量(P＜0.01)。

    This research centered on the effects of soybean peptides on growth performance of weanling piglets. Furthermore, the mechanism of these effects was investigated on aspects of immunology, intestinal histology and gut micro-flora.Experiment 1: Effects of soybean oligopeptides (SBP) on growth performance of weanling pigs240 35-days-old cross-bred (DoricXLandraceXLarge White) weaned piglets with initial weight at 8.78 + 0.98kg were randomly allotted to five treatments with six replicates per treatment and eight pigs per pen (four gilts and four barrows) at similar initial body weight. Basal diet was fed to the control group and basal diet+75mg/kg Aureomycin, 1000mg/kg SBP, 3000mg/kg SBP, 5000mg/kg SBP supplement to other experimental groups, respectively. Results showed that addition of 3000mg/kg SBP significantly increased ADG and decreased feed/gain efficiency (P<0.05) while diarrhea incidences were significantly decreased by the addition of 1000mg/kg SBP (P<0.05) . As for ADFI, the differences among all groups were not significant. Experiment 2: Effects of SBP on growth performance of young growing pigs192 70-days-old cross-bred (DoricXLandraceXLarge White) young growing pigs with initial weight at 16.68 +0.67kg were randomly allotted to three treatments with four replicates per treatment and sixteen pigs per pen (four gilts and four barrows) at similar initial body weight. Basal diet was fed to the control group and basal diet +75mg/kg Aureomycin, 3000mg/kg SBP supplement to other experimental groups, respectively. Results showed that addition of 3000mg/kg SBP increased ADG and decreased feed/gain efficiency by a certain amount which was better than the other two groups on a non-significant level (P>0.05). Experiment 3: Mechanism of the growth-promoting effects on weanling pigs of SBPThe design of this experiment was the same as that of experiment 1, and aimed at discovering the mechanism of the effects showed in the former two experiments by means of immunology, intestinal histology and gut microbiology. The pigs were inoculated by BSA twice to ensure the animals could produce high levels of antibodies in the blood. Four pigs from each of the control group, Aureomycin group and 3000mg/kg SBP group were slaughtered after 25 days of experiment. The results showed that SBP added up to the level of blood antibodies and might affect increasingly or decreasingly to different stains in cecum and colon. The main inhibiting effects appeared on aerobes (E. coli and salmonella), while lactobacilli were promoted in the colon. At the same time, intestinal mucosal villi were heightened and crypts were deepened significantly in the SBP groups, and the percentage of goblet cell was increased ultra significantly as well.

大豆生物活性肽對(duì)仔豬生長(zhǎng)性能的影響及其機(jī)理的研究

第一章 緒論8-22    1 肽營(yíng)養(yǎng)理論的提出與發(fā)展的簡(jiǎn)要?dú)v程8-10    2 肽營(yíng)養(yǎng)的研究與應(yīng)用10-17    3 活性肽生物制品17-21    4 本研究的內(nèi)容和意義21-22第二章 大豆肽對(duì)斷奶仔豬生產(chǎn)性能影響的研究22-31    1 試驗(yàn)材料與方法22-23    2 試驗(yàn)結(jié)果與分析23-28    3 討論28-30    4 小結(jié)30-31第三章 大豆肽對(duì)育成初期豬只生長(zhǎng)性能影響的研究31-34    1 試驗(yàn)材料與方法31-32    2 試驗(yàn)結(jié)果與分析32-33    3 討論33    4 小結(jié)33-34第四章 大豆生物活性肽提高仔豬生產(chǎn)性能機(jī)理的研究34-51    1 大豆生物活性肽對(duì)仔豬抗體水平的影響34-35    2 大豆生物活性肽對(duì)仔豬腸道組織形態(tài)學(xué)的影響35-43    3 大豆生物活性肽對(duì)仔豬結(jié)腸、盲腸微生物組成的影響43-46    4 討論46-50    5 小結(jié)50-51第五章 本試驗(yàn)主要結(jié)論及有待進(jìn)一步研究的問(wèn)題51-52參考文獻(xiàn)52-61附錄61-68致謝68-69作者簡(jiǎn)歷69

  本文關(guān)鍵詞：大豆生物活性肽對(duì)仔豬生長(zhǎng)性能的影響及其機(jī)理的研究，由筆耕文化傳播整理發(fā)布。