本文選題：壓載水 + 微藻　； 參考：《大連海事大學(xué)》2017年碩士論文

【摘要】：隨著我國航運(yùn)事業(yè)的發(fā)展,船舶壓載水中微生物跨區(qū)域傳播的危害問題也越來越嚴(yán)重,因此對(duì)海洋中的微藻進(jìn)行成像與檢測具有重要的意義。傳統(tǒng)的成像及檢測設(shè)備存在體積大、價(jià)格昂貴、需要較高的操作水平、無法進(jìn)行現(xiàn)場檢測等缺點(diǎn)。為了實(shí)現(xiàn)壓載水中微藻的成像與檢測,本文設(shè)計(jì)了一種同軸無透鏡數(shù)字全息微顆粒成像系統(tǒng)。該系統(tǒng)實(shí)現(xiàn)了微顆粒的成像,獲得了微顆粒的三維形貌及三維尺寸,并通過對(duì)海水中扁藻的成像證明了該系統(tǒng)可用于海洋微藻的成像及活性檢測。本文的主要工作有:(1)從標(biāo)量衍射理論出發(fā)分析了同軸無透鏡數(shù)字全息微顆粒成像系統(tǒng)中光源波長、小孔直徑、光源到樣品的距離以及樣品的記錄距離等參數(shù),推導(dǎo)了各參數(shù)之間的關(guān)系。在設(shè)計(jì)同軸無透鏡數(shù)字全息成像系統(tǒng)時(shí),可按照它們之間的關(guān)系得出成像系統(tǒng)各部分的最佳搭配。(2)設(shè)計(jì)了同軸無透鏡數(shù)字全息微顆粒成像系統(tǒng)。該系統(tǒng)利用部分相干光源LED作為全息干涉的光源,通過耦合小孔增加部分相干光源的相干性,以蓋玻片為平臺(tái),利用去除透鏡的CMOS圖像傳感器獲得了微顆粒的同軸全息圖。(3)對(duì)比分析了菲涅爾積分變換、菲涅爾卷積及角譜重建算法,得到了不同重建算法的適用范圍,并推導(dǎo)了利用角譜重建算法時(shí)的再現(xiàn)距離的計(jì)算公式。(4)利用共軛像消除算法獲得了消除了共軛實(shí)像的同軸全息再現(xiàn)像,并利用解包裹算法獲得了微顆粒的真實(shí)相位,重建了微顆粒的三維形貌并獲得了微顆粒三維尺寸。(5)拍攝了海水中扁藻的全息圖,重建了扁藻的再現(xiàn)像,消除了扁藻再現(xiàn)像的共軛像,并恢復(fù)了扁藻的三維形貌。最后將扁藻的相位信息與顯微鏡下的扁藻圖像作對(duì)比,對(duì)扁藻的活性進(jìn)行了判斷,證明了該系統(tǒng)不僅可以獲得微藻的三維信息,也可以用于海洋中微藻活性的檢測。
[Abstract]:With the development of shipping industry in China, the problem of transregional spread of microorganism in ship ballast water is becoming more and more serious, so it is of great significance to image and detect microalgae in the ocean. The traditional imaging and detection equipment has the disadvantages of large volume, high price, high level of operation, unable to carry out field inspection and so on. In order to realize the imaging and detection of microalgae in ballast water, a coaxial lensless digital holographic microparticle imaging system is designed in this paper. The system has realized the imaging of microparticles, obtained the three-dimensional morphology and size of microparticles, and proved that the system can be used for the imaging and activity detection of marine microalgae by imaging the algae in seawater. Based on the scalar diffraction theory, the parameters such as the wavelength of the light source, the diameter of the aperture, the distance from the light source to the sample and the recording distance of the sample in the digital holographic microparticle imaging system with coaxial lens are analyzed. The relationship between the parameters is deduced. In the design of the coaxial lensless digital holographic imaging system, according to the relationship between them, the optimum collocation of each part of the imaging system can be obtained. (2) the coaxial lensless digital holographic microparticle imaging system is designed. In this system, the partially coherent light source (LED) is used as the light source for holographic interference, and the coherence of the partially coherent light source is increased through the coupling holes, and the cover glass is used as the platform. The coaxial holograms of microparticles are obtained by using the CMOS image sensor which removes the lens. The Fresnel integral transform, Fresnel convolution and angular spectrum reconstruction algorithms are compared and analyzed, and the applicable range of the different reconstruction algorithms is obtained. The formula for calculating the reconstruction distance of the angular spectrum reconstruction algorithm is derived. The conjugate image elimination algorithm is used to obtain the coaxial holographic reconstruction image which eliminates the conjugate real image, and the real phase of the microparticle is obtained by unwrapping algorithm. The three-dimensional morphology of the microparticle was reconstructed and the three-dimensional size of the microparticle was obtained. The hologram of the alga in seawater was taken, the reconstructed image of the alga was reconstructed, the conjugate image of the reconstructed image of the alga was eliminated, and the three-dimensional morphology of the alga was restored. Finally, the phase information of the alga is compared with the microalgae image under the microscope, and the activity of the alga is judged. It is proved that the system can not only obtain the three-dimensional information of the microalgae, but also can be used to detect the activity of the microalgae in the ocean.
【學(xué)位授予單位】：大連海事大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TP391.41

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本文編號(hào)：1812903