鎘(Cd²⁺)是一種劇毒非必需元素,對(duì)全球農(nóng)作物生產(chǎn)和人類健康構(gòu)成嚴(yán)重威脅。為了研究外源脫落酸（ABA）是否可以緩解Cd²⁺脅迫并且提高Cd²⁺敏感型生菜（Lactuca sativa L）品種的食品安全性,我們開(kāi)展了以下試驗(yàn):1)第一個(gè)試驗(yàn)比較了十個(gè)生菜品種（意大利,歐歌,四季,綠夢(mèng),碧羽衣,美國(guó),隴盛,美惠子,綠速和安研）的Cd²⁺積累能力和食品安全潛力;2)在第二個(gè)試驗(yàn)中,確定了篩選的四種生菜品種（綠速,綠夢(mèng),意大利和安研）對(duì)Cd²⁺脅迫的根系耐受性和生化響應(yīng);3)在第三個(gè)試驗(yàn)中,研究了葉面施用ABA對(duì)Cd²⁺敏感型生菜（綠速）生理生化、與ABA,赤霉素（GA3）,吲哚-3-乙酸（IAA）,細(xì)胞分裂素（CTK）合成相關(guān)的基因相對(duì)表達(dá)以及生物量和葉片鎘含量的影響。所有試驗(yàn)均在溫室育苗,然后轉(zhuǎn)移至水培營(yíng)養(yǎng)液中在氣候控制室中進(jìn)行處理。試驗(yàn)結(jié)果表明:1)0.1μM CdCl₂九個(gè)生菜品種的葉片干生物量降低了4.1-12.6%。然而,綠夢(mèng)... 

【文章來(lái)源】：甘肅農(nóng)業(yè)大學(xué)甘肅省

【文章頁(yè)數(shù)】：114 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
SUMMARY
摘要
CHAPTER ONE 1.0. Literature Review
    1.1. Plant abiotic stress and effect of heavy metals on plants
    1.2. Cadmium uptake and its transport in plants
    1.3. Effect of cadmium on plants
    1.4. Bio-indicators of the level of stress in plants
    1.5. Plants tolerance mechanisms to heavy metal stress
        1.5.1. Antioxidant defense system in plants
        1.5.2. Role of endogenous hormones
        1.5.3. ABA synthesis and alleviation of heavy metal stress in plants
        1.5.4. Expression of genes in plants under heavy metal stress
    1.6. Strategies for managing cadmium stress in plants
        1.6.1 Use of tolerant cultivars
        1.6.2. Application of exogenous plant growth regulators
        1.6.3. ABA biosynthesis and its application in managing heavy metal stress
        1.6.4. Use of grafted materials
        1.6.5. Plant nutrition and alleviation of Cd stress in plants
        1.6.6. Use of organic soil amendments in alleviating Cd stress in plants
    1.7. Problem statement
    1.8. Justification/Significance of the study
    1.9. Research hypotheses
    1.10. Objectives of the study
CHAPTER TWO 2.0. Cadmium bioaccumulation and safety potentials of ten Chinese lettuce (Lactuca sativa L.)genotypes grown in Cd~(2+)-contaminated hydroponic nutrient solution
    2.1. Introduction
    2.2 Materials and Methods
        2.2.1. Genotypes used for the experiment
        2.2.2. Seed germination and seedling growth
        2.2.3. Treatments and experimental design
        2.2.4. Measurement of growth indexes
        2.2.5. Analysis of cadmium contents
        2.2.6. Statistical analysis
    2.3. Results
        2.3.1 Main effects of genotype and cadmium on growth indexes
        2.3.2 Effect of variety and cadmium on chlorophyll (SPAD value), fresh and dry leaf biomass and contents of Cd in the plants
        2.3.4. Correlation analysis for vegetative characteristics and cadmium contents
    2.4. Discussion
    2.5 Conclusion
CHAPTER THREE 3.0. Root tolerance and biochemical response of Chinese lettuce (Lactuca sativa L.) Genotypes to cadmium stress
    3.1. Introduction
    3.2. Materials and Methods
        3.2.1. Genotypes used for the experiment
        3.2.2. Seedling growth conditions
        3.2.3. Experimental design and treatments
        3.2.4. Data measurements
        3.2.5. Statistical analysis
    3.3. Results
        3.3.1. Root morphological characteristics
        3.3.2. Root tolerance to cadmium stress
        3.3.3. Contents of H_2O_2 in roots
        3.3.4. Contents of root hormones
        3.3.5. Correlation analysis
        3.3.6. Antioxidant enzymes activities and proline contents
    3.4. Discussion
    3.5 Conclusion
CHAPTER FOUR 4.0. Foliar application of Abscisic acid mitigates cadmium stress and increases food safety of cadmium-sensitive lettuce (Lactuca sativa L.) genotype
    4.1. Introduction
    4.2. Materials and Methods
        4.2.1. Plant materials and growth conditions
        4.2.2. Experimental design and treatments
        4.2.3. Data measurements
    4.3. Results
        4.3.1. Hydrogen peroxide (H_2O_2) and malondialdehyde (MDA) contents
        4.3.2. Antioxidant enzymes activities
        4.3.3. Chlorophyll (Chl) and carotenoids contents
        4.3.4. Gas exchange parameters
        4.3.5. Relative water content (RWC) of leaf
        4.3.6. Expressions of genes related to signal transduction of ABA, GA3, IAA and CTK
        4.3.7. Macro and micro nutrient elements
        4.3.8. Cadmium contents
        4.3.9. Biomass, biomass response to stress (BRS) and morphology of lettuce plants
    4.4. Discussion
    4.5 Conclusion
CHAPTER FIVE
    5.0. General conclusions
    5.1. Innovation
    5.2. Recommendations
REFERENCES
APPENDICES
ACKNOWLEDGEMENTS
DEDICATION
BRIFE INTRODUCTION OF AUTHOR
BRIFE INTRODUCTION OF SUPERVISOR


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期刊論文
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[2]Combined toxicity of copper and cadmium to six rice genotypes （Oryza sativa L.）[J]. HUANG Yizong, HU Ying, LIU Yunxia Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China..  Journal of Environmental Sciences. 2009(05)
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