達(dá)爾文氏棉耐鹽基因資源的挖掘與分析
發(fā)布時間：2021-12-24 21:59
鹽脅迫是影響棉花幼苗生長、產(chǎn)量和品質(zhì)的重要因素。長期人工選擇和馴化,導(dǎo)致陸地棉品種的遺傳基礎(chǔ)狹窄,因此更多的科學(xué)家試投通過遺傳重組將野生棉種的優(yōu)異基因?qū)朐耘嗥贩N中,以拓寬栽培棉種的遺傳基礎(chǔ)。本研究旨在挖掘和分析四倍體野生種達(dá)爾文氏棉耐鹽的基因源。實(shí)驗材料是達(dá)爾文氏棉07（AD507）,利用陸地棉品種中棉所12（CCRI12）為參照材料。在研究果糖1,6-二磷酸醛縮酶（FBA）基因家族的功能和表達(dá)模式時,利用陸地棉（TM-1）、亞洲棉（石系亞1號）和雷蒙德氏棉（雷蒙德氏棉07）等3個棉種的全基因組測序數(shù)據(jù),分析得到37個FBA基因（其中陸地棉19個,亞洲棉和雷蒙德氏棉各9個）。根據(jù)蛋白質(zhì)結(jié)構(gòu)域和系統(tǒng)發(fā)育分析,將FBA基因分為I和II兩類�；蚪Y(jié)構(gòu)和基序的分析表明,多數(shù)FBA基因的內(nèi)含子分布不均勻。據(jù)該基因家族的共線性區(qū)域分析,發(fā)現(xiàn)31個重復(fù)基因,分為3種類型:片段型、串聯(lián)型和分散型。片段型占多數(shù)FBA基因,較為保守,這可能是FBA基因在棉花中擴(kuò)增的原因。GO分析結(jié)果表明,FBA基因參與了液泡分隔、糖酵解和鋅離子結(jié)合等一系列生物學(xué)過程。在AD5...
【文章來源】： 中國農(nóng)業(yè)科學(xué)院北京市
【文章頁數(shù)】：131 頁
【文章目錄】：
附件
摘要
abstract
Abbreviations
CHAPTER1.INTRODUCTION
1.1 WILD COTTON AND GOSSYPIUM DARWINII
1.1.1 Importance of Cotton
1.1.2 Cotton Taxonomy,Wild Cotton and Gossypium Darwinii
1.2 SALINITY,ITS EFFECTS ON PLANTS AND PANT TOLERENCES TO SALINITY
1.2.1 Types of Salinity
1.2.2 Factors Effect Plant Tolerance to Salinity
1.2.3 Plant Responses to Salinity
1.2.4 Three Major Tolerance Mechanisms of Plants to Salinity
1.3 IMPORTANCE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE（FBA）
1.4 SNPS AND ITS ADVANTAGES TO OTHER MOLECULAR MARKERS
1.5 BULKED SEGREGATION ANALYSIS（BSA）
1.5.1 BSA and Its Current Status
1.5.2 Bulks,Sampling and Bulking
1.5.3 Analyses:DNA,RNA and Protein
1.6 RESEARCH OBJECTIVES
CHAPTER2.MATERIALS AND METHODS
2.1 PLANT MATERIALS AND DATA RESOURCES
2.1.1 The De Novo Sequenced Data
2.1.2 G.darwinii with its Referenced G.hirsutum,Their BC2F2 Population and Genetic Linkage Map
2.2 METHODS
2.2.1 Identification,Localization and Structure of FBA Genes
2.2.2 Phylogenetic Analysis
2.2.3 Chromosomal Location and Syntenic Analysis
2.2.4 Gene Ontology（GO）Annotation
2.2.5 Salt Treatment
2.2.6 RNA Extraction
2.2.7 RNA Sequence
2.2.8 Salt Tolerance Identification and RT-qPCR Validation
2.2.9 Gene Mining within SSR Markers,Prediction of mi RNA Target and Cis Elements Analysis
2.2.10 Construction of Sample Pools in the BC2F2 Population
2.2.11 Library Constuction and Resequencing
2.2.12 Assemly and Calculaton of SNP-index
CHAPTER3.RESULTS
3.1 GENOME WIDE ANALYSIS ON FBA GENE FAMILY AND RT-QPCR VALIDATION FOR SALT TOLERENCES
3.1.1 Phylogenetic Analysis and FBA Gene Identifications in the Three De Novo Sequenced Cotton Species
3.1.2 GO Annotation for FBA Gene Functions
3.1.3 RT-q PCR Validation of the Candidate Genes for Salt Telorence in G.darwinii with G.hirsutum as Reference
3.2 IDENTIFICATION AND VALIDATION OF PKINASE GENES FOR SALT TOLERANCES
3.2.1 Pkinase Gene Identification Analysis in A Genetic Map of G.darwinii with G.hirsutum
3.2.2 Structure and Location the Pkinase Genes
3.2.3 GO Annotation for Pkinase Gene Functions
3.2.4 Duplication and Syntenic Analysis of Pkinase Genes in in Three De Novo Sequenced Cotton Species
3.2.5 Prediction of Transcription Factor Biding Sites and mi RNA Analysis of the Pkinase Genes
3.2.6 RNA Sequencing and RT-q PCR Validation of the Pkinase Genes for Salt Tolerance in G.darwinii with G.hirsutum as Reference
3.3.BULKED SEGREGANT ANALYSIS（BSA）ON BC2F2 POPULATION OF G.DARWINII WITH G.HIRSUTUM UNDER SALT STRESS
3.3.1 Phenotypic Identification under Salt Stress and Construction of BSA Pools
3.3.2 Resequencing and Candidate Genes Prediction
3.3.3 Validation of the Candidate Genes in G.darwinii with G.hirsutum as Reference
CHAPTER4.DISCUSSION
4.1 GENIC POTENTIAL OF SALT TOLERANCES IN G.DARWINII
4.2 BSA ANALYSIS
4.3 CHARACTERIZATION OF FBA GENE FAMILY UNDER SALT STRESS
4.4 PHYLOGENY,EXON-INTRON STRUCTURES AND EXPANSION OF FBA GENES IN COTTON
4.5 FUNCTIONAL ANALYSIS OF FBA GENES
4.6 PROTEIN KINASE GENE FAMILY IMPACTS SALINITY STRESS TOLERANCE IN HIGHLY DENSE GENETIC MAP
4.7 ADVANTAGES OF BC2F2 POPULATION AND BSA MARKER
CHAPTER5.CONCLUSIONS
5.1 GENOME WIDE CHARACTERIZATION OF FBA GENE FAMILY UNDER SALT STRESS IN GOSSYPIUM SPECIES
5.2 GENOME-WIDE MINING AND IDENTIfiCATION OF PROTEIN KINASE GENE FAMILY IMPACTS SALINITY STRESS TOLERANCE IN HIGHLY DENSE GENETIC MAP
5.3 BSA MAPPING OF BC2F2 POPULATION
CURRICULUM VITAE
REFERENCES
APPENDICES
【參考文獻(xiàn)】：
期刊論文
[1]棉花耐鹽性的SSR標(biāo)記研究 [J]. 張麗娜,葉武威,王俊娟,樊保香. 棉花學(xué)報. 2010(02)
本文編號：3551269
【文章來源】： 中國農(nóng)業(yè)科學(xué)院北京市
【文章頁數(shù)】：131 頁
【文章目錄】：
附件
摘要
abstract
Abbreviations
CHAPTER1.INTRODUCTION
1.1 WILD COTTON AND GOSSYPIUM DARWINII
1.1.1 Importance of Cotton
1.1.2 Cotton Taxonomy,Wild Cotton and Gossypium Darwinii
1.2 SALINITY,ITS EFFECTS ON PLANTS AND PANT TOLERENCES TO SALINITY
1.2.1 Types of Salinity
1.2.2 Factors Effect Plant Tolerance to Salinity
1.2.3 Plant Responses to Salinity
1.2.4 Three Major Tolerance Mechanisms of Plants to Salinity
1.3 IMPORTANCE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE（FBA）
1.4 SNPS AND ITS ADVANTAGES TO OTHER MOLECULAR MARKERS
1.5 BULKED SEGREGATION ANALYSIS（BSA）
1.5.1 BSA and Its Current Status
1.5.2 Bulks,Sampling and Bulking
1.5.3 Analyses:DNA,RNA and Protein
1.6 RESEARCH OBJECTIVES
CHAPTER2.MATERIALS AND METHODS
2.1 PLANT MATERIALS AND DATA RESOURCES
2.1.1 The De Novo Sequenced Data
2.1.2 G.darwinii with its Referenced G.hirsutum,Their BC2F2 Population and Genetic Linkage Map
2.2 METHODS
2.2.1 Identification,Localization and Structure of FBA Genes
2.2.2 Phylogenetic Analysis
2.2.3 Chromosomal Location and Syntenic Analysis
2.2.4 Gene Ontology（GO）Annotation
2.2.5 Salt Treatment
2.2.6 RNA Extraction
2.2.7 RNA Sequence
2.2.8 Salt Tolerance Identification and RT-qPCR Validation
2.2.9 Gene Mining within SSR Markers,Prediction of mi RNA Target and Cis Elements Analysis
2.2.10 Construction of Sample Pools in the BC2F2 Population
2.2.11 Library Constuction and Resequencing
2.2.12 Assemly and Calculaton of SNP-index
CHAPTER3.RESULTS
3.1 GENOME WIDE ANALYSIS ON FBA GENE FAMILY AND RT-QPCR VALIDATION FOR SALT TOLERENCES
3.1.1 Phylogenetic Analysis and FBA Gene Identifications in the Three De Novo Sequenced Cotton Species
3.1.2 GO Annotation for FBA Gene Functions
3.1.3 RT-q PCR Validation of the Candidate Genes for Salt Telorence in G.darwinii with G.hirsutum as Reference
3.2 IDENTIFICATION AND VALIDATION OF PKINASE GENES FOR SALT TOLERANCES
3.2.1 Pkinase Gene Identification Analysis in A Genetic Map of G.darwinii with G.hirsutum
3.2.2 Structure and Location the Pkinase Genes
3.2.3 GO Annotation for Pkinase Gene Functions
3.2.4 Duplication and Syntenic Analysis of Pkinase Genes in in Three De Novo Sequenced Cotton Species
3.2.5 Prediction of Transcription Factor Biding Sites and mi RNA Analysis of the Pkinase Genes
3.2.6 RNA Sequencing and RT-q PCR Validation of the Pkinase Genes for Salt Tolerance in G.darwinii with G.hirsutum as Reference
3.3.BULKED SEGREGANT ANALYSIS（BSA）ON BC2F2 POPULATION OF G.DARWINII WITH G.HIRSUTUM UNDER SALT STRESS
3.3.1 Phenotypic Identification under Salt Stress and Construction of BSA Pools
3.3.2 Resequencing and Candidate Genes Prediction
3.3.3 Validation of the Candidate Genes in G.darwinii with G.hirsutum as Reference
CHAPTER4.DISCUSSION
4.1 GENIC POTENTIAL OF SALT TOLERANCES IN G.DARWINII
4.2 BSA ANALYSIS
4.3 CHARACTERIZATION OF FBA GENE FAMILY UNDER SALT STRESS
4.4 PHYLOGENY,EXON-INTRON STRUCTURES AND EXPANSION OF FBA GENES IN COTTON
4.5 FUNCTIONAL ANALYSIS OF FBA GENES
4.6 PROTEIN KINASE GENE FAMILY IMPACTS SALINITY STRESS TOLERANCE IN HIGHLY DENSE GENETIC MAP
4.7 ADVANTAGES OF BC2F2 POPULATION AND BSA MARKER
CHAPTER5.CONCLUSIONS
5.1 GENOME WIDE CHARACTERIZATION OF FBA GENE FAMILY UNDER SALT STRESS IN GOSSYPIUM SPECIES
5.2 GENOME-WIDE MINING AND IDENTIfiCATION OF PROTEIN KINASE GENE FAMILY IMPACTS SALINITY STRESS TOLERANCE IN HIGHLY DENSE GENETIC MAP
5.3 BSA MAPPING OF BC2F2 POPULATION
CURRICULUM VITAE
REFERENCES
APPENDICES
【參考文獻(xiàn)】：
期刊論文
[1]棉花耐鹽性的SSR標(biāo)記研究 [J]. 張麗娜,葉武威,王俊娟,樊保香. 棉花學(xué)報. 2010(02)
本文編號：3551269
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