蘇云金芽胞桿菌MEP代謝途徑相關(guān)基因的轉(zhuǎn)錄調(diào)控研究
發(fā)布時間：2021-01-12 17:44
蘇云金芽胞桿菌（Bacillus thuringensis,Bt）,是一種革蘭氏陽性細菌,在生成芽胞的同時還產(chǎn)生對多種農(nóng)林害蟲有殺蟲活性的伴胞晶體,是應(yīng)用最廣的微生物殺蟲劑。萜類化合物廣泛分布在生物界,是重要的生命物質(zhì)。目前發(fā)現(xiàn)有兩條萜類化合物的生物合成途徑,即甲羥戊酸（MVA）途徑和2-甲基-D-赤蘚糖醇-4-磷酸（MEP）途徑。Bt中有8個基因編碼MEP代謝途徑相關(guān)的酶,本文對這些基因的轉(zhuǎn)錄調(diào)控進行研究。主要取得了如下結(jié)果:生物信息學(xué)分析發(fā)現(xiàn),Bt中有8個基因編碼MEP代謝途徑相關(guān)的酶,它們是:dxs基因（HD734479）編碼1-脫氧-D-木酮糖-5-磷酸合酶,dxr基因（HD733605/dxr1和HD734104/dxr2）編碼1-脫氧-D-木酮糖-5-磷酸還原異構(gòu)酶,ispD（HD730085）基因編碼2-C-甲基-D-赤蘚醇-4-磷酸胞苷轉(zhuǎn)移酶,ispE（HD730043）基因編碼4-胞啶-2-C-甲基-D-赤蘚醇激酶,ispF（HD7300...
【文章來源】：山西師范大學(xué)山西省
【文章頁數(shù)】：93 頁
【學(xué)位級別】：碩士
【部分圖文】：
比較蘇云金芽胞桿菌HD73與枯草芽孢桿菌168菌株MEP途徑Fig.3-1ComparisonofMEPpathwaybetweeninBtHD73andBs168stain對兩個菌株的MEP代謝途徑相關(guān)基因進行ProteinBlast比對（表3-1）
結(jié)果與分析31表3-1蘇云金芽胞桿菌HD73菌株MEP代謝途徑生物信息學(xué)分析Table3-1BioinformaticsanalysisofMEPpathwayinBtHD73strainGeneIDGenenameBs168IdentityGeneannotationsHD73_4479dxsBSU2427077.8%1-deoxy-D-xylulose-5-phosphatesynthaseHD73_3605dxrBSU1655056.5%1-deoxy-D-xylulose-5-phosphatereductoisomeraseHD73_4104dxrBSU1655070.1%1-deoxy-D-xylulose5-phosphatereductoisomeraseHD73_0085ispDBSU0090053.4%2-C-methyl-D-erythritol4-phosphatecytidylyltransferaseHD73_0043ipk/ispEBSU0046074.0%4-diphosphocytidyl-2-C-methyl-D-erythritolkinaseHD73_0086ispFBSU0091076.4%2-C-methyl-D-erythritol2,4-cyclodiphosphatesynthaseHD73_4584ispGBSU2507089.0%4-hydroxy-3-methylbut-2-en-1-yldiphosphatesynthaseHD73_4593ispHBSU2516074.1%4-hydroxy-3-methylbut-2-enyldiphosphatereductaseHD73_1728fniBSU2287060.2%isopentenyl-diphosphatedelta-isomeraseHD73_4480ispABSU2428059.5%farnesyldiphosphatesynthaseHD73_1741hepSBSU2276040.0%heptaprenyldiphosphatesynthasecomponentIHD73_1743hepTBSU2274063.8%heptaprenyldiphosphatesynthasecomponentIIHD73_4106uppSBSU1653070.5%undecaprenylpyrophosphatesynthase圖3-2dxs與ispA基因在BtHD73和Bs168中分布比較Fig.3-2ComparisonofdxsandispAgenebetweeninBtHD73andBs168stain
山西師范大學(xué)碩士學(xué)位論文32圖3-3dxr基因在BtHD73和Bs168中分布比較Fig.3-3ComparisonoftwodxrgenesbetweeninBtHD73andBs168stain圖3-4ispD與ispF基因在BtHD73和Bs168中分布比較Fig.3-4ComparisonofispDandispFgenebetweeninBtHD73andBs168stain圖3-5ispE基因在BtHD73和Bs168中分布比較Fig.3-5ComparisonofispEgenebetweeninBtHD73andBs168stain圖3-6ispG基因在BtHD73和Bs168中分布比較Fig.3-6ComparisonofispGgenebetweeninBtHD73andBs168stain
本文編號：2973245
【文章來源】：山西師范大學(xué)山西省
【文章頁數(shù)】：93 頁
【學(xué)位級別】：碩士
【部分圖文】：
比較蘇云金芽胞桿菌HD73與枯草芽孢桿菌168菌株MEP途徑Fig.3-1ComparisonofMEPpathwaybetweeninBtHD73andBs168stain對兩個菌株的MEP代謝途徑相關(guān)基因進行ProteinBlast比對（表3-1）
結(jié)果與分析31表3-1蘇云金芽胞桿菌HD73菌株MEP代謝途徑生物信息學(xué)分析Table3-1BioinformaticsanalysisofMEPpathwayinBtHD73strainGeneIDGenenameBs168IdentityGeneannotationsHD73_4479dxsBSU2427077.8%1-deoxy-D-xylulose-5-phosphatesynthaseHD73_3605dxrBSU1655056.5%1-deoxy-D-xylulose-5-phosphatereductoisomeraseHD73_4104dxrBSU1655070.1%1-deoxy-D-xylulose5-phosphatereductoisomeraseHD73_0085ispDBSU0090053.4%2-C-methyl-D-erythritol4-phosphatecytidylyltransferaseHD73_0043ipk/ispEBSU0046074.0%4-diphosphocytidyl-2-C-methyl-D-erythritolkinaseHD73_0086ispFBSU0091076.4%2-C-methyl-D-erythritol2,4-cyclodiphosphatesynthaseHD73_4584ispGBSU2507089.0%4-hydroxy-3-methylbut-2-en-1-yldiphosphatesynthaseHD73_4593ispHBSU2516074.1%4-hydroxy-3-methylbut-2-enyldiphosphatereductaseHD73_1728fniBSU2287060.2%isopentenyl-diphosphatedelta-isomeraseHD73_4480ispABSU2428059.5%farnesyldiphosphatesynthaseHD73_1741hepSBSU2276040.0%heptaprenyldiphosphatesynthasecomponentIHD73_1743hepTBSU2274063.8%heptaprenyldiphosphatesynthasecomponentIIHD73_4106uppSBSU1653070.5%undecaprenylpyrophosphatesynthase圖3-2dxs與ispA基因在BtHD73和Bs168中分布比較Fig.3-2ComparisonofdxsandispAgenebetweeninBtHD73andBs168stain
山西師范大學(xué)碩士學(xué)位論文32圖3-3dxr基因在BtHD73和Bs168中分布比較Fig.3-3ComparisonoftwodxrgenesbetweeninBtHD73andBs168stain圖3-4ispD與ispF基因在BtHD73和Bs168中分布比較Fig.3-4ComparisonofispDandispFgenebetweeninBtHD73andBs168stain圖3-5ispE基因在BtHD73和Bs168中分布比較Fig.3-5ComparisonofispEgenebetweeninBtHD73andBs168stain圖3-6ispG基因在BtHD73和Bs168中分布比較Fig.3-6ComparisonofispGgenebetweeninBtHD73andBs168stain
本文編號：2973245
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