【摘要】：植物光合作用是人類賴以生存的基礎(chǔ),葉綠體是植物進行光合作用的重要場所,葉色可作為葉綠體發(fā)育狀態(tài)的標記,葉色突變體是研究光合作用機制的優(yōu)良系統(tǒng)。通過EMS誘變篩選得到一個擬南芥葉色突變體F03-06,主要表型為葉片黃綠、葉脈深綠色、生長緩慢、株型矮小。遺傳分析表明,F03-06突變表型受隱性單核基因控制。利用圖位克隆技術(shù),通過粗定位和精細定位發(fā)現(xiàn)該突變體表型與擬南芥At5g54810基因的突變體表型相似。對At5g54810基因的測序結(jié)果顯示,其+865堿基由鳥嘌呤突變?yōu)橄汆堰?從而使編碼的丙氨酸變?yōu)樘K氨酸。At5g54810基因被報道編碼擬南芥色氨酸合成酶的β亞基,為進一步研究擬南芥色氨酸合成途徑與葉綠體發(fā)育之間的調(diào)控機制提供了新的遺傳材料。
[Abstract]:Plant photosynthesis is the basis of human survival, chloroplast is an important place for plant photosynthesis, leaf color can be used as a marker of chloroplast development state, leaf color mutant is an excellent system to study the mechanism of photosynthesis. A leaf color mutant F03 / 06 of Arabidopsis thaliana was screened by EMS mutagenesis. The main phenotypes were yellow and green leaves, dark green veins, slow growth and short plant type. Genetic analysis showed that the phenotype of F03 / 06 mutation was controlled by recessed mononuclear gene. The mutant phenotype of Arabidopsis thaliana At5g54810 gene was similar to the mutant phenotype of Arabidopsis thaliana gene by rough localization and fine localization by map cloning technique. The sequencing results of At5g54810 gene showed that the 865 base changed from guanine to adenine, which changed the encoded alanine into threonine. At5g54810 gene was reported to encode the 尾 subunit of Arabidopsis tryptophan Synthase, which provided a new genetic material for further study on the regulatory mechanism between Arabidopsis tryptophan synthesis pathway and chloroplast development.
【作者單位】： 旱區(qū)逆境生物學國家重點實驗室/西北農(nóng)林科技大學生命學院;
【基金】：國家自然科學基金項目(31400216) 中央高�；究蒲匈M項目(2014YB036;Z109021537)
【分類號】：Q943.2
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本文編號：2522630