【摘要】：根據(jù)鹽穗木鹽脅迫下響應(yīng)的轉(zhuǎn)錄組測(cè)序結(jié)果,參考鹽穗木HcRev1、HcRev3基因的ESTs序列設(shè)計(jì)熒光定量PCR特異性引物,建立檢測(cè)鹽穗木Revs基因相對(duì)表達(dá)量的熒光定量PCR方法,分析Rev1和Rev3基因在鹽穗木不同濃度鹽脅迫處理不同時(shí)間的轉(zhuǎn)錄水平。結(jié)果表明,HcRev1、HcRev3基因具有相似的表達(dá)模式,在100mmol·L~(-1)NaCl低鹽脅迫下表達(dá)穩(wěn)定,在300、500、700 mmol·L~(-1)NaCl脅迫下,隨脅迫濃度增高、脅迫時(shí)間延長(zhǎng),表達(dá)量升高。其中HcRev1在700 mmol·L~(-1)NaCl脅迫14 d后達(dá)到峰值,是對(duì)照組的4.63倍。HcRev3基因在300mmol·L~(-1)NaCl脅迫14 d時(shí),表達(dá)量迅速升高,是對(duì)照組的15.55倍,表達(dá)差異極顯著。研究結(jié)果說(shuō)明HcRev1、HcRev3基因都受鹽脅迫誘導(dǎo)表達(dá),提示HcRev1、HcRev3基因雖然表達(dá)量存在差異,但在鹽脅迫過(guò)程中參與了DNA損傷修復(fù)。研究有助于闡明Rev1、Rev3基因在DNA損傷修復(fù)和植物耐鹽性間的調(diào)控功能作用。
[Abstract]:According to the sequence analysis of transcription set of response to salt stress of salt panicle wood, the fluorescent quantitative PCR specific primers were designed according to the ESTs sequence of HcRev1,HcRev3 gene in salt panicle wood, and a fluorescence quantitative PCR method was established to detect the relative expression of Revs gene in salt panicle wood. The transcription levels of Rev1 and Rev3 genes in different concentrations of salt stress were analyzed. The results showed that the expression pattern of HcRev1,HcRev3 gene was similar, and it was stable under low salt stress of 100mmol 路L ~ (- 1) NaCl. Under the stress of 300500700 mmol 路L ~ (- 1) NaCl, the expression level increased with the increase of stress concentration and the prolongation of stress time. The expression of HcRev1 reached its peak at 14 days after NaCl stress, which was 4.63 times higher than that of the control group. The expression of HcRev3 gene increased rapidly at 14 days of 300mmol 路L-1 NaCl stress, which was 15.55 times higher than that of the control group, and the difference was very significant. The results showed that the expression of HcRev1,HcRev3 gene was induced by salt stress, suggesting that although the expression of HcRev1,HcRev3 gene was different, it was involved in the repair of DNA damage during salt stress. The study may help to elucidate the regulatory function of Rev1,Rev3 gene between DNA damage repair and salt tolerance in plants.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院;新疆生物資源基因工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：新疆重點(diǎn)實(shí)驗(yàn)室專(zhuān)項(xiàng)資金資助項(xiàng)目(2014KL001)~~
【分類(lèi)號(hào)】：Q943.2

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