【摘要】：根據(jù)鹽穗木鹽脅迫下響應的轉錄組測序結果,參考鹽穗木HcRev1、HcRev3基因的ESTs序列設計熒光定量PCR特異性引物,建立檢測鹽穗木Revs基因相對表達量的熒光定量PCR方法,分析Rev1和Rev3基因在鹽穗木不同濃度鹽脅迫處理不同時間的轉錄水平。結果表明,HcRev1、HcRev3基因具有相似的表達模式,在100mmol·L~(-1)NaCl低鹽脅迫下表達穩(wěn)定,在300、500、700 mmol·L~(-1)NaCl脅迫下,隨脅迫濃度增高、脅迫時間延長,表達量升高。其中HcRev1在700 mmol·L~(-1)NaCl脅迫14 d后達到峰值,是對照組的4.63倍。HcRev3基因在300mmol·L~(-1)NaCl脅迫14 d時,表達量迅速升高,是對照組的15.55倍,表達差異極顯著。研究結果說明HcRev1、HcRev3基因都受鹽脅迫誘導表達,提示HcRev1、HcRev3基因雖然表達量存在差異,但在鹽脅迫過程中參與了DNA損傷修復。研究有助于闡明Rev1、Rev3基因在DNA損傷修復和植物耐鹽性間的調控功能作用。
[Abstract]:According to the sequence analysis of transcription set of response to salt stress of salt panicle wood, the fluorescent quantitative PCR specific primers were designed according to the ESTs sequence of HcRev1,HcRev3 gene in salt panicle wood, and a fluorescence quantitative PCR method was established to detect the relative expression of Revs gene in salt panicle wood. The transcription levels of Rev1 and Rev3 genes in different concentrations of salt stress were analyzed. The results showed that the expression pattern of HcRev1,HcRev3 gene was similar, and it was stable under low salt stress of 100mmol 路L ~ (- 1) NaCl. Under the stress of 300500700 mmol 路L ~ (- 1) NaCl, the expression level increased with the increase of stress concentration and the prolongation of stress time. The expression of HcRev1 reached its peak at 14 days after NaCl stress, which was 4.63 times higher than that of the control group. The expression of HcRev3 gene increased rapidly at 14 days of 300mmol 路L-1 NaCl stress, which was 15.55 times higher than that of the control group, and the difference was very significant. The results showed that the expression of HcRev1,HcRev3 gene was induced by salt stress, suggesting that although the expression of HcRev1,HcRev3 gene was different, it was involved in the repair of DNA damage during salt stress. The study may help to elucidate the regulatory function of Rev1,Rev3 gene between DNA damage repair and salt tolerance in plants.
【作者單位】： 新疆大學生命科學與技術學院;新疆生物資源基因工程重點實驗室;
【基金】：新疆重點實驗室專項資金資助項目(2014KL001)~~
【分類號】：Q943.2

【相似文獻】

相關期刊論文 前5條

1 張霞;張富春;;鹽生植物鹽穗木懸浮細胞系的建立[J];新疆農業(yè)科學;2013年09期

2 周蓮潔;楊中敏;張富春;王艷;;新疆鹽穗木GRAS轉錄因子基因克隆及表達分析[J];西北植物學報;2013年06期

3 彭丹;張霞;張富春;;鹽穗木過氧化氫酶基因的克隆與功能分析[J];西北植物學報;2013年10期

4 張霞;常丹;彭丹;張富春;;極端耐鹽植物鹽穗木脫水脅迫響應基因Rd22的克隆及表達分析[J];生物技術;2013年06期

5 鮑乾;徐濤;張富春;;鹽穗木miRNA417的克隆及對種子萌發(fā)和幼苗成活率的影響[J];植物研究;2011年04期

相關碩士學位論文 前2條

1 古麗內爾·亞森;高鹽脅迫下鹽穗木根的miRNA文庫的構建及miR398和miR395的表達分析[D];新疆大學;2014年

2 哈斯亞提汗·阿布都拉（Hasiyathan Abdulla）;鹽穗木金屬硫蛋白基因HcMT的克隆及對擬南芥的遺傳轉化[D];新疆大學;2014年

，

本文編號：2472474