發(fā)布時間：2019-05-05 13:45

【摘要】：應(yīng)用SSR分子標(biāo)記技術(shù),研究了以不同抗性轉(zhuǎn)基因741楊為食物的楊扇舟蛾實(shí)驗(yàn)種群的遺傳分化,探討以轉(zhuǎn)Bt基因楊樹作為食物對靶標(biāo)昆蟲的脅迫效應(yīng).利用篩選出的10對引物,分別對經(jīng)取食轉(zhuǎn)基因741楊高抗品系‘Pb29’、中抗品系‘Pb17’及未轉(zhuǎn)基因楊(對照)篩選出的楊扇舟蛾實(shí)驗(yàn)種群進(jìn)行遺傳多樣性和遺傳分化研究.結(jié)果表明:10對引物共檢測到76條等位基因,平均等位基因7.6個,平均有效等位基因?yàn)?.2,平均觀測雜合度為0.5167,平均期望雜合度為0.5167,平均多態(tài)位點(diǎn)百分率達(dá)96.7%.經(jīng)取食轉(zhuǎn)基因741楊篩選出的楊扇舟蛾實(shí)驗(yàn)種群的遺傳多樣性水平顯著高于未轉(zhuǎn)基因種群,且取食轉(zhuǎn)基因741楊的楊扇舟蛾與CK樣本間的遺傳相似性最低,表明經(jīng)取食轉(zhuǎn)Bt基因楊樹的楊扇舟蛾實(shí)驗(yàn)種群遺傳多樣性有增高趨勢.基于SSR分析,轉(zhuǎn)基因741楊對楊扇舟蛾實(shí)驗(yàn)種群的脅迫篩選效應(yīng)明顯.
[Abstract]:The genetic differentiation of the experimental population of Populus fan-boat moth with different resistance to transgenic poplar 741 was studied by using SSR molecular marker technique, and the stress effect of transgenic poplar with Bt gene on target insects was studied. The genetic diversity and genetic differentiation of the experimental population of Populus fan-boat moth selected by feeding on transgenic poplar high-resistance strain 'Pb29',' Pb17' and non-transgenic poplar (control) were studied by using 10 pairs of primers selected. The results showed that 76 alleles were detected in 10 pairs of primers, with an average of 7.6 alleles, an average effective allele of 2.2, an average observed heterozygosity of 0.5167 and an average expected heterozygosity of 0.5167. The average percentage of polymorphic loci was 96.7%. The genetic diversity level of the experimental population was significantly higher than that of the non-transgenic population, and the genetic similarity between the CK sample and the transgenic poplar was the lowest, and the genetic diversity level of the experimental population was significantly higher than that of the non-transgenic population, and the genetic similarity was the lowest between the experimental population and the non-transgenic population. The results showed that the genetic diversity of the experimental population of Bt-transgenic poplar had an increasing trend. Based on SSR analysis, transgenic poplar 741 had obvious effect on screening the experimental population.
【作者單位】： 河北農(nóng)業(yè)大學(xué)林學(xué)院;河北省林木種質(zhì)資源與森林保護(hù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家高技術(shù)研究發(fā)展計劃項(xiàng)目(2013AA102703) 國家林業(yè)局科技發(fā)展中心項(xiàng)目(JC-2014-04)資助~~
【分類號】：S763.7

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