【摘要】：根據(jù)雷公藤懸浮細(xì)胞轉(zhuǎn)錄組數(shù)據(jù)設(shè)計(jì)引物,克隆得到雷公藤鯊烯合酶基因Tw SQS全長(zhǎng)c DNA(Gen Bank:KR401220),并對(duì)其進(jìn)行生物信息學(xué)分析和誘導(dǎo)表達(dá)分析。TwSQS cDNA全長(zhǎng)為1 800 bp,開(kāi)放閱讀框?yàn)? 242 bp,編碼413個(gè)氨基酸,編碼的蛋白理論等電點(diǎn)為7.94,相對(duì)分子質(zhì)量為47.20 kD。雷公藤懸浮細(xì)胞經(jīng)茉莉酸甲酯(MJ)誘導(dǎo)后,熒光定量PCR表明TwSQS相對(duì)表達(dá)量明顯上升,并于誘導(dǎo)后4 h達(dá)到最高。本研究首次克隆得到雷公藤SQS基因,為進(jìn)一步解析雷公藤三萜類成分生物合成途徑奠定基礎(chǔ)。
[Abstract]:The full length cDNA (Gen Bank:KR401220 of squalene synthase gene of Tripterygium wilfordii (TwSQS) was cloned according to the data of suspension cell transcript group of Tripterygium wilfordii L. (Tripterygium wilfordii L.). Bioinformatics analysis and induced expression analysis were performed. The full length of Tws cDNA was 1 800 bp, An open reading frame (ORF) of 1 242 bp, encodes 413 amino acids, a theoretical isoelectric point of 7.94 and a relative molecular weight of 47.20 kD.. After the suspension cells were induced by methyl jasmonate (MJ), fluorescence quantitative PCR showed that the relative expression of TwSQS increased significantly, and reached the highest level at 4 h after induction. In this study, the SQS gene of Tripterygium wilfordii was cloned for the first time, which laid a foundation for further analysis of the biosynthesis pathway of triterpenoids.
【作者單位】： 首都醫(yī)科大學(xué)中醫(yī)藥學(xué)院;中國(guó)中醫(yī)科學(xué)院中藥資源中心;
【基金】：國(guó)家自然科學(xué)優(yōu)秀青年基金項(xiàng)目(81422053) 國(guó)家杰出青年基金項(xiàng)目(81325023)
【分類號(hào)】：S567.19;Q943.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 杜瑋煒;黃宏文;;雷公藤次生代謝產(chǎn)物雷公藤紅素含量與環(huán)境因子相關(guān)性分析[J];植物學(xué)通報(bào);2008年06期

2 陳喬爾,汪發(fā)貴,陳志武,戴俐明;雷公藤微囊長(zhǎng)期毒性的實(shí)驗(yàn)病理研究[J];安徽醫(yī)科大學(xué)學(xué)報(bào);1998年03期

3 鮑一笑,張玲珍,李莉,張登海,孔憲濤;Fas可能是雷公藤紅素誘導(dǎo)HMC-1細(xì)胞凋亡的途徑之一[J];中國(guó)免疫學(xué)雜志;1999年02期

4 ;[J];;年期

，

本文編號(hào)：2466078