【摘要】：根據(jù)雷公藤懸浮細(xì)胞轉(zhuǎn)錄組數(shù)據(jù)設(shè)計引物,克隆得到雷公藤鯊烯合酶基因Tw SQS全長c DNA(Gen Bank:KR401220),并對其進(jìn)行生物信息學(xué)分析和誘導(dǎo)表達(dá)分析。TwSQS cDNA全長為1 800 bp,開放閱讀框為1 242 bp,編碼413個氨基酸,編碼的蛋白理論等電點為7.94,相對分子質(zhì)量為47.20 kD。雷公藤懸浮細(xì)胞經(jīng)茉莉酸甲酯(MJ)誘導(dǎo)后,熒光定量PCR表明TwSQS相對表達(dá)量明顯上升,并于誘導(dǎo)后4 h達(dá)到最高。本研究首次克隆得到雷公藤SQS基因,為進(jìn)一步解析雷公藤三萜類成分生物合成途徑奠定基礎(chǔ)。
[Abstract]:The full length cDNA (Gen Bank:KR401220 of squalene synthase gene of Tripterygium wilfordii (TwSQS) was cloned according to the data of suspension cell transcript group of Tripterygium wilfordii L. (Tripterygium wilfordii L.). Bioinformatics analysis and induced expression analysis were performed. The full length of Tws cDNA was 1 800 bp, An open reading frame (ORF) of 1 242 bp, encodes 413 amino acids, a theoretical isoelectric point of 7.94 and a relative molecular weight of 47.20 kD.. After the suspension cells were induced by methyl jasmonate (MJ), fluorescence quantitative PCR showed that the relative expression of TwSQS increased significantly, and reached the highest level at 4 h after induction. In this study, the SQS gene of Tripterygium wilfordii was cloned for the first time, which laid a foundation for further analysis of the biosynthesis pathway of triterpenoids.
【作者單位】： 首都醫(yī)科大學(xué)中醫(yī)藥學(xué)院;中國中醫(yī)科學(xué)院中藥資源中心;
【基金】：國家自然科學(xué)優(yōu)秀青年基金項目(81422053) 國家杰出青年基金項目(81325023)
【分類號】：S567.19;Q943.2

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