【摘要】：豬α-(1,2)巖藻糖轉(zhuǎn)移酶2(FUT2)基因為鞘糖脂生物合成—球系列通路中的重要基因,可能在斷奶仔豬抵抗大腸桿菌F18侵染過程中發(fā)揮著調(diào)控作用。為探究豬FUT2基因的序列結(jié)構(gòu)及其生物學(xué)功能,試驗采用PCR擴(kuò)增得到地方豬品種東串豬FUT2基因的CDS全序列,進(jìn)而預(yù)測和分析FUT2基因的蛋白質(zhì)序列及其功能區(qū)域,同時對其在8頭35日齡東串?dāng)嗄套胸i11個組織中的表達(dá)水平進(jìn)行檢測與分析。結(jié)果顯示,FUT2基因的CDS序列全長為1 023bp,共編碼340個氨基酸,FUT2蛋白為脂溶性的親水蛋白,蛋白結(jié)構(gòu)不穩(wěn)定,該蛋白存在1個跨膜螺旋結(jié)構(gòu),但不存在信號肽,表明FUT2蛋白為膜蛋白;FUT2蛋白存在2個N-糖基化位點(185和305位氨基酸),無O-糖基化位點,此外該蛋白還存在14個潛在的磷酸化位點,包括6個Ser、2個Thr和6個Tyr,對其功能區(qū)域進(jìn)行分析發(fā)現(xiàn),FUT2蛋白存在1個超級家族保守結(jié)構(gòu)域:FUT1-FUT2-like(58-319位氨基酸);系統(tǒng)進(jìn)化樹結(jié)果顯示,豬與牛的親緣關(guān)系相對較近,與人、黑猩猩、大鼠和小鼠等親緣關(guān)系相對較遠(yuǎn);FUT2基因在東串?dāng)嗄套胸i11個組織中均有表達(dá),在消化道和免疫組織中表達(dá)水平較高。試驗結(jié)果推測FUT2基因在斷奶仔豬抵抗大腸桿菌F18中可能具有一定的作用,且可能是通過合成巖藻糖轉(zhuǎn)移酶間接發(fā)揮其抵抗大腸桿菌F18的作用。
[Abstract]:Porcine 偽-(1, 2) fucosyltransferase 2 (FUT2) gene may play a regulatory role in the resistance of weaned piglets to E. coli F18 infection because of the important genes in the glycosyl lipid biosynthesis-globular pathway. In order to investigate the sequence structure and biological function of porcine FUT2 gene, the full CDS sequence of FUT2 gene was amplified by PCR, and the protein sequence and functional region of FUT2 gene were predicted and analyzed. At the same time, its expression level in 11 tissues of 8 35-day-old weanling piglets was detected and analyzed. The results showed that the full-length CDS sequence of FUT2 gene was 1 023 BP, encoding a total of 340 amino acids. The FUT2 protein was a lipid soluble hydrophilic protein, and the structure of the protein was unstable. There was a cross-membrane helix structure in the protein, but no signal peptide. The results showed that FUT2 protein was a membrane protein. There were 2 N-glycosylation sites (185,305 amino acids) and no O-glycosylation sites in FUT2 protein. In addition, there were 14 potential phosphorylation sites in the protein, including 6 Ser,2 Thr and 6 Tyr,. Analysis of its functional domain revealed that there was a superfamily conserved domain of FUT2 protein: FUT1-FUT2-like (amino acid position 58). Phylogenetic tree results showed that the phylogenetic relationship between pigs and cattle was relatively close to that of humans, chimpanzees, rats and mice. FUT2 gene was expressed in 11 tissues of weanling piglets, and the expression level was higher in digestive tract and immune tissues. The results suggest that FUT2 gene may play a certain role in the resistance of weanling piglets to Escherichia coli F18, and may play an indirect role in its resistance to Escherichia coli F18 through the synthesis of fucosyltransferase.
【作者單位】： 揚州大學(xué)江蘇省動物遺傳繁育與分子設(shè)計重點實驗室;江蘇省種豬繁育和健康養(yǎng)殖工程技術(shù)研究中心;
【基金】：國家科技支撐計劃(2015BAD03B01) 江蘇省科技支撐計劃(BE2012330) 蘇州市科技支撐計劃(SNG201410) 太倉市產(chǎn)學(xué)研聯(lián)合創(chuàng)新資金(TC2014CY1)
【分類號】：S828

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