【摘要】：體細(xì)胞胚胎是植物離體再生的高效方式及遺傳轉(zhuǎn)化的理想受體,也是進(jìn)行科學(xué)研究的理想體系;但是從體細(xì)胞轉(zhuǎn)變?yōu)榕咝约?xì)胞的過(guò)程是復(fù)雜的基因調(diào)控網(wǎng)絡(luò)調(diào)控的,在這其中,BABY BOOM/WUSCHEL/LEAFY COTYLEDON/MYB115等轉(zhuǎn)錄因子處于體胚調(diào)控網(wǎng)絡(luò)的上游,能夠帶動(dòng)下游相關(guān)基因的響應(yīng)從而啟動(dòng)了體細(xì)胞胚胎的發(fā)生,起著"畫(huà)龍點(diǎn)睛"的關(guān)鍵作用,是啟動(dòng)體胚發(fā)生"按鈕"和調(diào)控體胚發(fā)育的"開(kāi)關(guān)"。本綜述分析了上述關(guān)鍵基因在啟動(dòng)體細(xì)胞胚胎發(fā)生及體胚發(fā)育和器官再生等過(guò)程中的作用,及探討了從分子水平調(diào)控體胚發(fā)生的研究體系。認(rèn)為,在化學(xué)誘導(dǎo)表達(dá)體系下操縱BABY BOOM和WUSCHEL基因的表達(dá)是啟動(dòng)體胚發(fā)生及提高植物再生能力的最有效的調(diào)控系統(tǒng),無(wú)論同源和異源表達(dá)均表現(xiàn)出明顯的功能和誘導(dǎo)體胚發(fā)生形成的作用,對(duì)于科研和生產(chǎn)均具有重要的價(jià)值和意義。
[Abstract]:Somatic embryo is an efficient way of plant regeneration in vitro and an ideal receptor for genetic transformation. It is also an ideal system for scientific research. But the process of transforming from somatic cells to embryonic cells is regulated by complex gene regulatory networks, in which transcription factors such as BABY BOOM/WUSCHEL/LEAFY COTYLEDON/MYB115 are upstream of somatic regulatory networks. It can stimulate the response of the downstream related genes and start the development of somatic embryos, which plays a key role in promoting the button of somatic embryogenesis and the "switch" of regulating the development of somatic embryos, which plays a key role in promoting somatic embryogenesis and regulating the development of somatic embryos, which plays a key role in the development of somatic embryos. This review reviewed the role of these key genes in the initiation of somatic embryogenesis, somatic embryo development and organ regeneration, and discussed the research system for regulating somatic embryogenesis at the molecular level. It is suggested that controlling the expression of BABY BOOM and WUSCHEL genes under the chemical induced expression system is the most effective regulatory system for promoting somatic embryogenesis and enhancing plant regeneration ability. Both homologue and heterologous expression show obvious function and induce the formation of conductor embryo, which is of great value and significance for scientific research and production.
【作者單位】： 四川農(nóng)業(yè)大學(xué)園林研究所;四川理工學(xué)院生物工程學(xué)院;中國(guó)農(nóng)業(yè)大學(xué)園藝學(xué)院;四川農(nóng)業(yè)大學(xué)生態(tài)林業(yè)研究所;
【基金】：四川省教育廳重點(diǎn)項(xiàng)目(15ZA0023) 四川省“突破性竹類(lèi)花卉育種新材料與方法創(chuàng)新”項(xiàng)目(16ZC2871)共同資助
【分類(lèi)號(hào)】：Q943.2

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