發(fā)布時間：2019-04-04 11:17

【摘要】：生長素響應(yīng)因子(auxin response factor,ARF)基因家族是植物特有的轉(zhuǎn)錄因子家族,在調(diào)控植物生長發(fā)育過程中起重要作用。以公布的甘藍基因組數(shù)據(jù)庫為基礎(chǔ),采用生物信息學(xué)方法鑒定出29個ARF基因,命名為BoARFs,研究分析了其基因結(jié)構(gòu)、染色體分布、蛋白質(zhì)保守結(jié)構(gòu)域、系統(tǒng)進化樹及表達模式,以期為甘藍ARF基因家族功能的深入研究提供參考依據(jù)。結(jié)果表明:BoARF家族基因結(jié)構(gòu)相對復(fù)雜,含有2~14個外顯子;在染色體上呈不均勻分布,除BoARF29基因未定位到染色體上外,其它基因均定位在不同的染色體上;所有BoARF蛋白均具有保守的B3結(jié)構(gòu)域和Auxin_resp結(jié)構(gòu)域,但只有21個BoARF蛋白包含1~2個AUX/IAA結(jié)構(gòu)域;轉(zhuǎn)錄組數(shù)據(jù)表達模式分析表明,ARF基因具有不同的組織表達模式,部分基因表現(xiàn)出組織特異性。
[Abstract]:Auxin response factor (auxin response factor,ARF) gene family is a plant-specific transcription factor family, which plays an important role in the regulation of plant growth and development. Based on the published genomic database of Brassica oleracea, 29 ARF genes were identified by bioinformatics method and named as BoARFs,. The gene structure, chromosome distribution, protein conserved domain, phylogenetic tree and expression pattern were analyzed. In order to provide a reference for the in-depth study of the function of ARF gene family in Brassica oleracea. The results showed that the gene structure of BoARF family was relatively complex, which contained 14 exons, distributed inhomogeneously on chromosomes, except BoARF29 gene did not locate on chromosomes, the other genes were located on different chromosomes, and the other genes were located on different chromosomes except that BoARF29 gene was not located on chromosome. All BoARF proteins had conserved B3 and Auxin_resp domains, but only 21 BoARF proteins contained 1 / 2 AUX/IAA domains. The data expression pattern analysis of the transcript group showed that the ARF gene had different tissue expression patterns, and some of the genes showed tissue specificity.
【作者單位】： 黃河水利職業(yè)技術(shù)學(xué)院;華南農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：廣東省科技計劃資助項目(2015A020209117)
【分類號】：S635
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本文編號：2453755